Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

Western Blot: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponecti

Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.

Flow Cytometry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Adiponectin antibody, and then analyzed by flow cytometry.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.

Immunohistochemistry: Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free [NBP2-71422] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADIPOQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Adiponectin/Acrp30 Antibody (OTI1H1) - Azide and BSA Free

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponectin/Acrp30 Antibody (NBP2-71422)(1)

Publication using NBP2-71422

• Bandyopadhyay S, Duffy MP, Ahn KJ et al. Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging. Cell 2024-06-07 [PMID: 38714197]

Bioinformatics

• Gene Symbol: ADIPOQ