Adiponectin/Acrp30 Antibody (173205) [Unconjugated]

Western blot shows lysates of mouse adipose tissue. PVDF membrane was probed with 2 µg/mL of Mouse Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB1119) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Adiponectin/Acrp30 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 173205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Adiponectin/Acrp30 Antibody (173205) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin
  Glu18-Asn247
  Accession # Q60994
• Specificity: Detects full-length mouse Adiponectin (aa 18‑247) in direct ELISAs and Western blots. Shows no cross-reactivity with recombinant human Adiponectin. Does not detect the C-terminal globular domain of mouse Adiponectin (aa 111‑247).
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ADIPOQ
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponectin/Acrp30 Antibody (173205) [Unconjugated]

• ACDC
• Acrp30
• ACRP30ADPN
• adipocyte, C1Q and collagen domain containing
• Adiponectin
• adiponectin, C1Q and collagen domain containing
• AdipoQ
• ADIPQTL1
• ApM1
• apM-1
• APM1APM-1
• C1q and collagen domain-containing protein
• GBP28
• GBP28apM1
• Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1 - 3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4 - 7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8 - 10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11 - 13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
10. Waki, H. et al. (2005) Endocrinology 146:790.
11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
13. Wang, Y. et al. (2008) Biochem. J. 409:623.
14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adiponectin/Acrp30 Antibody (MAB1119)(5)

Publications using MAB1119

• T Yamamuro, S Nakamura, K Yanagawa, A Tokumura, T Kawabata, A Fukuhara, H Teranishi, M Hamasaki, I Shimomura, T Yoshimori Loss of RUBCN/rubicon in adipocytes mediates the upregulation of autophagy to promote the fasting response Autophagy, 2022-03-14;0(0):1-11. 2022-03-14 [PMID: 35282767] (Immunocytochemistry, Mouse)
• T Yamamuro, T Kawabata, A Fukuhara, S Saita, S Nakamura, H Takeshita, M Fujiwara, Y Enokidani, G Yoshida, K Tabata, M Hamasaki, A Kuma, K Yamamoto, I Shimomura, T Yoshimori Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy Nat Commun, 2020-08-18;11(1):4150. 2020-08-18 [PMID: 32811819] (Western Blot, Mouse)
• Ashley R. DeGroat, Christina K. Fleming, Samantha M. Dunlay, Kendra L. Hagood, Jonathan P. Moorman, Jonathan M. Peterson The sex specific effect of alcohol consumption on circulating levels of CTRP3 PLOS ONE 2018-11-07 [PMID: 30403751] (Western Blot, Mouse)
• R Singh, S Pervin, SJ Lee, A Kuo, V Grijalva, J David, L Vergnes, ST Reddy Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases Diabetes Metab Syndr Obes, 2018-03-26;11(0):65-84. 2018-03-26 [PMID: 29618935] (Western Blot, Mouse)
• Qiao L, Zou C, van der Westhuyzen DR, Shao J Adiponectin reduces plasma triglyceride by increasing VLDL triglyceride catabolism. Diabetes, 2008-03-28;57(7):1824-33. 2008-03-28 [PMID: 18375436] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ADIPOQ
• Uniprot:
  • Q60994(Mouse)