Activin RIB/ALK-4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Activin RIB/ALK-4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Activin RIB/ALK‑4
  Leu32-Glu126
  Accession # AAA60556
• Specificity: Detects human Activin RIB/ALK‑4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVR1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIB/ALK-4 Antibody [Biotin]

• activin A receptor, type IB
• Activin receptor type IB
• Activin receptor-like kinase 4
• Activin RIB
• ActivinRIB
• ACTRIB
• ACVR1B
• ACVRLK4ACTR-IB
• ALK4
• ALK-4
• ALK4activin A receptor, type II-like kinase 4
• EC 2.7.11
• EC 2.7.11.30
• Serine/threonine-protein kinase receptor R2
• SKR2activin receptor type-1B

Background

Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25% - 35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).

1. Chen, Y- G. et al. (2006) Exp. Biol. Med. 231:534. 
2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066. 
3. Tsuchida, K. et al. (1995) Endocrinology 136:5493. 
4. ten Dijke, P. et al. (1993) Oncogene 8:2879. 
5. Xu, J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7957. 
6. Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066. 
7. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
8. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
9. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
10. Lewis, K.A. et al. (2000) Nature 404:411.
11. Gu, Z. et al. (1998) Genes Dev. 12:844.
12. Chen, Y. et al. (2004) Dev. Biol. 268:280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin RIB/ALK-4 Antibody (BAF222)(1)

Publication using BAF222

• LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5 PLoS ONE, 2017-05-10;12(5):e0177188. 2017-05-10 [PMID: 28489883] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ACVR1B
• Uniprot:
  • AAA60556()