Activin RIA/ALK-2/Activin Receptor Type 1 Antibody

Activin RIA/ALK‑2 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using 15 µg/mL Goat Anti-Human Activin RIA/ALK‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF637) overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PC‑3 human prostate cancer cell line was stained with Goat Anti-Human Activin RIA/ALK‑2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF637, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Activin RIA/ALK‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Activin RIA/ALK‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF637) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Activin RIA/ALK-2/Activin Receptor Type 1 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK-2
  Asp23-Glu123
  Accession # Q04771
• Specificity: Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Activin RIB, rhActivin RIIA, and rhActivin RIIB is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVR1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody

• activin A receptor, type I
• Activin RIA
• ActivinRIA
• ACTRI
• ACVR1
• ACVR1A
• ACVRLK2
• ALK2
• ALK-2
• FOP
• SKR1
• TSRI

Background

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (AF637)(4)

Publications using AF637

• P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal Cell Death Dis, 2018;9(9):927. 2018 [PMID: 30206237] (Flow Cytometry, Human)
• L Tengroth, J Arebro, O Larsson, C Bachert, SK Georén, LO Cardell Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps Sci Rep, 2018;8(1):1561. 2018 [PMID: 29367682] (Flow Cytometry, Human)
• Fujimoto M, Ohte S, Shin M, Yoneyama K, Osawa K, Miyamoto A, Tsukamoto S, Mizuta T, Kokabu S, Machiya A, Okuda A, Suda N, Katagiri T Establishment of a novel model of chondrogenesis using murine embryonic stem cells carrying fibrodysplasia ossificans progressiva-associated mutant ALK2. Biochem Biophys Res Commun, 2014;455(3):347-52. 2014 [PMID: 25446088] (Flow Cytometry, Human)
• Masood R, Kumar SR, Sinha UK, Crowe DL, Krasnoperov V, Reddy RK, Zozulya S, Singh J, Xia G, Broek D, Schonthal AH, Gill PS EphB4 provides survival advantage to squamous cell carcinoma of the head and neck. Int. J. Cancer, 2006;119(6):1236-48. 2006 [PMID: 16615113] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ACVR1
• Uniprot:
  • Q04771()