Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Activin RIB/ALK‑4 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of ...read more
Mouse myeloma cell line NS0-derived recombinant human Activin RIB Leu32-Glu126 Accession # AAA60556
Detects human Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Activin RIA is observed and less than 2% cross-reactivity with rhActivin RIIA and rhActivin RIIB is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Activin RIB/ALK-4 Antibody [Unconjugated]
activin A receptor, type IB
Activin receptor type IB
Activin receptor-like kinase 4
ALK4activin A receptor, type II-like kinase 4
Serine/threonine-protein kinase receptor R2
SKR2activin receptor type-1B
Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25% - 35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).
Chen, Y- G. et al. (2006) Exp. Biol. Med. 231:534.
Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
Tsuchida, K. et al. (1995) Endocrinology 136:5493.
ten Dijke, P. et al. (1993) Oncogene 8:2879.
Xu, J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7957.
Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066.
Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
Lewis, K.A. et al. (2000) Nature 404:411.
Gu, Z. et al. (1998) Genes Dev. 12:844.
Chen, Y. et al. (2004) Dev. Biol. 268:280.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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