Activin RIB/ALK-4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Activin RIB/ALK‑4 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Activin RIB/ALK-4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Activin RIB
Leu32-Glu126
Accession # AAA60556
Specificity
Detects human Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Activin RIA is observed and less than 2% cross-reactivity with rhActivin RIIA and rhActivin RIIB is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACVR1B
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Blockade of Receptor-ligand Interaction
  • Knockout Validated
Control
Activin RIB/ALK-4 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF222 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIB/ALK-4 Antibody [Unconjugated]

  • activin A receptor, type IB
  • Activin receptor type IB
  • Activin receptor-like kinase 4
  • Activin RIB
  • ActivinRIB
  • ACTRIB
  • ACVR1B
  • ACVRLK4ACTR-IB
  • ALK4
  • ALK-4
  • ALK4activin A receptor, type II-like kinase 4
  • EC 2.7.11
  • EC 2.7.11.30
  • Serine/threonine-protein kinase receptor R2
  • SKR2activin receptor type-1B

Background

Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25% - 35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).

  1. Chen, Y- G. et al. (2006) Exp. Biol. Med. 231:534. 
  2. Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066. 
  3. Tsuchida, K. et al. (1995) Endocrinology 136:5493. 
  4. ten Dijke, P. et al. (1993) Oncogene 8:2879. 
  5. Xu, J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7957. 
  6. Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066. 
  7. Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
  8. Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
  9. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
  10. Lewis, K.A. et al. (2000) Nature 404:411.
  11. Gu, Z. et al. (1998) Genes Dev. 12:844.
  12. Chen, Y. et al. (2004) Dev. Biol. 268:280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Activin RIB/ALK-4 Antibody (AF222)(3)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF222

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Bioinformatics

Gene Symbol ACVR1B
Uniprot