Actin Gamma 1 Antibody - BSA Free

Western Blot: Actin Gamma 1 Antibody - BSA Free [NBP3-46266] - HeLa cells were subjected to SDS PAGE followed by western blot with (Actin Gamma 1 antibody) at dilution of 1:500

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Actin Gamma 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Actin Gamma 1 Antibody - BSA Free (NBP3-46266) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: actin, gamma 1. (Uniprot# P63261)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:1000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Actin Gamma 1 Antibody - BSA Free

• ACT
• ACTB
• ACTG
• actin, cytoplasmic 2
• actin, gamma 1
• cytoskeletal gamma-actin
• deafness, autosomal dominant 20; deafness, autosomal dominant 26
• DFNA20
• DFNA26
• Gamma-actin

Background

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTG1