ACSL3 Antibody

Images

 
Western Blot: ACSL3 Antibody [NBP2-15252] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - IHC analysis of human breast cancer tissue section, using ACSL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer for 15 ...read more
Simple Western: ACSL3 Antibody [NBP2-15252] - Mouse brain (hippocampus) tissue. Simple Western image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ACSL3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ACSL3. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane; Single-pass type III membrane protein; Peroxisome membrane; Microsome membrane; Endoplasmic reticulum membrane
Predicted Species
Rat (95%), Porcine (95%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Simple Western
  • Western Blot 1:500 - 1:3000
Application Notes
ACSL3 Antibody is validated for Simple Western from a verified customer review.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15252 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ACSL3 Antibody

  • ACS3EC 6.2.1.3
  • acyl-CoA synthetase long-chain family member 3
  • FACL3lignoceroyl-CoA synthase
  • fatty-acid-Coenzyme A ligase, long-chain 3
  • LACS 3
  • LACS3
  • Long-chain acyl-CoA synthetase 3
  • long-chain-fatty-acid--CoA ligase 3
  • PRO2194

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for ACSL3 Antibody (NBP2-15252) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-15252:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western ACSL3 NBP2-15252
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5
reviewed by:
Verified Customer
Simple Western Mouse 07/01/2020
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Summary

ApplicationSimple Western
Sample TestedBrain (hippocampus) tissue
SpeciesMouse
Lot40415

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

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Verified Customer
07/01/2020
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol ACSL3
Uniprot