FACL4 Antibody

Images

 
Western Blot: FACL4 Antibody [NBP2-16401] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using FACL4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Paraffin-embedded mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500.
Western Blot: FACL4 Antibody [NBP2-16401] - Sample (30 ug of whole cell lysate) A: Hela B: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Paraffin-embedded rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500.
Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image from verified customer review.
Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FACL4 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.
Localization
Mitochondrion outer membrane; Single-pass type III membrane protein; Peroxisome membrane; Microsome membrane; Endoplasmic reticulum membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16401 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FACL4 Antibody

  • ACS4mental retardation, X-linked 68
  • acyl-CoA synthetase 4
  • acyl-CoA synthetase long-chain family member 4
  • EC 6.2.1.3
  • FACL4long-chain 4
  • LACS 4
  • LACS4MRX68
  • lignoceroyl-CoA synthase
  • Long-chain acyl-CoA synthetase 4
  • long-chain fatty-acid-Coenzyme A ligase 4
  • long-chain-fatty-acid--CoA ligase 4
  • mental retardation, X-linked 63
  • MRX63

Background

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FACL4 Antibody (NBP2-16401) (0)

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Review for FACL4 Antibody (NBP2-16401) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP2-16401:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence FACL4 NBP2-16401
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reviewed by:
Anonymous
IF Human 04/03/2015
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Summary

ApplicationImmunofluorescence
Sample TestedMcf10A cell line
SpeciesHuman
Lot39568

Blocking

Blocking DetailsPBS 0.5% casein, one hour at room temperature

Primary Anitbody

Dilution Ratio1/500 in blocking buffer, one hour at room temperature

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG (H+L) Alexa594
Secondary Manufacturer Cat#Life Invitrogen A11012
Secondary Concentration1/1000 in blocking buffer

Details

Detection NotesZeiss AxioImagerZ1 apotome, 0.5s exposure
Fixation Detailsmethanol/acetone 10 minutes at -20°C
Wash DescriptionPBS 4 x 5minutes after each antibody

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FACL4 Antibody (NBP2-16401) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FACL4 Antibody (NBP2-16401)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Anonymous
04/03/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACSL4
Uniprot