Western Blot: FACL4 Antibody [NBP2-16401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [C3], C-term diluted at 1:500. Blue: ...read more
Western Blot: FACL4 Antibody [NBP2-16401] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using FACL4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - HBL435 xenograft, using FACL4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15minantibody at 1:500 dilution.
Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody ...read more
FACL4 antibody validated for ICC/IF from a verified customer review.
79 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP2-16401 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 1% BSA, 20% Glycerol (pH7)
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for FACL4 Antibody
ACS4mental retardation, X-linked 68
acyl-CoA synthetase 4
acyl-CoA synthetase long-chain family member 4
Long-chain acyl-CoA synthetase 4
long-chain fatty-acid-Coenzyme A ligase 4
long-chain-fatty-acid--CoA ligase 4
mental retardation, X-linked 63
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for FACL4 Antibody (NBP2-16401)(3)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 2 applications: IHC, WB.
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Publications using NBP2-16401
Tang W, Dong M, Teng F et al. Environmental Allergens House Dust Mites-induced Asthma Causes Ferroptosis in the Lungs Research Square Feb 24 2021 (WB, Mouse)
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