SOST/Sclerostin Antibody [Unconjugated]

Images

 
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL ...read more
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight ...read more
Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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SOST/Sclerostin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
Gln24-Tyr211
Accession # NP_077769
Specificity
Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOST
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1589 in the following applications:

Publications
Read Publications using
AF1589 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOST/Sclerostin Antibody [Unconjugated]

  • sclerostin
  • SOST
  • VBCHsclerosteosis

Background

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOST/Sclerostin Antibody (AF1589)(49)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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ICC
(1)
IHC
(10)
IHC-Fr
(5)
IHC-P
(25)
Western Blot
(8)
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Filter By Species
Mouse
(42)
Rat
(7)
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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using AF1589 Applications Species
J Li, J Xue, Y Jing, M Wang, R Shu, H Xu, C Xue, J Feng, P Wang, D Bai SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone Biomed Res Int, 2019;2019(0):7623562. 2019 [PMID: 31828128] (IHC-P, Mouse) IHC-P Mouse
E Wehrle, DC Tourolle N, GA Kuhn, AC Scheuren, S Hofmann, R Müller Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models Sci Rep, 2019;9(1):17445. 2019 [PMID: 31768003] (IHC-P, Mouse) IHC-P Mouse
DM Pacicca, T Brown, D Watkins, K Kover, Y Yan, M Prideaux, L Bonewald Elevated glucose acts directly on osteocytes to increase sclerostin expression in diabetes Sci Rep, 2019;9(1):17353. 2019 [PMID: 31757981] (IHC, Rat) IHC Rat
CE Metzger, SA Narayanan, JP Elizondo, AM Carter, DC Zawieja, HA Hogan, SA Bloomfield DSS-induced colitis produces inflammation-induced bone loss while irisin treatment mitigates the inflammatory state in both gut and bone Sci Rep, 2019;9(1):15144. 2019 [PMID: 31641205] (IHC, Rat) IHC Rat
KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019;20(1):344. 2019 [PMID: 31351471] (IHC-P, Mouse) IHC-P Mouse
KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis BMC Musculoskelet Disord, 2019;20(1):344. 2019 [PMID: 31351471] (IHC-P, Mouse) IHC-P Mouse
F Ohori, H Kitaura, A Marahleh, A Kishikawa, S Ogawa, J Qi, WR Shen, T Noguchi, Y Nara, I Mizoguchi Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement J Immunol Res, 2019;2019(0):9716758. 2019 [PMID: 31341915] (IHC-P, Mouse) IHC-P Mouse
KM Pineault, JY Song, KM Kozloff, D Lucas, DM Wellik Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life Nat Commun, 2019;10(1):3168. 2019 [PMID: 31320650] (IHC, Mouse) IHC Mouse
L Nguyen-Yam, KI Tanaka, R St-Arnaud, D Goltzman Vitamin D-regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification JCI Insight, 2019;4(13):. 2019 [PMID: 31292298] (IHC-P, Mouse) IHC-P Mouse
S Lotinun, Y Ishihara, K Nagano, R Kiviranta, V Carpentier, L Neff, V Parkman, N Ide, D Hu, P Dann, D Brooks, ML Bouxsein, J Wysolmersk, F Gori, R Baron Cathepsin K-deficient osteocytes prevent lactation-induced bone loss and parathyroid hormone suppression J. Clin. Invest., 2019;130(0):. 2019 [PMID: 31112135] (IHC, Mouse) IHC Mouse
Show All 49 Publications.

Reviews for SOST/Sclerostin Antibody (AF1589) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF1589:
Filter by Applications
WB
(2)
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Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23494985
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23494985

Details

Detection NotesSee PMID 23494985

Comments

CommentsPublished: PMID 23494985
 
reviewed by:
Anonymous
WB Other 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20336693
SpeciesOther

Blocking

Blocking DetailsSee PMID 20336693

Details

Detection NotesSee PMID 20336693

Comments

CommentsPublished: See PMID 20336693

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Secondary Antibodies

 

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Biotin BAF1589

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: WB
Species: Mouse

 
Anonymous
01/05/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol SOST
Uniprot