SOST/Sclerostin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) ...read more
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL ...read more
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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SOST/Sclerostin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
Gln24-Tyr211
Accession # NP_077769
Specificity
Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOST
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1589 in the following applications:

Publications
Read Publications using
AF1589 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOST/Sclerostin Antibody [Unconjugated]

  • sclerostin
  • SOST
  • VBCHsclerosteosis

Background

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOST/Sclerostin Antibody (AF1589)(63)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Xenograft.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IHC
(24)
IHC-Fr
(5)
IHC-P
(25)
Western Blot
(9)
All Applications
Filter By Species
Human
(1)
Mouse
(53)
Rat
(9)
N/A
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using AF1589 Applications Species
Aobulikasimu, A;Liu, T;Piao, J;Sato, S;Ochi, H;Okawa, A;Tsuji, K;Asou, Y; SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruption Scientific reports 2023-05-17 [PMID: 37198221] (IHC, Mouse) IHC Mouse
Lamand�, SR;Ng, ES;Cameron, TL;Kung, LHW;Sampurno, L;Rowley, L;Lilianty, J;Patria, YN;Stenta, T;Hanssen, E;Bell, KM;Saxena, R;Stok, KS;Stanley, EG;Elefanty, AG;Bateman, JF; Modeling human skeletal development using human pluripotent stem cells Proceedings of the National Academy of Sciences of the United States of America 2023-05-09 [PMID: 37126720] (IHC, Xenograft) IHC Xenograft
X Gao, X Sun, H Cheng, JJ Ruzbarsky, M Mullen, M Huard, J Huard MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and Repair International Journal of Molecular Sciences, 2023-01-25;24(3):. 2023-01-25 [PMID: 36768718] (IHC, Mouse) IHC Mouse
X Gao, X Sun, H Cheng, JJ Ruzbarsky, M Mullen, M Huard, J Huard MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and Repair International Journal of Molecular Sciences, 2023;24(3):. 2023 [PMID: 36768718] (IHC, Mouse) IHC Mouse
N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse, N/A) Western Blot Mouse, N/A
EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse) IHC Mouse
T Wei, Z Shan, X Wen, N Zhao, G Shen Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic?induced external apical root resorption in a rat model Molecular Medicine Reports, 2022-05-20;26(1):. 2022-05-20 [PMID: 35593309] (IHC, Rat) IHC Rat
T Moriishi, T Ito, R Fukuyama, X Qin, H Komori, H Kaneko, Y Matsuo, N Yoshida, T Komori Sp7 Transgenic Mice with a Markedly Impaired Lacunocanalicular Network Induced Sost and Reduced Bone Mass by Unloading International Journal of Molecular Sciences, 2022-03-15;23(6):. 2022-03-15 [PMID: 35328592] (IHC, Mouse) IHC Mouse
ZX Wang, ZW Luo, FX Li, J Cao, SS Rao, YW Liu, YY Wang, GQ Zhu, JS Gong, JT Zou, Q Wang, YJ Tan, Y Zhang, Y Hu, YY Li, H Yin, XK Wang, ZH He, L Ren, ZZ Liu, XK Hu, LQ Yuan, R Xu, CY Chen, H Xie Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox Nature Communications, 2022-03-18;13(1):1453. 2022-03-18 [PMID: 35304471] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
JM Kim, YS Yang, J Xie, O Lee, J Kim, J Hong, B Boldyreff, O Filhol, H Chun, MB Greenblatt, G Gao, JH Shim Regulation of sclerostin by the SIRT1 stabilization pathway in osteocytes Cell Death and Differentiation, 2022-02-15;0(0):. 2022-02-15 [PMID: 35169297] (IHC, Mouse) IHC Mouse
Show All 63 Publications.

Reviews for SOST/Sclerostin Antibody (AF1589) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF1589:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23494985
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Other 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20336693
SpeciesOther

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Recent Reviews

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Verified Customer
01/05/2015
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol SOST
Uniprot