SOST/Sclerostin Antibody [Unconjugated]

Images

 
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL ...read more
SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight ...read more
Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOST/Sclerostin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SOST/Sclerostin
Gln24-Tyr211
Accession # NP_077769
Specificity
Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOST
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1589 in the following applications:

Publications
Read Publications using
AF1589 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOST/Sclerostin Antibody [Unconjugated]

  • sclerostin
  • SOST
  • VBCHsclerosteosis

Background

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).

  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOST/Sclerostin Antibody (AF1589)(58)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(18)
IHC-Fr
(5)
IHC-P
(25)
Western Blot
(8)
All Applications
Filter By Species
Human
(1)
Mouse
(50)
Rat
(7)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using AF1589 Applications Species
T Moriishi, T Ito, R Fukuyama, X Qin, H Komori, H Kaneko, Y Matsuo, N Yoshida, T Komori Sp7 Transgenic Mice with a Markedly Impaired Lacunocanalicular Network Induced Sost and Reduced Bone Mass by Unloading International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328592] (IHC, Mouse) IHC Mouse
ZX Wang, ZW Luo, FX Li, J Cao, SS Rao, YW Liu, YY Wang, GQ Zhu, JS Gong, JT Zou, Q Wang, YJ Tan, Y Zhang, Y Hu, YY Li, H Yin, XK Wang, ZH He, L Ren, ZZ Liu, XK Hu, LQ Yuan, R Xu, CY Chen, H Xie Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox Nature Communications, 2022;13(1):1453. 2022 [PMID: 35304471] (Flow Cytometry, Human) Flow Cytometry Human
JM Kim, YS Yang, J Xie, O Lee, J Kim, J Hong, B Boldyreff, O Filhol, H Chun, MB Greenblatt, G Gao, JH Shim Regulation of sclerostin by the SIRT1 stabilization pathway in osteocytes Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35169297] (IHC, Mouse) IHC Mouse
D Zhao, MA Riquelme, T Guda, C Tu, H Xu, S Gu, JX Jiang Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading Elife, 2022;11(0):. 2022 [PMID: 35132953] (IHC, Mouse) IHC Mouse
M Dixit, S Duran-Orti, G Yildirim, SB Poudel, LD Louis, A Bartke, MB Schaffler, JJ Kopchick, S Yakar Induction of somatopause in adult mice compromises bone morphology and exacerbates bone loss during aging Aging Cell, 2021;0(0):e13505. 2021 [PMID: 34811875] (IHC, Mouse) IHC Mouse
E Jackson, N Lara-Casti, MP Akhter, M Dallas, JM Scott, T Ganesh, ML Johnson Osteocyte Wnt/beta-catenin pathway activation upon mechanical loading is altered in ovariectomized mice Bone Reports, 2021;15(0):101129. 2021 [PMID: 34584905] (IHC, Mouse) IHC Mouse
M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi Sclerostin expression in trabecular bone is downregulated by osteoclasts Sci Rep, 2020;10(1):13751. 2020 [PMID: 32792620] (IHC, Mouse) IHC Mouse
M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi Sclerostin expression in trabecular bone is downregulated by osteoclasts Sci Rep, 2020;10(1):13751. 2020 [PMID: 32792620] (IHC, Mouse) IHC Mouse
JY Song, KM Pineault, JM Dones, RT Raines, DM Wellik Hox genes maintain critical roles in the adult skeleton Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020 [PMID: 32170021] (IHC, Mouse) IHC Mouse
J Li, J Xue, Y Jing, M Wang, R Shu, H Xu, C Xue, J Feng, P Wang, D Bai SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone Biomed Res Int, 2019;2019(0):7623562. 2019 [PMID: 31828128] (IHC-P, Mouse) IHC-P Mouse
Show All 58 Publications.

Reviews for SOST/Sclerostin Antibody (AF1589) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF1589:
Filter by Applications
Western Blot
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
Western Blot Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23494985
SpeciesMouse
  4
reviewed by:
Anonymous -
Western Blot Other 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20336693
SpeciesOther

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous -
01/05/2015
Application: Western Blot
Species: Mouse

 
Anonymous -
01/05/2015
Application: Western Blot
Species: Other

Bioinformatics

Gene Symbol SOST
Uniprot