ASNA1 Antibody (2H3)

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3. Lane 1: ASNA1 transfected lysatE (38.8 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Western blot analysis of ASNA1 over-expressed 293 cell line. Lane 2 cotransfected with ASNA1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000439-M03. GAPDH (36.1 kDa) used as loading control.

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in HeLa (Cat # L013V1).

Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in PC-12 (Cat # L012V1).

ELISA: ASNA1 Antibody (2H3) [H00000439-M03] - Detection limit for recombinant GST tagged ASNA1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 2H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ASNA1 Antibody (2H3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ASNA1 (NP_004308, 239 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ
• Specificity: ASNA1 - arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GET3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASNA1 Antibody (2H3)

• arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• ARSA
• ARSA1
• ARSA-I
• Arsenical pump-driving ATPase
• Arsenite-stimulated ATPase
• ATPase ASNA1
• EC 3.6
• EC 3.6.3.16
• GET3
• golgi to ER traffic 3 homolog
• hARSA-I
• hASNA-I
• MGC3821
• Transmembrane domain recognition complex 40 kDa ATPase subunit
• transmembrane domain recognition complex, 40kDa
• TRC40arsA (bacterial) arsenite transporter, ATP-binding, homolog 1

Background

ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASNA1 Antibody (H00000439-M03)(3)

Publications using H00000439-M03

• Ott M, Marques D, Funk C, Bailer SM. Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions. Virol J 2016-10-20 [PMID: 27765046]
• Baron Y, Pedrioli PG, Tyagi K et al. VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase. BMC Biol 2014-05-29 [PMID: 24885147] (ICC/IF, Human)
• Rabu C, Wipf P, Brodsky JL, High S. A precursor specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum. J Biol Chem. 2008-07-29 [PMID: 18667436]

Bioinformatics

• Gene Symbol: GET3
• Entrez:
  • 439(Human)
• OMIM:
  • 601913(Human)
• Uniprot:
  • NP_004308(Human)