Acetyl CoA synthetase Antibody - BSA Free

Immunoprecipitation:Acetyl CoA synthetase AntibodyNBP3-30346] -Detection of human ACSS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACSS2 antibody NBP3-30346 (lot NBP3-30346-1) used for IP at 6 µg per reaction. ACSS2 was also immunoprecipitated by rabbit anti-ACSS2.. For blotting immunoprecipitated ACSS2, NBP3-30346 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Acetyl CoA synthetase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Acetyl CoA synthetase Antibody - BSA Free (NBP3-30346) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 25 and 75
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACSS2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Acetyl CoA synthetase Antibody - BSA Free

• ACAS2
• AceCS
• acetate thiokinase
• acetate-CoA ligase
• Acetate--CoA ligase
• Acetyl-CoA synthetase
• acetyl-Coenzyme A synthetase 2 (ADP forming)
• acetyl-coenzyme A synthetase, cytoplasmic
• ACSA
• ACSACECS
• Acyl-activating enzyme
• acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
• cytoplasmic acetyl-coenzyme A synthetase
• dJ1161H23.1
• DKFZp762G026
• EC 6.2.1

Background

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSS2