ACE/CD143 Antibody (JM59-32)

Western Blot: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of ACE on human lung (1) and mouse kidney (2) tissue lysate using anti-ACE antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded mouse testis tissue using anti-ACE antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded human kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACE/CD143 Antibody (JM59-32) [NBP2-67111] - Analysis of paraffin-embedded mouse kidney tissue using anti-ACE antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: JM59-32
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ACE/CD143 Antibody (JM59-32) Summary

• Description: Novus Biologicals Rabbit ACE/CD143 Antibody (JM59-32) (NBP2-67111) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. Anti-ACE/CD143 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ACE/CD143 aa 1,257-1,306 / 1,306. (SwissProt: P12821 Human; SwissProt: P09470 Mouse)
• Localization: Secreted. Cell membrane. Cytoplasm.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ACE/CD143 Antibody (JM59-32)

• ACE
• ACE1angiotensin converting enzyme, somatic isoform
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• carboxycathepsin
• CD143 antigen
• CD143
• DCP
• DCP1
• DCP1angiotensin-converting enzyme
• dipeptidyl carboxypeptidase 1
• Dipeptidyl carboxypeptidase I
• EC 3.2.1.-
• EC 3.4.15.1
• Kininase II
• MGC26566
• MVCD3
• peptidase P
• testicular ECA

Background

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE/CD143 Antibody (NBP2-67111)(1)

Publication using NBP2-67111

• Yin Y, Cao S, Fu H et al. A noncanonical role of NOD-like receptor NLRP14 in PGCLC differentiation and spermatogenesis Proc Natl Acad Sci U S A 2020-09-08 [PMID: 32839316] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: ACE