ACE-2 Antibody [Allophycocyanin]

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat anti-Human Allophycocyanin-conjugated ACE-2 Polyclonal Antibody (Catalog # FAB933A). Quadrant markers were set based on Goat IgG Isotype Control (IC108A, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

ACE-2 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Allophycocyanin]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (FAB933A)(8)

Publications using FAB933A

• Fernandez, SA;Pelaez-Prestel, HF;Ras-Carmona, A;Mozas-Gutierrez, J;Reyes-Manzanas, R;Reche, PA; Eucalyptus Essential Oil Inhibits Cell Infection by SARS-CoV-2 Spike Pseudotyped Lentivirus Biomedicines 2024-08-19 [PMID: 39200349] (Flow Cytometry, Human)
• Spinello, I;Saulle, E;Quaranta, MT;Pelosi, E;Castelli, G;Cerio, A;Pasquini, L;Morsilli, O;Dupuis, ML;Labbaye, C; AC-73 and Syrosingopine Inhibit SARS-CoV-2 Entry into Megakaryocytes by Targeting CD147 and MCT4 Viruses 2024-01-04 [PMID: 38257782] (Flow Cytometry, Human)
• Laith Ebraham, Chuan Xu, Annie Wang, Cyril Hernandez, Nicholas Siclari, Divino Rajah et al. Oral Epithelial Cells Expressing Low or Undetectable Levels of Human Angiotensin-Converting Enzyme 2 Are Susceptible to SARS-CoV-2 Virus Infection In Vitro Pathogens 6/19/2023 [PMID: 37375533]
• Laith Ebraham, Chuan Xu, Annie Wang, Cyril Hernandez, Nicholas Siclari, Divino Rajah et al. Oral Epithelial Cells Expressing Low or Undetectable Levels of Human Angiotensin-Converting Enzyme 2 Are Susceptible to SARS-CoV-2 Virus Infection In Vitro Pathogens 2023-06-19 [PMID: 37375533] (Flow Cytometry, Human)
• Elena-Sofia Heinl, Sebastian Lorenz, Barbara Schmidt, Nouf Nasser M Laqtom, Joseph R. Mazzulli, Laetitia Francelle et al. CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry iScience 2022-10-21 [PMID: 36093380] (Flow Cytometry, Human)
• Kyu-Young Sim, Gwang-Hoon Ko, So-Eun Bae, Kyu Yeong Choi, Jung Sup Lee, Byeong C. Kim et al. Two Opposing Roles of SARS-CoV-2 RBD-Reactive Antibodies in Pre-Pandemic Plasma Samples From Elderly People in ACE2-Mediated Pseudovirus Infection Frontiers in Immunology 1/11/2022 [PMID: 35087532]
• Anthony R. Cillo, Ashwin Somasundaram, Feng Shan, Carly Cardello, Creg J. Workman, Georgios D. Kitsios et al. People critically ill with COVID-19 exhibit peripheral immune profiles predictive of mortality and reflective of SARS-CoV-2 lung viral burden Cell Reports Medicine 12/2/2021 [PMID: 34873589]
• F Benedetti, G Silvestri, C Mavian, M Weichseldo, A Munawwar, MN Cash, M Dulcey, AY Vittor, M Ciccozzi, M Salemi, OS Latinovic, D Zella Comparison of SARS-CoV-2 Receptors Expression in Primary Endothelial Cells and Retinoic Acid-Differentiated Human Neuronal Cells Viruses, 2021-10-30;13(11):. 2021-10-30 [PMID: 34834998] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9BYF1()