ACE-2 Antibody (AC18F) - Azide and BSA Free

Western Blot: ACE-2 Antibody (AC18F) [NBP2-80035] - Western blot analysis using anti-ACE2 (human), mAb (AC18F) at 1: 2,000 dilution. Lane 1. hACE2 (Fc protein). Lane 2. hACE2 (Ecto domain) (FLAG(R)-tagged). Lane 3. Other hGITR (Fc protein) (control).

Immunohistochemistry-Paraffin: ACE-2 Antibody (AC18F) [NBP2-80035] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80035) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.

Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.

Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: AC18F
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

ACE-2 Antibody (AC18F) - Azide and BSA Free Summary

• Immunogen: Recombinant human ACE-2.
• Specificity: Recognizes human ACE-2. Does not detect recombinant human ACE-2 that has a tag at the C-terminus of the protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACE2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:2000 - 1:10000
  • Flow Cytometry 1:1000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Western blot: Use ECL substrate. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um-filtered solution in PBS, pH 7.4, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ACE-2 Antibody (AC18F) - Azide and BSA Free

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

Angiotensin I Converting Enzyme 2 (ACE2), also known as ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family (theoretical molecular weight 92 kDa). The predicted mouse ACE2 protein sequence consists of 798 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. Mouse ACE2 is 40% homologous in amino acid identity to the N- and C-terminal domains of mouse somatic ACE. Enzymatically, ACE2 converts the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts Ang I to Ang II.

Genetic data from Drosophila, mice and rats show that the ACE2 protein is an essential regulator of heart function in vivo. ACE2 mRNA is found at high levels in testis, kidney and heart with moderate levels in colon, small intestine, and ovary. The ACE2 protein contributes to organ function through the renin-angiotensin system, playing a central role in vascular, renal, and myocardial physiology.

Virology research has demonstrated that the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein binds to its functional receptor, ACE2 (1). Vimentin is a type III intermediate filament protein expressed in mesenchymal cells that helps comprise the cytoskeleton in all animal cells. Studies have demonstrated that vimentin allows cell binding that allows the uptake of SARS-CoV spike protein into a host (2). This direct interaction of SARS-CoV with vimentin has been identified as an entry mechanism for the virus into a host, mediated by the SARS-CoV receptor ACE2 (1,2). It has been shown that ACE2 is the SARS-CoV-2 receptor required for cell entry and plays a physiological role in the replication of SARS-CoV in an infected host (1). Studies using human ACE2 antibody demonstrated a blockade of SARS-CoV-2 and ACE2 interaction, indicating an important physiological component of viral transmission and potential anti-viral therapeutic strategies (3).

References

1. Li, W., Moore, M. J., Vasilieva, N., Sui, J., Wong, S. K., Berne, M. A., . . . Farzan, M. (2003). Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426(6965), 450-454. doi:10.1038/nature02145

2. Yu YT, Chien SC, Chen IY, Lai CT, Tsay YG, Chang SC, Chang MF. (2016) Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci. doi: 10.1186/s12929-016-0234-7

3. Hoffmann, M., Kleine-Weber, H., Schroeder, S., Kruger, N., Herrler, T., Erichsen, S., . . . Pohlmann, S. (2020). SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. doi:10.1016/j.cell.2020.02.052

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (NBP2-80035)(9)

Publications using NBP2-80035

• Saccon TD, Mousovich-Neto F, Ludwig RG et al. SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner Nature communications 2022-09-29 [PMID: 36175400] (WB)
• Bengsch B, Schwabenland M, SaliE H et al. Deep spatial profiling of COVID19 brains reveals neuroinflammation by compartmentalized local immune cell interactions and targets for intervention Research Square Aug 28 2020 (IHC, Human)
• Song X, Hu W, Yu H et al. High expression of angiotensin-converting enzyme-2 (ACE2) on tissue macrophages that may be targeted by virus SARS-CoV-2 in COVID-19 patients Phytomed Plus 2022-04-11 [PMID: 35399819]
• Welch JL, Xiang J, Chang Q et al. Human T cells express Angiotensin Converting Enzyme 2 at levels sufficient to interact with the SARS-CoV-2 Spike protein The Journal of infectious diseases 2021-12-16 [PMID: 34918095] (FLOW)
• Saiz ML, De Diego ML, LOpez-GarcIa D Et al. Epigenetic targeting of the ACE2 and NRP1 viral receptors limits SARS-CoV-2 infectivity Clinical epigenetics 2021-10-11 [PMID: 34635175] (WB)
• Schwabenland M, SaliE H, Tanevski J et al. Deep spatial profiling of COVID19 brains reveals neuroinflammation by compartmentalized local immune cell interactions and targets for intervention Immunity 2021-06-09 [PMID: 34174183]
• Tada T, Fan C, Kaur R et al. A Soluble ACE2 Microbody Protein Fused to a Single Immunoglobulin Fc Domain is a Potent Inhibitor of SARS-CoV-2 Infection Cell Rep 2020-12-16 [PMID: 33326798]
• Song X, Hu W, Yu H et al. Little to no expression of angiotensin-converting enzyme-2 on most human peripheral blood immune cells but highly expressed on tissue macrophages Cytometry. Part A : the journal of the International Society for Analytical Cytology 2020-12-06 [PMID: 33280254] (FLOW, Human)
• Barkama R, Mayo A, Paz A et al. Placenta-Derived Cell Therapy to Treat Patients With Respiratory Failure Due to Coronavirus Disease 2019 Critical care explorations 2020-09-01 [PMID: 32984833] (Human)

Review for ACE-2 Antibody (NBP2-80035) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 07/01/2020

Summary

• Application: Flow Cytometry
• Sample Tested: HEK293 cells
• Species: Human
• Lot: A25981407

Bioinformatics

• Gene Symbol: ACE2