ACE-2 Antibody (2818I) [Unconjugated]

Western blot shows lysates of mouse kidney, mouse ovary, mouse heart, and mouse testis. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Mouse ACE-2 Monoclonal Antibody (Catalog # MAB34372) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

ACE‑2 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with ACE-2 (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Expression of hACE2 in hACE2-KI mouse model. (A) The expression pattern of hACE2 mRNA in different organs including brain, heart, intestines, kidneys, liver, lungs, spleen, stomach, and trachea was detected by real-time quantitative PCR (qPCR) assay. (B) Representative immunoblotting image shows the expression of the ACE2 protein in mouse intestine and lung tissues normalized to total protein. Human ACE2 was highly expressed in the lungs and intestines of hACE2-KI mice, while mouse ACE2 protein was detected in WT mice but not in hACE2-KI mice. (C) Immunochemistry results of the hACE2 expression in different organs. The intestines, lungs, and trachea sections are shown in high-power magnification; scale bar: 100 μm. Statistical analysis was performed using unpaired Student’s t-test or Wilcoxon rank test. NS: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37350787), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF
• Clone: 2818I
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ACE-2 Antibody (2818I) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line CHO-derived mouse ACE-2
  Gln18-Thr740
  Accession # NP_081562
• Specificity: Detects mouse ACE-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (2818I) [Unconjugated]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower, M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
4. Kuba, K. et al. (2005) Nature Med. 11:875.
5. Ima, Y. et al. (2005) Nature 436:112.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (MAB34372)(1)

Publication using MAB34372

• Zhou, X;Sun, W;Zhang, Y;Gu, H;Wang, R;Xie, P;Zhu, Y;Qiu, M;Ding, X;Wang, H;Gao, Y;Li, J; A novel hACE2 knock-in mouse model recapitulates pulmonary and intestinal SARS-CoV-2 infection Frontiers in microbiology 2023-06-06 [PMID: 37350787] (Simple Western, Mouse)

Bioinformatics

• Uniprot:
  • NP_081562()