ABCG8 Antibody

Images

 
Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ...read more
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ABCG8 Antibody Summary

Immunogen
A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
Localization
Integral membrane protein; multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • Immunocytochemistry/Immunofluorescence 1:250
Application Notes
This ABCG8 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot where a doublet is seen at ~67kDa and ~72kDa. Longer exposure times are needed to visualize the wild type ABCG8. For mouse, 100 ug protein (B6/129AKRFVB liver homogenate) loaded with antibody diluted at 1:500. In ICC/IF puncate membrane localization with some weak nuclear reactivity was observed in HEK293 cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ABCG8 Peptide (NB400-117PEP)
Publications
Read Publications using
NB400-117 in the following applications:

  • WB
    6 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCG8 Antibody

  • ATP-binding cassette sub-family G member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • GBD4ATP-binding cassette, subfamily G, member 8
  • MGC142217
  • sterolin 2
  • sterolin-2
  • STSL

Background

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-117
Species: Hu, Mu
Applications: WB, ICC/IF

Publications for ABCG8 Antibody (NB400-117)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
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Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB400-117 Applications Species
Sachdev V, Leopold C, Bauer R et al. Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Jun 23 2016 [PMID: 27344248] (WB) WB
Heverin M, Ali Z, Olin M et al. On the regulatory importance of 27-hydroxycholesterol in mouse liver J. Steroid Biochem. Mol. Biol. Feb 3 2016 [PMID: 26851362] (WB, Mouse) WB Mouse
Huang Y, Wang J, Quan G et al. Lactobacillus acidophilus ATCC 4356 prevents atherosclerosis via inhibition of intestinal cholesterol absorption in Apolipoprotein E-knockout mice. Appl. Environ. Microbiol. 2014 Sep 26 [PMID: 25261526] (WB, Mouse)

Details:
ABCG8 antibody used for WB on total proteins isolated from small intestines of ApoE?/? mice treated with or without Lactobacillus acidophilus / ATCC 4356 (FIG 7C).
WB Mouse
Bujold K, Mellal K, Zoccal KF et al. EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice. Atherosclerosis 2013 Aug [PMID: 23880196] (WB, Mouse) WB Mouse
Levy E, Ouadda AB, Spahis S et al. PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Atherosclerosis 2013 Feb 4 [PMID: 23422832] (WB, Human) WB Human
Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012 [PMID: 22844422] (WB, Human) WB Human
Mathur, SN, Watt, KR, Field, FJ. Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid. J Lipid Res;48(2):395-404. 2007 Feb. [PMID: 17114806]

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Product General Protocols

View specific protocols for ABCG8 Antibody (NB400-117): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCG8 Antibody (NB400-117) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-117AF405
Alexa Fluor 488 Labeled NB400-117AF488
Alexa Fluor 647 Labeled NB400-117AF647
Alexa Fluor 700 Labeled NB400-117AF700
Biotin Labeled NB400-117B
DyLight 405 Labeled NB400-117V
DyLight 488 Labeled NB400-117G
DyLight 550 Labeled NB400-117R
DyLight 650 Labeled NB400-117C
FITC Labeled NB400-117F
HRP Labeled NB400-117H

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Bioinformatics Tool for ABCG8 Antibody (NB400-117)

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Blogs on ABCG8.

Understanding Sitosterolemia: How ABCG5-ABCG8 Dimer Affects Blood Sterol Levels
The ATP binding cassette (ABC) transporter family is the largest and most diverse family of membrane transport proteins and, as the name suggests, uses the energy generated by ATP hydrolysis to transport substrates across membranes. Eukaryotic ABC tra...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.

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Bioinformatics

Gene Symbol ABCG8