Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ...read more
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.02% Sodium Azide
Immunogen affinity purified
Western Blot 1:250-1:500
This ABCG8 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot where a doublet is seen at ~67kDa and ~72kDa. Longer exposure times are needed to visualize the wild type ABCG8. For mouse, 100 ug protein (B6/129AKRFVB liver homogenate) loaded with antibody diluted at 1:500. In ICC/IF puncate membrane localization with some weak nuclear reactivity was observed in HEK293 cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
ATP-binding cassette, sub-family G (WHITE), member 8
GBD4ATP-binding cassette, subfamily G, member 8
ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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