Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.
Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.
Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Decrease in the biliary cholesterol secretion in L1-Tg mice. Immunoblot detection of hepatic NPC1L1 protein in L1-Tg mice using an anti-NPC1L1 ...read more
In Western blot a band is observed at ~140 kDa representing NPC1L1, and a non-specific band may be seen ~60 kDa.
140 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Niemann-Pick type C1 Like-1 Antibody - BSA Free
Niemann-Pick C1-like protein 1
NPC1 (Niemann-Pick disease, type C1, gene)-like 1
Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Niemann-Pick type C1 Like-1 Antibody (NB400-128). (Showing 1 - 2 of 2 FAQs).
I am currently working with samples from pigs and wants to investigate the cholesterol transporter NPC1L1 by Western blot. For pig, I could not find a specific antibody, so I look for a sequence homology with the pig. Can you give me information on the antigens of the following antibodies: Niemann-Pick type C1 Like-1 Antibody NB400-127 and NB400-128?
I ran a blast on the human protein and the pig protein and there is 80% homology between the two proteins. Our lab only recommends using if the percentage homology is 85% or above. You may wish to test either antibody and you would be eligble for our Innovators Reward Program in which you would gain a 50% refund on the product and a 50% discount on another product. All you would need to do is email your results, (successful or unsuccesful) to firstname.lastname@example.org. The amino acid range for the immunogen for NB400-127 is 1000-1100 amino acids and that for NB400-128 is 500- 600.
Can I please get the epitope of your NB400-128?
The exact sequence of this product is proprietary, but I can provide you with the amino acid range. It was made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [UniProt# Q6T3U3].
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