Niemann-Pick type C1 Like-1 Antibody

Images

 
Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). Lane 1: 2 ug/ml. Lane 2: 4 ug/ml. ECL: 30 minute exposure.
Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of small intestine, enterocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Niemann-Pick type C1 Like-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [UniProt# Q6T3U3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
Application Notes
This Niemann-Pick type C1 Like-1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot. In Western blot a band is observed at ~140 kDa representing NPC1L1, and a non-specific band may be seen ~60 kDa.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Niemann-Pick type C1 Like-1 Peptide (NB400-128PEP)
Publications
Read Publications using
NB400-128 in the following applications:

  • IP
    1 publication
  • WB
    14 publications

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Niemann-Pick type C1 Like-1 Antibody

  • Niemann-Pick C1-like protein 1
  • NPC1 (Niemann-Pick disease, type C1, gene)-like 1
  • NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-527
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NB400-128
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP

Publications for Niemann-Pick type C1 Like-1 Antibody (NB400-128)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(14)
All Applications
Filter By Species
Human
(10)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB400-128 Applications Species
Soayfane Z, Terce F, Cantiello M et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 2016 Jul 28 [PMID: 27478484] (WB) WB
Kawase A, Hata S, Takagi M. Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption. Journal of Pharmacy & Pharmaceutical Sciences. 2015 Nov 08 [PMID: 26670369] (WB, Human) WB Human
Sane A, Seidman E, Spahis S et al. New Insights in Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis J. Cell. Biochem. 2015 Mar 30 [PMID: 25826777] (WB, Human) WB Human
Sui Y, Helsley RN, Park SH et al. Intestinal pregnane X receptor links xenobiotic exposure and hypercholesterolemia. Mol. Endocrinol. 2015 Mar 26 [PMID: 25811240] (WB, Mouse) WB Mouse
Takada T, Yamanashi Y, Konishi K et al. NPC1L1 is a key regulator of intestinal vitamin K absorption and a modulator of warfarin therapy Sci Transl Med. 2015 Feb 18 [PMID: 25696002]
Ahn Sang Bong, Jang Kiseok, Jun Dae Won et al. Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease. Dig Dis Sci. 2014 Aug 08 [PMID: 25102981] (Human) Human
Bujold K, Mellal K, Zoccal KF et al. EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice. Atherosclerosis 2013 Aug [PMID: 23880196] (WB, Mouse) WB Mouse
Levy E, Ouadda AB, Spahis S et al. PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Atherosclerosis 2013 Feb 4 [PMID: 23422832] (WB, Human)

Details:
Using the HRP conjugated version of NB400-128, catalog number NB400-128H.
WB Human
Malhotra P, Boddy CS, Soni V et al. D-Glucose Modulates Intestinal Niemann-Pick C1 Like 1 (NPC1L1) Gene Expression via Transcriptional Regulation Am J Physiol Gastrointest Liver Physiol 2012 Nov 8 [PMID: 23139223] (WB, Human) WB Human
Yoon HS, Ju JH, Kim HN et al. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods Int J Food Sci Nutr 2012 Jul 20 [PMID: 22816655] (WB, Human) WB Human
Show All 17 Publications.

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Product General Protocols

View specific protocols for Niemann-Pick type C1 Like-1 Antibody (NB400-128): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Niemann-Pick type C1 Like-1 Antibody (NB400-128). (Showing 1 - 2 of 2 FAQs).

  1. I am currently working with samples from pigs and wants to investigate the cholesterol transporter NPC1L1 by Western blot. For pig, I could not find a specific antibody, so I look for a sequence homology with the pig. Can you give me information on the antigens of the following antibodies: Niemann-Pick type C1 Like-1 Antibody NB400-127 and NB400-128?
    • I ran a blast on the human protein and the pig protein and there is 80% homology between the two proteins. Our lab only recommends using if the percentage homology is 85% or above. You may wish to test either antibody and you would be eligble for our Innovators Reward Program in which you would gain a 50% refund on the product and a 50% discount on another product. All you would need to do is email your results, (successful or unsuccesful) to innovators@novusbio.com. The amino acid range for the immunogen for NB400-127 is 1000-1100 amino acids and that for NB400-128 is 500- 600.
  2. Can I please get the epitope of your NB400-128?
    • The exact sequence of this product is proprietary, but I can provide you with the amino acid range. It was made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [UniProt# Q6T3U3].

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-128AF405
Alexa Fluor 488 Labeled NB400-128AF488
Alexa Fluor 647 Labeled NB400-128AF647
Alexa Fluor 700 Labeled NB400-128AF700
Biotin Labeled NB400-128B
DyLight 405 Labeled NB400-128V
DyLight 488 Labeled NB400-128G
DyLight 550 Labeled NB400-128R
DyLight 650 Labeled NB400-128C
FITC Labeled NB400-128F
HRP Labeled NB400-128H

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Bioinformatics

Gene Symbol NPC1L1