Niemann-Pick type C1 Like-1 Antibody - BSA Free

Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.

Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.

Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Decrease in the biliary cholesterol secretion in L1-Tg mice. Immunoblot detection of hepatic NPC1L1 protein in L1-Tg mice using an anti-NPC1L1 (Niemann-Pick type C1 Like-1) antibody. WT, wild-type. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/s12944-019-1179-0), licensed under a CC-BY license.

Western Blot: Niemann-Pick type C1 Like-1 Antibody - BSA Free [NB400-128] - Hepatic NPC1L1‐mediated steatosis in the liver of L1‐Tg mice. A, Hepatic expression of the human NPC1L1 protein in L1‐Tg mice demonstrated by immunoblotting using an anti‐NPC1L1 antibody. alpha ‐Tubulin, a loading control. B, Decrease of biliary cholesterol levels in L1‐Tg mice. Data are expressed as the mean ± SEM n = 6 (WT) & 7 (L1‐Tg). C, Photographic images of the livers of WT & L1‐Tg mice fed a control fat diet (CFD) or a high‐fat diet (HFD) for 2 weeks. The coin diameter was 1 cm. D, Hematoxylin & eosin (H&E) & Oil Red O staining of the livers of WT & L1‐Tg mice fed a HFD for 2 weeks. E & F, Hepatic cholesterol levels (E, left), hepatic triglyceride (TG) levels (E, right), body weight (BW) (F, left), & the ratios of liver weight to BW (L/B ratio) (F, right) in each group of mice fed a CFD or HFD for 2 weeks. Data are expressed as the mean ± SEM n = 11 (WT‐CFD) & 7 (the other groups). Statistical analyses for significant differences were performed using Bartlett's test, followed by a parametric Tukey–Kramer multiple‐comparison test (E, right; F) or a non‐parametric Steel–Dwass test (E, left) (##P < 0.01 vs CFD controls; **P < 0.01 vs. the other groups; *P < 0.05 among two groups; NS, not significantly different among groups) as well as a two‐sided t test (††P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32123832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Niemann-Pick type C1 Like-1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [Uniprot: Q6T3U3]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPC1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: In Western blot a band is observed at ~140 kDa representing NPC1L1, and a non-specific band may be seen ~60 kDa.
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Niemann-Pick type C1 Like-1 Peptide (NB400-128PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Niemann-Pick type C1 Like-1 Antibody - BSA Free

• Niemann-Pick C1-like protein 1
• NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Niemann-Pick type C1 Like-1 Antibody (NB400-128)(26)

Publications using NB400-128

• Zhang J, Tian R, Liu J, Yuan J et Al. A two-front nutrient supply environment fuels small intestinal physiology through differential regulation of nutrient absorption and host defense Cell 2024-10-20 [PMID: 39427662]
• Zhang Z, Wen H, Peng B, et al. High-fat diet-induced TRAF6 Upregulation Promotes Liver Cholesterol Accumulation and Fatty Liver Development Through EZH2-mediated miR-429/PPAR alpha axis Mol Ther Nucleic Acids 2021-05-17 [PMID: 33996254]
• Toyoda Y, Takada T et al. Identification of hepatic NPC1L1 as an NAFLD risk factor evidenced by ezetimibe-mediated steatosis prevention and recovery. FASEB Bioadv 2019-01-05 [PMID: 32123832] (WB, Mouse)
• Toyoda Y, Takada T, Yamanashi Y, Suzuki H Pathophysiological importance of bile cholesterol reabsorption: hepatic NPC1L1-exacerbated steatosis and decreasing VLDL-TG secretion in mice fed a high-fat diet Lipids Health Dis 2019-12-28 [PMID: 31883528] (WB, Mouse)
• Koo M, Olevsky O, Ruchalski K, Song S Hepatic expression of Niemann-Pick C1-Like 1, a cholesterol re-absorber from bile, exacerbates western diet-induced atherosclerosis in LDL receptor mutant mice Mol. Pharmacol. 2019-05-07 [PMID: 31064810] (WB, Mouse)
• Meng Z, Gwag T, Sui Y et al. The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling JCI Insight 2019-02-07 [PMID: 30728326] (WB, Human)
• Ontawong A, Duangjai A, Muanprasat C et al. Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats. Phytomedicine 2018-06-01 [PMID: 30599898] (WB, Human, Rat)
• Ahn SB, Jun DW, Jang K et al. Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease Korean J. Intern. Med. 2019-07-01 [PMID: 29466845] (IHC-P, Human)
• Soayfane Z, Terce F, Cantiello M et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 2016-07-28 [PMID: 27478484] (WB)
• Kawase A, Hata S, Takagi M. Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption. Journal of Pharmacy & Pharmaceutical Sciences. 2015-11-08 [PMID: 26670369] (WB, Human)
• Sane A, Seidman E, Spahis S et al. New Insights in Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis J. Cell. Biochem. 2015-03-30 [PMID: 25826777] (WB, Human)
• Sui Y, Helsley RN, Park SH et al. Intestinal pregnane X receptor links xenobiotic exposure and hypercholesterolemia. Mol. Endocrinol. 2015-03-26 [PMID: 25811240] (WB, Mouse)
• Takada T, Yamanashi Y, Konishi K et al. NPC1L1 is a key regulator of intestinal vitamin K absorption and a modulator of warfarin therapy Sci Transl Med. 2015-02-18 [PMID: 25696002]
• Ahn Sang Bong, Jang Kiseok, Jun Dae Won et al. Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease. Dig Dis Sci. 2014-08-08 [PMID: 25102981] (Human)
• Bujold K, Mellal K, Zoccal KF et al. EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice. Atherosclerosis 2013-08-01 [PMID: 23880196] (WB, Mouse)
• Levy E, Ouadda AB, Spahis S et al. PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Atherosclerosis 2013-02-04 [PMID: 23422832] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB400-128, catalog number NB400-128H.
• Hager L, Li L, Pun H et al. Lecithin:Cholesterol Acyltransferase Deficiency Protects against Cholesterol-induced Hepatic Endoplasmic Reticulum Stress in Mice J Biol Chem 2012-06-08 [PMID: 22500017] (WB, Mouse)
• Liu W, Mani S, Davis NR et al. Mutation in EGFP Domain of LDL Receptor-Related Protein 6 Impairs Cellular LDL Clearance. Circ Res;103(11):1280-1288. 2008-01-01 [PMID: 18948618] (IP, Human)
• Infante, R E et al. Purified NPC1 protein. I. Binding of cholesterol and oxysterols to a 1278-amino acid membrane protein. J Biol Chem. 283(2):1052-63. Epub 2007 Nov 6. 2008-01-11 [PMID: 17989073] (WB, Human)
• Helsley RN. THE ROLE OF PXR AND IKKbeta SIGNALING IN CARDIOMETABOLIC DISEASE. Thesis. 2016-01-01 (WB, Mouse)
• Pan X, Jiang X C et al. Impaired cholesterol metabolism and enhanced atherosclerosis in clock mutant mice. Circulation 2013-10-15 [PMID: 24014832] (WB, Mouse)
• Malhotra P, Boddy CS, Soni V et al. D-Glucose Modulates Intestinal Niemann-Pick C1 Like 1 (NPC1L1) Gene Expression via Transcriptional Regulation Am J Physiol Gastrointest Liver Physiol 2012-11-08 [PMID: 23139223] (WB, Human)
• Yoon HS, Ju JH, Kim HN et al. Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods Int J Food Sci Nutr 2012-07-20 [PMID: 22816655] (WB, Human)
• Duangjai A, Ingkaninan K, Praputbut S, Limpeanchob N. Black pepper and piperine reduce cholesterol uptake and enhance translocation of cholesterol transporter proteins J Nat Med 2012-06-27 [PMID: 22736065] (WB, Human)
• Montoudis A, Seidman E, Boudreau F et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008-05-01 [PMID: 18235139] (WB, Human)
• Nassir F, Wilson B, Han X et al. CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine. J Biol Chem;282(27):19493-501. 2007-07-06 [PMID: 17507371] (WB, Mouse)

FAQs for Niemann-Pick type C1 Like-1 Antibody (NB400-128). (Showing 1 - 2 of 2 FAQs).

1. I am currently working with samples from pigs and wants to investigate the cholesterol transporter NPC1L1 by Western blot. For pig, I could not find a specific antibody, so I look for a sequence homology with the pig. Can you give me information on the antigens of the following antibodies: Niemann-Pick type C1 Like-1 Antibody NB400-127 and NB400-128?
   • I ran a blast on the human protein and the pig protein and there is 80% homology between the two proteins. Our lab only recommends using if the percentage homology is 85% or above. You may wish to test either antibody and you would be eligble for our Innovators Reward Program in which you would gain a 50% refund on the product and a 50% discount on another product. All you would need to do is email your results, (successful or unsuccesful) to innovators@novusbio.com. The amino acid range for the immunogen for NB400-127 is 1000-1100 amino acids and that for NB400-128 is 500- 600.
2. Can I please get the epitope of your NB400-128?
   • The exact sequence of this product is proprietary, but I can provide you with the amino acid range. It was made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 500-600). [UniProt# Q6T3U3].

Bioinformatics

• Gene Symbol: NPC1L1