ABCG5 Antibody (1B5E10)

Images

 
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate.
Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
Flow Cytometry: ABCG5 Antibody (1B5E10) [NBP2-23488] - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HEK293 and (2) ABCG5 hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded human cervical cancer cancer tissues with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, ICC/IF (-)
Clone
1B5E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ABCG5 Antibody (1B5E10) Summary

Immunogen
Partial recombinant human ABCG5 (between residues 300-500) expressed in E. coli [UniProt Q9H222].
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCG5
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
Control
Jurkat Whole Cell Lysate
Raji Whole Cell Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% proprietary protein stabilizer
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for ABCG5 Antibody (1B5E10)

  • ATP-binding cassette sub-family G member 5
  • ATP-binding cassette, sub-family G (WHITE), member 5
  • ATP-binding cassette, subfamily G, member 5
  • sterolin 1
  • sterolin-1
  • STSL

Background

ABCG5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC proteins are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCG5 is a member of the White subfamily. It functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. ABCG5 is expressed in a tissue-specific manner in the liver, colon, and intestine. Mutations in this protein may contribute to sterol accumulation and atheroschlerosis, and they have been observed in patients with sitosterolemia (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCG5 Antibody (NBP2-23488) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ABCG5 Antibody (NBP2-23488) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-23488AF350
Alexa Fluor 405 NBP2-23488AF405
Alexa Fluor 488 NBP2-23488AF488
Alexa Fluor 532 NBP2-23488AF532
Alexa Fluor 594 NBP2-23488AF594
Alexa Fluor 647 NBP2-23488AF647
Alexa Fluor 700 NBP2-23488AF700
Alexa Fluor 750 NBP2-23488AF750
Allophycocyanin NBP2-23488APC
Biotin NBP2-23488B
DyLight 350 NBP2-23488UV
DyLight 405 NBP2-23488V
DyLight 488 NBP2-23488G
DyLight 550 NBP2-23488R
DyLight 594 NBP2-23488DL594
DyLight 650 NBP2-23488C
DyLight 680 NBP2-23488FR
DyLight 755 NBP2-23488IR
FITC NBP2-23488F
HRP NBP2-23488H
Janelia Fluor 549 NBP2-23488JF549
Janelia Fluor 646 NBP2-23488JF646
PE NBP2-23488PE
PerCP NBP2-23488PCP

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Bioinformatics

Gene Symbol ABCG5
Entrez
Uniprot