ABCE1 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody [NB400-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

ABCE1 Antibody - BSA Free Summary

Immunogen
A fusion protein containing amino acids 1-119 of human ABCE1 [UniProt# P61221]
Localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunoprecipitation 1:1000
  • Western Blot 1:250-1:1000
Application Notes
This ABCE1 antibody is useful for Immunoprecipitaton, Immunocytochemistry/Immunofluorescence, and Western Blot. In ICC/IF, mitochondrial staining was observed in HeLa cells. In Western Blot, a band was seen ~68 kD representing ABCE1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
HeLa Whole Cell Lysate
ABCE1 Overexpression Lysate
Publications
Read Publications using
NB400-116 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 25227463).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCE1 Antibody - BSA Free

  • 2'-5'-oligoadenylate-binding protein
  • ATP-binding cassette, sub-family E (OABP), member 1
  • huHP68
  • OABP
  • Ribonuclease 4 inhibitor
  • ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor
  • RLIABC38
  • RNase L inhibitor
  • RNASEL1
  • RNASELIRNS4IATP-binding cassette sub-family E member 1

Background

ABCE1 (ATP-binding cassette sub-family E member 1), also named RLI or HP68, belongs to ABC transporter superfamily/ABCE family and is composed of two nucleotide binding domains and an N-terminal Fe-S binding domain. ABCE1 heterodimerizes with RNASEL leading to its inhibition and has the ability to associates with HIV-1 Vif, HIV-1, HIV-2 and SIV Gag proteins. Evolutionarily highly conserved, ABCE1 localize to cytoplasm/mitochondrion as well as to virus formation clusters at plasma membrane and its highest expression has been observed in brain, kidney, prostate etc. where it gets activated by HIV-1 or EMCV (encephalomyocarditis virus). Originally identified as an RNase L inhibitor, ABCE1 has now been implicated in more fundamental processes of translation and ribosome biogenesis, and can regulate functions including viral infection/assembly of immature HIV-1 capsids, tumor cell proliferation, anti-apoptosis and is also a candidate for tumor-suppressor protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-351
Species: Ha, Hu, Mu(-), Pm, Rt(-)
Applications: ELISA, IHC, IHC-P, KO, WB
NBP2-58327
Species: Hu, Mu
Applications: ICC/IF, WB
NB400-156
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IP, WB
NBP1-31113
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
247-ILB/CF
Species: Hu
Applications: BA
NBP2-86906
Species: Hu
Applications: IHC, IHC-P, WB
AF5059
Species: Hu
Applications: IHC, WB
NBP1-77687
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
375-TL
Species: Hu
Applications: BA
NBP2-37923
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-1471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
NBP2-67698
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF6989
Species: Mu
Applications: IHC
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
H00342184-M07
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP2-67667
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
285-IF
Species: Hu
Applications: BA
NB400-115
Species: Hu, Mu, Ye
Applications: ICC/IF, KD, Simple Western, WB
NBP1-87258
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB

Publications for ABCE1 Antibody (NB400-116)(5)

Reviews for ABCE1 Antibody (NB400-116) (0)

There are no reviews for ABCE1 Antibody (NB400-116). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCE1 Antibody (NB400-116). (Showing 1 - 1 of 1 FAQs).

  1. Could you please provide me with Immunprecipitation protocol specific for ABCE1 Antibody (NB400-116)?
    • We do not have a product specific Immunoprecipitation protocol for this product, however we do have a general IP protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional ABCE1 Products

Bioinformatics Tool for ABCE1 Antibody (NB400-116)

Discover related pathways, diseases and genes to ABCE1 Antibody (NB400-116). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ABCE1 Antibody (NB400-116)

Discover more about diseases related to ABCE1 Antibody (NB400-116).
 

Pathways for ABCE1 Antibody (NB400-116)

View related products by pathway.

PTMs for ABCE1 Antibody (NB400-116)

Learn more about PTMs related to ABCE1 Antibody (NB400-116).
 

Research Areas for ABCE1 Antibody (NB400-116)

Find related products by research area.

Blogs on ABCE1

There are no specific blogs for ABCE1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ABCE1 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol ABCE1