ABCE1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody [NB400-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

ABCE1 Antibody Summary

Immunogen
A fusion protein containing amino acids 1-119 of human ABCE1 [UniProt# P61221]
Localization
Cytoplasm. Mitochondrion. Localized to clusters of virus formation at the plasma membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunoprecipitation 1:1000
Application Notes
This ABCE1 antibody is useful for Immunoprecipitaton, Immunocytochemistry/Immunofluorescence, and Western Blot. In ICC/IF, mitochondrial staining was observed in HeLa cells. In Western Blot, a band was seen ~68 kD representing ABCE1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
HeLa Whole Cell Lysate
ABCE1 Overexpression Lysate
Publications
Read Publications using
NB400-116 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 25227463).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCE1 Antibody

  • 2'-5'-oligoadenylate-binding protein
  • ATP-binding cassette, sub-family E (OABP), member 1
  • huHP68
  • OABP
  • Ribonuclease 4 inhibitor
  • ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor
  • RLIABC38
  • RNase L inhibitor
  • RNASEL1
  • RNASELIRNS4IATP-binding cassette sub-family E member 1

Background

ABCE1 (ATP-binding cassette sub-family E member 1), also named RLI or HP68, belongs to ABC transporter superfamily/ABCE family and is composed of two nucleotide binding domains and an N-terminal Fe-S binding domain. ABCE1 heterodimerizes with RNASEL leading to its inhibition and has the ability to associates with HIV-1 Vif, HIV-1, HIV-2 and SIV Gag proteins. Evolutionarily highly conserved, ABCE1 localize to cytoplasm/mitochondrion as well as to virus formation clusters at plasma membrane and its highest expression has been observed in brain, kidney, prostate etc. where it gets activated by HIV-1 or EMCV (encephalomyocarditis virus). Originally identified as an RNase L inhibitor, ABCE1 has now been implicated in more fundamental processes of translation and ribosome biogenesis, and can regulate functions including viral infection/assembly of immature HIV-1 capsids, tumor cell proliferation, anti-apoptosis and is also a candidate for tumor-suppressor protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCE1 Antibody (NB400-116)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCE1 Antibody (NB400-116). (Showing 1 - 1 of 1 FAQs).

  1. Could you please provide me with Immunprecipitation protocol specific for ABCE1 Antibody (NB400-116)?
    • We do not have a product specific Immunoprecipitation protocol for this product, however we do have a general IP protocol.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-116AF350
Alexa Fluor 405 NB400-116AF405
Alexa Fluor 488 NB400-116AF488
Alexa Fluor 532 NB400-116AF532
Alexa Fluor 594 NB400-116AF594
Alexa Fluor 647 NB400-116AF647
Alexa Fluor 700 NB400-116AF700
Alexa Fluor 750 NB400-116AF750
Biotin NB400-116B
DyLight 350 NB400-116UV
DyLight 405 NB400-116V
DyLight 488 NB400-116G
DyLight 550 NB400-116R
DyLight 594 NB400-116DL594
DyLight 650 NB400-116C
DyLight 680 NB400-116FR
DyLight 755 NB400-116IR
FITC NB400-116F
HRP NB400-116H

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Contact Information

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Bioinformatics

Gene Symbol ABCE1