Western Blot: ABCE1 Antibody [NB400-116] - WB analysis of ABCE1 in A431 lysate.
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody [NB400-116] - ABCE1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:250-1:1000
This ABCE1 antibody is useful for Immunoprecipitaton, Immunocytochemistry/Immunofluorescence, and Western Blot. In ICC/IF, mitochondrial staining was observed in HeLa cells. In Western Blot, a band was seen ~68 kD representing ABCE1.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
ATP-binding cassette, sub-family E (OABP), member 1
Ribonuclease 4 inhibitor
ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor
RNase L inhibitor
RNASELIRNS4IATP-binding cassette sub-family E member 1
ABCE1 (ATP-binding cassette sub-family E member 1), also named RLI or HP68, belongs to ABC transporter superfamily/ABCE family and is composed of two nucleotide binding domains and an N-terminal Fe-S binding domain. ABCE1 heterodimerizes with RNASEL leading to its inhibition and has the ability to associates with HIV-1 Vif, HIV-1, HIV-2 and SIV Gag proteins. Evolutionarily highly conserved, ABCE1 localize to cytoplasm/mitochondrion as well as to virus formation clusters at plasma membrane and its highest expression has been observed in brain, kidney, prostate etc. where it gets activated by HIV-1 or EMCV (encephalomyocarditis virus). Originally identified as an RNase L inhibitor, ABCE1 has now been implicated in more fundamental processes of translation and ribosome biogenesis, and can regulate functions including viral infection/assembly of immature HIV-1 capsids, tumor cell proliferation, anti-apoptosis and is also a candidate for tumor-suppressor protein.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.