ABCB5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody [NBP1-77687] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunohistochemistry: ABCB5 Antibody [NBP1-77687] - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ABCB5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ABCB5 Antibody - BSA Free (NBP1-77687) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ABCB5 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of the human ABCB5 protein (between residues 150-200) [UniProt Q2M3G0]
• Localization: Cell membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot reported in scientific literature (PMID 25030174)
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCB5 Antibody - BSA Free

• ABCB5 P-gp
• ABCB5
• ABCB5alpha
• ABCB5beta
• ATP-binding cassette protein
• ATP-binding cassette sub-family B member 5
• ATP-binding cassette, sub-family B (MDR/TAP), member 5
• EC 3.6.3
• EC 3.6.3.44
• EST422562
• P-glycoprotein ABCB5

Background

The ATP-binding cassette sub-family B member 5 (ABCB5) is an ABC transporter and PGP member. It is highly expressed in skin and in malignant melanoma. When two abnormal growths of tissues occur, resulting in multiple malignancies, ABCB5 is often highly expressed. This elevated expression in tumor cells can indicate a propensity towards therapeutic failure in many cases. Analysis of ABCB5 concentrations in tumors has been suggested as a fairly dependable qualitative biomarker for assessing the malignancy of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB5 Antibody (NBP1-77687)(7)

Publications using NBP1-77687

• Silvani G, Kopecky C, Romanazzo S et al. Capillary constrictions prime cancer cell tumorigenicity through PIEZO1 Nature Communications 2025-09-01 [PMID: 40890143]
• Lasagni Vitar R, Triani F, Barbariga M et al. Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence Stem cell reports 2022-03-10 [PMID: 35334220]
• Strobel SB, Machiraju D, HUlsmeyer I et al. Expression of Potential Targets for Cell-Based Therapies on Melanoma Cells Life (Basel, Switzerland) 2021-03-24 [PMID: 33805080] (IF/IHC, Human)
• Tucci M, D'Oronzo S, Mannavola F et al. Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis Ther Adv Med Oncol 2020-03-13 [PMID: 32206092] (Human)
• Ksander BR, Kolovou PE, Wilson BJ et al. ABCB5 is a limbal stem cell gene required for corneal development and repair. Nature 2014-07-17 [PMID: 25030174] (WB)
• Lee J, Abdeen A, Li Y et al. Gradient and Dynamic Hydrogel Materials to Probe Dynamics in Cancer Stem Cell Phenotypes ACS Applied Bio Materials 2020-12-16
• Roy T, Boateng ST, Banang-Mbeumi S et al. Synthesis, inverse docking-assisted identification and in vitro biological characterization of Flavonol-based analogs of fisetin as c-Kit, CDK2 and mTOR inhibitors against melanoma and non-melanoma skin cancers Bioorganic chemistry 2021-02-01 [PMID: 33450548]

Bioinformatics

• Gene Symbol: ABCB5
• Entrez:
  • 340273(Human)
• Uniprot:
  • Q2M3G0()