ABCB5 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ABCB5 Antibody [NBP1-77687] - ABCB5 antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: ABCB5 Antibody [NBP1-77687] - Analysis of ABCB5 in mouse spleen using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ABCB5 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human ABCB5 protein (between residues 150-200) [UniProt Q2M3G0]
Localization
Cell membrane; Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCB5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This ABCB5 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Western blot reported in scientific literature (PMID: 25030174).
Control
A-431 Whole Cell Lysate
Publications
Read Publications using
NBP1-77687 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCB5 Antibody

  • ABCB5 P-gp
  • ABCB5
  • ABCB5alpha
  • ABCB5beta
  • ATP-binding cassette protein
  • ATP-binding cassette sub-family B member 5
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • EC 3.6.3
  • EC 3.6.3.44
  • EST422562
  • P-glycoprotein ABCB5

Background

The ATP-binding cassette sub-family B member 5 (ABCB5) is an ABC transporter and PGP member. It is highly expressed in skin and in malignant melanoma. When two abnormal growths of tissues occur, resulting in multiple malignancies, ABCB5 is often highly expressed. This elevated expression in tumor cells can indicate a propensity towards therapeutic failure in many cases. Analysis of ABCB5 concentrations in tumors has been suggested as a fairly dependable qualitative biomarker for assessing the malignancy of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ABCB5 Antibody (NBP1-77687)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ABCB5 Antibody (NBP1-77687) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77687AF350
Alexa Fluor 405 NBP1-77687AF405
Alexa Fluor 488 NBP1-77687AF488
Alexa Fluor 532 NBP1-77687AF532
Alexa Fluor 594 NBP1-77687AF594
Alexa Fluor 647 NBP1-77687AF647
Alexa Fluor 700 NBP1-77687AF700
Alexa Fluor 750 NBP1-77687AF750
Biotin NBP1-77687B
DyLight 350 NBP1-77687UV
DyLight 405 NBP1-77687V
DyLight 488 NBP1-77687G
DyLight 550 NBP1-77687R
DyLight 594 NBP1-77687DL594
DyLight 650 NBP1-77687C
DyLight 680 NBP1-77687FR
DyLight 755 NBP1-77687IR
FITC NBP1-77687F
Janelia Fluor 549 NBP1-77687JF549
Janelia Fluor 646 NBP1-77687JF646

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Blogs on ABCB5

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol ABCB5
Entrez
Uniprot