ABCF2 Antibody

Images

 
Western Blot: ABCF2 Antibody [NB400-115] - Analysis of ABCF2 on Lane 1: HeLa whole cell extracts and Lane 2: NIH/3T3 cell lysates using NB400-115.
Immunocytochemistry/ Immunofluorescence: ABCF2 Antibody [NB400-115] - ABCF2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: ABCF2 Antibody [NB400-115] - Simple Western lane view shows a specific band for ABCF2 in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, YeSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ABCF2 Antibody Summary

Immunogen
A fusion protein containing amino acids 1-102 of the ABCF2 protein.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ABCF2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This ABCF2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot where a band is observed at approx. 71 kDa. In ICC/IF cytoplasmic staining was observed.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Yeast ABCF2.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for ABCF2 Antibody

  • ABC28
  • ABC-type transport protein
  • ATP-binding cassette sub-family F member 2
  • ATP-binding cassette, sub-family F (GCN20), member 2
  • DKFZp586K1823
  • EC 3.6.3
  • EC 3.6.3.17
  • EST133090
  • HUSSY18
  • HUSSY-18
  • Iron inhibited ABC transporter 2
  • Iron-inhibited ABC transporter 2
  • M-ABC1

Background

ABCF2 is a member of the ABC Transporter superfamily and of the GCN20 subfamily. As a member of the ABC Transporter family, it functions to transport various molecules across extra- and intracellular membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for ABCF2 Antibody (NB400-115): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ABCF2 Antibody (NB400-115)

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Blogs on ABCF2.

Cancer studies with ABCF2
ATP-binding cassette superfamily F2 (ABCF2) is a member of the ATP-binding cassette (ABC) transporter superfamily, and more specifically, a member of the GCN20 subfamily. Most members of this family are membrane proteins that transport various substra...  Read full blog post.

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Bioinformatics

Gene Symbol ABCF2
Entrez
Uniprot