eRF1 Antibody (4F9H12) - BSA Free

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 and CC-90009 on COL17A1 PTC readthrough. JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-90009 for 72 h and eRF3a, eRF3b and eRF1 (NBP2-52552) levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33764477/) licensed under a CC-BY license.

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against HEK293 (1) and RF1 (AA: 288-437)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using eRF1 mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).

ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 & CC-90009 on COL17A1 PTC readthrough. (A, B) HaCaT keratinocytes derived from an unaffected individual were exposed to various concentrations of CC-885 (A) or CC-90009 (B) with or without 100 μg/ml gentamicin sulfate for 48 h & cell viability was measured using the MTT assay in triplicate samples (±SD). Concentrations of CC-885 & CC-90009 are in nanomolar. (C, D) JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-885 (C) or CC-90009 (D) for 72 h & eRF3a, eRF3b & eRF1 levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. (E, F) JEB01 keratinocytes were exposed to the indicated concentrations of CC-885 (E) or CC-90009 (F) in the presence or absence of 100 or 300 μg/ml gentamicin sulfate for 72 h & Collagen XVII levels were measured by SDS-PAGE followed by traditional western blotting. beta -actin was used as loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33764477), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, Flow, CyTOF-ready
• Clone: 4F9H12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

eRF1 Antibody (4F9H12) - BSA Free Summary

• Description: Novus Biologicals Mouse eRF1 Antibody (4F9H12) - BSA Free (NBP2-52552) is a monoclonal antibody validated for use in WB, ELISA, Flow and Simple Western. Anti-eRF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human eRF1 (AA: 288-437) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ETF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Simple Western 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
  See Simple Western Antibody Database for Simple Western validation: Tested in Caov-3, GM00798, HCT 116, HDQ-P1, NCI-H1299, SW900, separated by Size, antibody dilution of 1:100
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33764477).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for eRF1 Antibody (4F9H12) - BSA Free

• D5S1995
• ERF1eRF1
• eukaryotic peptide chain release factor subunit 1
• Eukaryotic release factor 1
• eukaryotic translation termination factor 1
• polypeptide chain release factor 1
• Protein Cl1
• RF1ERF
• sup45 (yeast omnipotent suppressor 45) homolog-like 1
• SUP45L1
• TB3-1MGC111066

Background

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eRF1 Antibody (NBP2-52552)(1)

Publication using NBP2-52552

• Baradaran-Heravi A, Balgi AD, Hosseini-Farahabadi S et al. Effect of small molecule eRF3 degraders on premature termination codon readthrough Nucleic acids research 2021-03-25 [PMID: 33764477] (WB, Human)

Bioinformatics

• Gene Symbol: ETF1