A20/TNFAIP3 Antibody (775928) [Unconjugated]

A20/TNFAIP3 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal breast using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal liver using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 775928
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

A20/TNFAIP3 Antibody (775928) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human A20/TNFAIP3
  Lys91-Leu263
  Accession # P21580
• Specificity: Detects human A20/TNFAIP3 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFAIP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A20/TNFAIP3 Antibody (775928) [Unconjugated]

• A20
• A20TNFA1P2
• EC 3.4.19.12
• EC 6.3.2.-
• MGC104522
• MGC138687
• OTU domain-containing protein 7C
• OTUD7C
• OTUD7CMGC138688
• Putative DNA-binding protein A20
• TNF alpha-induced protein 3
• TNFA1P2
• TNFAIP3
• tumor necrosis factor alpha-induced protein 3
• tumor necrosis factor inducible protein A20
• tumor necrosis factor, alpha-induced protein 3
• Zinc finger protein A20

Background

A20, also called TNF alpha -induced protein 3 (TNFAIP3), is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for A20/TNFAIP3 Antibody (MAB75981). (Showing 1 - 1 of 1 FAQ).

1. Following cleavage of A20/TNFAIP3, two full length fragments are released, one with 37 kDa and other one was approx. at 52 kDa. There are mentioning of 75Kda fragment and 37 KDa fragment in scientific papers but not 52 KDa. What is the significance of that specific band?
   • There is data available that explains 52 KDa band in A20/TNFAIP3. Using this antibody in Western Blot will result multiple bands. In LPS treatment, 52 KDa putative cleavage band was observed; however, the same band was not found in untreated THP1 cells.

Bioinformatics

• Gene Symbol: TNFAIP3
• Uniprot:
  • P21580()