5T4 Antibody

Western Blot: 5T4 Antibody [NBP1-90315] - Analysis in control (vector only transfected HEK293T lysate) and TPBG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: 5T4 Antibody [NBP1-90315] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: 5T4 Antibody [NBP1-90315] - Analysis in human seminal vesicle and liver tissues. Corresponding 5T4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: 5T4 Antibody [NBP1-90315] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: 5T4 Antibody [NBP1-90315] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: 5T4 Antibody [NBP1-90315] - Staining of human liver shows weak membranous positivity in hepatocytes as expected.

Immunohistochemistry: 5T4 Antibody [NBP1-90315] - Staining of human seminal vesicle shows strong membranous positivity in glandular cells.

Product Discontinued

5T4 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPBG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for 5T4 Antibody

• 5T4 oncofetal trophoblast glycoprotein
• 5T4 oncotrophoblast glycoprotein
• 5T4
• 5T4AG
• 5T4-AG
• 5T4M6P1,5T4 oncofetal antigen
• M6P1
• TPBG
• trophoblast glycoprotein

Background

The 5T4 gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is ass

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPBG
• Entrez:
  • 7162(Human)
• Uniprot:
  • Q13641()