SPRY4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY4 at approximately 45 kDa (as indicated) using 10 µg/mL of Sheep ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SPRY4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SPRY4
Met1-His178
Accession # NP_112226
Specificity
Detects endogenous human SPRY4 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SPRY4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5070 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPRY4 Antibody [Unconjugated]

  • protein sprouty homolog 4
  • sprouty homolog 4 (Drosophila)
  • SPRY4
  • spry-4

Background

SPRY4 (sprouty 4) is a member of the sprouty family of proteins. It blocks tyrosine kinase growth factor activation of the MAPK signaling pathway, possibly through an interaction with either Raf1 or TESK1. Human SPRY4 is 322 amino acids (aa) in length. It contains three SH3-binding domains (aa 3‑9; 25‑31; 168‑174), an NLS (aa 87‑93), a PEST sequence (aa 118‑133), and a Cys-rich/Zn++-finger domain that binds both TESK1 and Raf1 (aa 182‑298). There are two variant isoforms. One is a short form that shows an alternate start site at Met24, while a second shows a 10 aa substitution for aa 120‑129, followed by a premature truncation. Over aa 1‑177, human SPRY4 is 89% aa identical to canine SPRY4. Mouse SPRY4 is orthologous to the human short form, and shares 90% aa identity with human SPRY4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPRY4 Antibody (AF5070)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPRY4
Uniprot