SPRY4 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY4 at approximately 45 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

KSRP is a novel regulator of Spry4.A and B, total RNA from H2122 and H157 cells treated with KSRP-specific siRNAs was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” **, p < 0.01, t test. C, total RNA from Beas2B cells transiently transfected with either control or FLAG-KSRP expression vectors was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” *, p < 0.05, t test. D, total RNA from H2122 clones with stable expression of control shRNA or KSRP shRNA was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” *, p < 0.05; **, p < 0.01, ANOVA. E and F, proteins from H2122 and H157 cells treated with KSRP-specific siRNAs were isolated, and the expression of Spry4 was later determined by immunoblotting. Representative data of two independent, highly reproducible experiments are displayed. Ctrl, control; IB, immunoblotting. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0021925820428558), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SPRY4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SPRY4
  Met1-His178
  Accession # NP_112226
• Specificity: Detects endogenous human SPRY4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SPRY4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPRY4 Antibody [Unconjugated]

• protein sprouty homolog 4
• sprouty homolog 4 (Drosophila)
• SPRY4
• spry-4

Background

SPRY4 (sprouty 4) is a member of the sprouty family of proteins. It blocks tyrosine kinase growth factor activation of the MAPK signaling pathway, possibly through an interaction with either Raf1 or TESK1. Human SPRY4 is 322 amino acids (aa) in length. It contains three SH3-binding domains (aa 3‑9; 25‑31; 168‑174), an NLS (aa 87‑93), a PEST sequence (aa 118‑133), and a Cys-rich/Zn⁺⁺-finger domain that binds both TESK1 and Raf1 (aa 182‑298). There are two variant isoforms. One is a short form that shows an alternate start site at Met24, while a second shows a 10 aa substitution for aa 120‑129, followed by a premature truncation. Over aa 1‑177, human SPRY4 is 89% aa identical to canine SPRY4. Mouse SPRY4 is orthologous to the human short form, and shares 90% aa identity with human SPRY4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPRY4 Antibody (AF5070)(2)

Publications using AF5070

• Hengyu Lu, Chen Liu, Hung Huynh, Thi Bich Uyen Le, Matthew J. LaMarche, Morvarid Mohseni et al. Resistance to allosteric SHP2 inhibition in FGFR-driven cancers through rapid feedback activation of FGFR Oncotarget 1/21/2020 [PMID: 32076487]
• RK Bikkavilli, SA Zerayesus, M Van Scoyk, L Wilson, PY Wu, A Baskaran, K Tang, S Raheem, BA Samuelson, NM Reddy, SP Reddy, CD Cool, B Kosmider, S Avasarala, RA Winn KSRP promotes post-transcriptional destabilization of Spry4 transcripts in non-small cell lung cancer J. Biol. Chem, 2017-03-08;0(0):. 2017-03-08 [PMID: 28275056] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SPRY4
• Uniprot:
  • NP_112226()