Glypican 6 Antibody [Unconjugated]

GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A. Image collected and cropped by CiteAb from the following open publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

GDE2 substrates show impaired release in SOD1G93A mice. a Schematic of the sequential solubilization protocol used to separate hydrophilic non-membrane proteins (S1) from hydrophobic membrane proteins (S2). b Western blot demonstrating the successful enrichment of non-membrane protein (GAPDH) in the S1 fraction and membrane protein (Na/K ATPase) in the S2 fraction for WT and SOD1G93A transgenic mice. c Western blots visualizing the impaired production of non-membrane bound (S1) Glypican 6, Glypican 4, and Glypican 1 in the lumbar spinal cord of 4 month SOD1G93A mice. d Quantification of the reduced membrane release of Glypican 6, *p = 0.038; Glypican 4, *p = 0.016; and Glypican 1, *p = 0.030 relative to GAPDH. Graph represents mean ± SEM. Student’s t test, n = 4 WT, 4 SOD1G93A Image collected and cropped by CiteAb from the following open publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-017-0148-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 6 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Glypican 6
  Asp24-Val513
  Accession # Q9R087
• Specificity: Detects mouse Glypican 6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPC6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 6 Antibody [Unconjugated]

• Glypican 6
• glypican proteoglycan 6
• glypican-6
• GPC6
• MGC126288
• OMIMD1

Background

Glypican 6 (glypiated proteoglycan 6; also GPC6) is an 85-95 kDa heparan sulfate proteoglycan member of the GPI-linked glypican family of molecules. It is expressed by postnatal chrondrocytes and embryonic mesenchyme (connective tissue plus smooth muscle cells), and potentially modulates the availability, or signaling, of heparin-binding growth factors. Mouse Glypican 6 is synthesized as a 532 amino acid (aa) proprecursor that contains a 507 mature region (aa 4-530) and a 25 aa C-terminal prosegment. There is one potential splice variant that shows a 10 aa insertion after Leu384. Over aa 4-513, mouse Glypican 6 shares 99% aa identity with both human and rat Glypican 6.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 6 Antibody (AF1053)(3)

Publications using AF1053

• Meyer, J;Yu, K;Luna-Figueroa, E;Deneen, B;Noebels, J; Glioblastoma disrupts cortical network activity at multiple spatial and temporal scales Nature communications 2024-05-27 [PMID: 38802334] (Immunohistochemistry, Human)
• Niebergall, EB;Weekley, D;Mazur, A;Olszewski, NA;DeSchepper, KM;Radant, N;Vijay, AS;Risher, WC; Abnormal Morphology and Synaptogenic Signaling in Astrocytes Following Prenatal Opioid Exposure Cells 2024-05-14 [PMID: 38786059] (Western Blot, Rat)
• C Cave, S Park, M Rodriguez, M Nakamura, A Hoke, M Pletnikov, S Sockanatha GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord Mol Neurodegener, 2017-01-19;12(1):8. 2017-01-19 [PMID: 28103900] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GPC6
• Uniprot:
  • Q9R087()