Glypican 6 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Glypican 6 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Glypican 6
  Asp24-Val513
  Accession # Q9R087
• Specificity: Detects mouse Glypican 6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPC6
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 6 Antibody [Unconjugated]

• Glypican 6
• glypican proteoglycan 6
• glypican-6
• GPC6
• MGC126288
• OMIMD1

Background

Glypican 6 (glypiated proteoglycan 6; also GPC6) is an 85-95 kDa heparan sulfate proteoglycan member of the GPI-linked glypican family of molecules. It is expressed by postnatal chrondrocytes and embryonic mesenchyme (connective tissue plus smooth muscle cells), and potentially modulates the availability, or signaling, of heparin-binding growth factors. Mouse Glypican 6 is synthesized as a 532 amino acid (aa) proprecursor that contains a 507 mature region (aa 4-530) and a 25 aa C-terminal prosegment. There is one potential splice variant that shows a 10 aa insertion after Leu384. Over aa 4-513, mouse Glypican 6 shares 99% aa identity with both human and rat Glypican 6.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 6 Antibody (AF1053)(1)

Publication using AF1053

• C Cave, S Park, M Rodriguez, M Nakamura, A Hoke, M Pletnikov, S Sockanatha GDE2 is essential for neuronal survival in the postnatal mammalian spinal cord Mol Neurodegener, 2017;12(1):8. 2017 [PMID: 28103900] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GPC6
• Uniprot:
  • Q9R087()