5'-Nucleotidase/CD73 Antibody (AD2)


Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were ...read more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, IHC, IHC-Fr
1.0 mg/ml

Order Details

5'-Nucleotidase/CD73 Antibody (AD2) Summary

Human CD73 [UniProt# P21589]
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Cell Surface)
  • Flow Cytometry 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 1989648).
Read Publications using
NBP2-48480 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (AD2)

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • ad2 anti cd73
  • ad2 cd73
  • CD73 antigen
  • CD73
  • E5NT
  • EC
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase


This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (NBP2-48480)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, Flow, IHC-Fr.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-48480 Applications Species
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Human)

This citation used the Allophycocyanin form of this antbody
Flow Human
Mulligan SP. Human B cells: differentiation and neoplasia. Leuk Lymphoma. 1990 [PMID: 20394556]
Campeau PM, Rafei M, Boivin MN et al. Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome. Blood. 2009 Oct 8 [PMID: 19587377] (FLOW, Human) FLOW Human
Borrione P, Peola S, Mariani S et al. CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol. 1999 May 15 [PMID: 10229870] (FLOW, Human) FLOW Human
Resta R, Hooker SW, Laurent AB et al. Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto-5'-nucleotidase (CD73). J Clin Invest. 1997 Feb 15 [PMID: 9045870] (FLOW, Human)

5'-Nucleotidase/CD73 antibody (clone AD2) was used for immunofluorescence staining /FLOW analysis of single cell suspensions of thymocytes, splenocytes, lymph node cells, and peripheral blood mononuclear cells from lck(NT) transgenic mouse (mice overexpressing 5'-NT/CD73 in cortical thymocytes under the control of the lck proximal promoter).
FLOW Human
Sidorenko SP, Clark EA. Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes. J Immunol. 1993 Nov 1 [PMID: 8409422]
Nakamura T, Kubagawa H, Ohno T, Cooper MD. Characterization of an IgM Fc-binding receptor on human T cells. J Immunol. 1993 Dec 15 [PMID: 8258701] (FLOW, Human) FLOW Human
Gutensohn W, Resta R, Misumi Y et al. Ecto-5'-nucleotidase activity is not required for T cell activation through CD73. Cell Immunol. 1995 Apr 1 [PMID: 7697732] (FLOW, Human)

5'-Nucleotidase/CD73 antibody (clone AD2) was used for FLOW analysis of Jurkat cells transfectants - Control, Empty vector, Wild type CD73, mutant CD73 His92 to Ala , and mutant CD73 His194 to Ala (Figure 1).
FLOW Human
Patel DD, Whichard LP, Radcliff G et al. Characterization of human thymic epithelial cell surface antigens: phenotypic similarity of thymic epithelial cells to epidermal keratinocytes. J Clin Immunol. 1995 Mar 1 [PMID: 7559912] (FLOW, Human) FLOW Human
Clark EA, Lanier LL. Report from Vienna: in search of all surface molecules expressed on human leukocytes. J Clin Immunol. 1989 Jul 1 [PMID: 2788657]
Show All 15 Publications.

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FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-48480). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-48480AF350
Alexa Fluor 405 NBP2-48480AF405
Alexa Fluor 488 NBP2-48480AF488
Alexa Fluor 532 NBP2-48480AF532
Alexa Fluor 594 NBP2-48480AF594
Alexa Fluor 647 NBP2-48480AF647
Alexa Fluor 700 NBP2-48480AF700
Alexa Fluor 750 NBP2-48480AF750
Allophycocyanin NBP2-48480APC
Biotin NBP2-48480B
DyLight 350 NBP2-48480UV
DyLight 405 NBP2-48480V
DyLight 488 NBP2-48480G
DyLight 550 NBP2-48480R
DyLight 594 NBP2-48480DL594
DyLight 650 NBP2-48480C
DyLight 680 NBP2-48480FR
DyLight 755 NBP2-48480IR
FITC NBP2-48480F
HRP NBP2-48480H
Janelia Fluor 549 NBP2-48480JF549
Janelia Fluor 646 NBP2-48480JF646
PE NBP2-48480PE
PerCP NBP2-48480PCP

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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Gene Symbol NT5E