5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Images

 
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the ...read more
Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control ...read more
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, IHC, IHC-Fr, IHC-P
Clone
AD2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free Summary

Immunogen
Human CD73 [UniProt# P21589]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 1989648). 5'-Nucleotidase/CD73 Antibody (AD2) validated for Immunohistochemistry-Paraffin from a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-48480 in the following applications:

Publications
Read Publications using
NBP2-48480 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'-Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • ad2 anti cd73
  • ad2 cd73
  • CD73 antigen
  • CD73
  • E5NT
  • EC 3.1.3.5
  • ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase

Background

This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 5'-Nucleotidase/CD73 Antibody (NBP2-48480)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, Flow, IHC-Fr.


Filter By Application
FLOW
(6)
Flow
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-48480 Applications Species
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Human)

Details:
This citation used the Allophycocyanin form of this antbody
Flow Human
Mulligan SP. Human B cells: differentiation and neoplasia. Leuk Lymphoma. 1990 [PMID: 20394556]
Campeau PM, Rafei M, Boivin MN et al. Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome. Blood. 2009 Oct 8 [PMID: 19587377] (FLOW, Human) FLOW Human
Borrione P, Peola S, Mariani S et al. CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol. 1999 May 15 [PMID: 10229870] (FLOW, Human) FLOW Human
Resta R, Hooker SW, Laurent AB et al. Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto-5'-nucleotidase (CD73). J Clin Invest. 1997 Feb 15 [PMID: 9045870] (FLOW, Human)

Details:
5'-Nucleotidase/CD73 antibody (clone AD2) was used for immunofluorescence staining /FLOW analysis of single cell suspensions of thymocytes, splenocytes, lymph node cells, and peripheral blood mononuclear cells from lck(NT) transgenic mouse (mice overexpressing 5'-NT/CD73 in cortical thymocytes under the control of the lck proximal promoter).
FLOW Human
Sidorenko SP, Clark EA. Characterization of a cell surface glycoprotein IPO-3, expressed on activated human B and T lymphocytes. J Immunol. 1993 Nov 1 [PMID: 8409422]
Nakamura T, Kubagawa H, Ohno T, Cooper MD. Characterization of an IgM Fc-binding receptor on human T cells. J Immunol. 1993 Dec 15 [PMID: 8258701] (FLOW, Human) FLOW Human
Gutensohn W, Resta R, Misumi Y et al. Ecto-5'-nucleotidase activity is not required for T cell activation through CD73. Cell Immunol. 1995 Apr 1 [PMID: 7697732] (FLOW, Human)

Details:
5'-Nucleotidase/CD73 antibody (clone AD2) was used for FLOW analysis of Jurkat cells transfectants - Control, Empty vector, Wild type CD73, mutant CD73 His92 to Ala , and mutant CD73 His194 to Ala (Figure 1).
FLOW Human
Patel DD, Whichard LP, Radcliff G et al. Characterization of human thymic epithelial cell surface antigens: phenotypic similarity of thymic epithelial cells to epidermal keratinocytes. J Clin Immunol. 1995 Mar 1 [PMID: 7559912] (FLOW, Human) FLOW Human
Clark EA, Lanier LL. Report from Vienna: in search of all surface molecules expressed on human leukocytes. J Clin Immunol. 1989 Jul 1 [PMID: 2788657]
Show All 15 Publications.

Review for 5'-Nucleotidase/CD73 Antibody (NBP2-48480) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-48480:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin 5'-Nucleotidase/CD73 NBP2-48480
Enlarge
5
reviewed by:
Sarah Miller
IHC-P Human 01/19/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLung tissue
SpeciesHuman
LotAD2

Comments

CommentsPFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution).
***Novus Innovators Program - new species or application used on a primary antibody.***

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FAQs for 5'-Nucleotidase/CD73 Antibody (NBP2-48480). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

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Blogs on 5'-Nucleotidase/CD73.

Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target
By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

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Recent Reviews

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Sarah Miller
01/19/2022
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol NT5E