5T4 Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

5T4 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse 5T4
  Ser30-Ser361
  Accession # AAH58198
• Specificity: Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human 5T4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 5T4 Antibody [Alexa Fluor® 750]

• 5T4 oncofetal trophoblast glycoprotein
• 5T4 oncotrophoblast glycoprotein
• 5T4
• 5T4AG
• 5T4-AG
• 5T4M6P1,5T4 oncofetal antigen
• M6P1
• TPBG
• trophoblast glycoprotein

Background

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72‑80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30‑361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90‑338). Over aa 30‑361, mouse 5T4 shares 95% and 81% aa identity with rat and human 5T4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.