5'-Nucleotidase/CD73 Antibody


Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Paraffin-embedded MDAMB231 xenograft, using antibody at 1:100 dilution.

Product Details

Reactivity Hu, BovineSpecies Glossary
Applications WB, IHC, IHC-P
0.97 mg/ml

Order Details

5'-Nucleotidase/CD73 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 211 and 538 of CD73 (Uniprot ID#P21589)
Cell membrane, GPI-anchor
Predicted Species
Bovine (91%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
0.01% Thimerosal
0.97 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
For IHC-P, use suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Reactivity Notes

Human. Predicted cross-reactivity based on sequence identity: Mouse (88%), Rat (88%), Bovine (91%).

Alternate Names for 5'-Nucleotidase/CD73 Antibody

  • 5' nucleotidase (CD73)
  • 5'-NT
  • 5-NT
  • 5'Nucleotidase
  • 5'-nucleotidase, ecto (CD73)
  • CD73 antigen
  • CD73
  • E5NT
  • EC
  • Ecto-5'-nucleotidase
  • eN
  • eNT
  • NT
  • NT55'-nucleotidase
  • NT5E
  • NTE
  • Purine 5-Prime-Nucleotidase


Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, GS
Species: Hu, Po, Ca
Applications: WB, Flow, IHC, ICC
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu, Bovine
Applications: WB, IHC, IHC-P

Publications for 5'-Nucleotidase/CD73 Antibody (NBP1-32951) (0)

There are no publications for 5'-Nucleotidase/CD73 Antibody (NBP1-32951).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 5'-Nucleotidase/CD73 Antibody (NBP1-32951) (0)

There are no reviews for 5'-Nucleotidase/CD73 Antibody (NBP1-32951). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for 5'-Nucleotidase/CD73 Antibody (NBP1-32951). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies


Isotype Controls

Additional 5'-Nucleotidase/CD73 Antibody Products

Related Products by Gene

Bioinformatics Tool for 5'-Nucleotidase/CD73 Antibody (NBP1-32951)

Discover related pathways, diseases and genes to 5'-Nucleotidase/CD73 Antibody (NBP1-32951). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 5'-Nucleotidase/CD73 Antibody (NBP1-32951)

Discover more about diseases related to 5'-Nucleotidase/CD73 Antibody (NBP1-32951).

Pathways for 5'-Nucleotidase/CD73 Antibody (NBP1-32951)

View related products by pathway.

PTMs for 5'-Nucleotidase/CD73 Antibody (NBP1-32951)

Learn more about PTMs related to 5'-Nucleotidase/CD73 Antibody (NBP1-32951).

Research Areas for 5'-Nucleotidase/CD73 Antibody (NBP1-32951)

Find related products by research area.

Blogs on 5'-Nucleotidase/CD73.

CD73 (Cluster of differentiation 73, ecto-5'-nucleotidase)
CD73 is a 70kD glycosyl-phosphatidylinositol (GPI)-anchored cell surface molecule that belongs to the 5'-nucleosidase family. It hydrolyzes extracellular nucleotides into membrane permeable nucleosides and is found as both a membrane-bound and soluble...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NT5E

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-32951 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought