5'-Nucleotidase/CD73 Antibodies
We offer 5'-Nucleotidase/CD73 Antibodies for use in common research applications: ELISA, Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each 5'-Nucleotidase/CD73 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 5'-Nucleotidase/CD73 Antibodies can be used in a variety of model species: Equine, Human, Mouse, Porcine, Rat. Use the list below to choose the 5'-Nucleotidase/CD73 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 5'-Nucleotidase/CD73 polyclonal antibodies and browse our 5'-Nucleotidase/CD73 monoclonal antibody catalog.
Alternate Names for 5'-Nucleotidase/CD73 Antibodies
anti-5'-Nucleotidase/CD73 antibody, anti-NT5E antibody, anti-5' nucleotidase (CD73) antibody, anti-5'-NT antibody, anti-5'-nucleotidase, ecto (CD73) antibody, anti-CD73 antibody, anti-CD73 antigen antibody, anti-E5NT antibody, anti-EC 3.1.3.5 antibody, anti-ecto-5'-nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT55'-nucleotidase antibody, anti-NTE antibody, anti-Purine 5-Prime-Nucleotidase antibody
44 Results for "5-nucleotidase-cd73" in Primary Antibodies
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." alt="CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0027.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human liver shows strong membranous positivity in hepatocytes.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human aortic valve staining for CD73. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0019.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining for pig aortic valve. Image from verified customer review.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-NBP1-85740-img0020.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0025.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-85740-img0026.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Immunohistochemistry-Paraffin-NBP2-48480-img0006.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.")
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cytometry-NBP2-48480-img0005.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0001.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0002.jpg)
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-AD2-Flow-Cell-Surface-NBP2-48480-img0004.jpg)
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # ![Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Western-Blot-NBP2-25237-img0004.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) [NBP2-25237] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-45M4F9-Immunohistochemistry-Paraffin-NBP2-25237-img0003.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).](http://images.novusbio.com/fullsize/CD73-Antibody-4G4-Flow-Cytometry-NBP1-60135-img0001.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G4) [NBP1-60135] - Flow cytometric detection of human CD73 on Huvec (mAb 4G4, Cat.# NBP1-60135). Black, red and blue line represents cells only, isotype control and 4G4 (4 ug/ml, respectively).")
![Western Blot: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Western-Blot-NBP2-46259-img0010.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 5'-Nucleotidase/CD73.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0009.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0001.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0002.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0003.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0004.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0005.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0006.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0007.jpg)
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (1G2) [NBP2-46259] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-1G2-Immunohistochemistry-NBP2-46259-img0008.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0005.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - CD73 antibody detects CD73 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: CD73 stained by CD73 antibody diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP2-15815-img0004.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - J5 xenograft, using CD73 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP2-15815-img0003.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5'-Nucleotidase/CD73 antibody [N1N3] (NBP2-15815) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-910202420104432.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP2-15815] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N1N3] diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-15815_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405713.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0006.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Western-Blot-NBP2-37274-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of bladder cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-Immunohistochemistry-NBP2-37274-img0005.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6B10-ELISA-NBP2-37274-img0001.jpg)
