4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

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Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated ...read more
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/CD137. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human ...read more
4‑1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ELISA(Cap), Func, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

4-1BB/TNFRSF9/CD137 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137
Leu24-Gln186
Accession # Q07011
Specificity
Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF9
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Agonist Activity
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human 4-1BB/TNFRSF9/CD137 Antibody (Catalog # AF838)
ELISA Detection: Human 4-1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF838)
Standard: Recombinant Human 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B)
Publications
Read Publications using
AF838 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]

  • 4-1BB ligand receptor
  • 41BB
  • 4-1BB
  • CD137 antigen
  • CD137
  • CD137MGC2172
  • CDw137
  • FLJ43501
  • ILA
  • ILAhomolog of mouse 4-1BB
  • induced by lymphocyte activation (ILA)
  • interleukin-activated receptor, homolog of mouse Ly63
  • receptor protein 4-1BB
  • T cell antigen ILA
  • T-cell antigen 4-1BB homolog
  • T-cell antigen ILA
  • TNFRSF9
  • tumor necrosis factor receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9

Background

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

  1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
  2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 4-1BB/TNFRSF9/CD137 Antibody (AF838)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Functional Assay.


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(1)
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(3)
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Biotin BAF838

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Bioinformatics

Gene Symbol TNFRSF9
Uniprot