Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated ...read more
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/CD137. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human ...read more
4‑1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 ...read more
Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137 Leu24-Gln186 Accession # Q07011
Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for 4-1BB/TNFRSF9/CD137 Antibody [Unconjugated]
4-1BB ligand receptor
ILAhomolog of mouse 4-1BB
induced by lymphocyte activation (ILA)
interleukin-activated receptor, homolog of mouse Ly63
receptor protein 4-1BB
T cell antigen ILA
T-cell antigen 4-1BB homolog
T-cell antigen ILA
tumor necrosis factor receptor superfamily member 9
tumor necrosis factor receptor superfamily, member 9
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.
Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.