4-1BB/TNFRSF9/CD137 Antibody [Biotin]

Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF838, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

4-1BB/TNFRSF9/CD137 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human 4-1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF838) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red ; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

4‑1BB/TNFRSF9/CD137 was detected in fixed Human Peripheral Blood Mononuclear Cells stimulated with PHA and Monensin cells using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF838) at 15 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, Flow, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

4-1BB/TNFRSF9/CD137 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human 4-1BB
  Leu24-Gln186
  Accession # Q07011
• Specificity: Detects human 4‑1BB/TNFRSF9/CD137 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human 4-1BB/TNFRSF9/CD137 Antibody (Catalog # AF838)
  ELISA Detection: Human 4-1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF838)
  Standard: Recombinant Human 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB/TNFRSF9/CD137 Antibody [Biotin]

• 4-1BB ligand receptor
• 41BB
• 4-1BB
• CD137 antigen
• CD137
• CD137MGC2172
• CDw137
• FLJ43501
• ILA
• ILAhomolog of mouse 4-1BB
• induced by lymphocyte activation (ILA)
• interleukin-activated receptor, homolog of mouse Ly63
• receptor protein 4-1BB
• T cell antigen ILA
• T-cell antigen 4-1BB homolog
• T-cell antigen ILA
• TNFRSF9
• tumor necrosis factor receptor superfamily member 9
• tumor necrosis factor receptor superfamily, member 9

Background

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 KD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-1BB/TNFRSF9/CD137 Antibody (BAF838)(1)

Publication using BAF838

• Lindstedt M, Johansson-Lindbom B, Borrebaeck CA Expression of CD137 (4-1BB) on human follicular dendritic cells. Scand. J. Immunol., 2003-04-01;57(4):305-10. 2003-04-01 [PMID: 12662292] (ICC, IHC, Human)

Bioinformatics

• Gene Symbol: TNFRSF9
• Uniprot:
  • Q07011()