Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity
Format
Carrier-Free

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Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to block 4-1BBL-induced IL-2 secretion by mouse T cells. The ED50 for this effect is 0.3-1 µg/mL in the presence of 0.4 µg/mL of recombinant mouse 4-1BBL. Measured by its ability to bind recombinant mouse 4-1BB Ligand in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse 4-1BB/TNFRSF9/CD137 protein
Mouse 4-1BB
(Val24-Leu187)
Accession # P20334
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Val24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfrsf9
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
44.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
62-66 kDa, reducing conditions
Reviewed Applications
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Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

  • 4-1BB ligand receptor
  • 41BB
  • 4-1BB
  • CD137 antigen
  • CD137
  • CD137MGC2172
  • CDw137
  • FLJ43501
  • ILA
  • ILAhomolog of mouse 4-1BB
  • induced by lymphocyte activation (ILA)
  • interleukin-activated receptor, homolog of mouse Ly63
  • receptor protein 4-1BB
  • T cell antigen ILA
  • T-cell antigen 4-1BB homolog
  • T-cell antigen ILA
  • TNFRSF9
  • tumor necrosis factor receptor superfamily member 9
  • tumor necrosis factor receptor superfamily, member 9

Background

4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature mouse 4-1BB consists of a 164 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, mouse 4-1BB shares 60% and 79% aa sequence identity with human and rat 4-1BB, respectively. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cells including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (3-6) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (7-10). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (7, 11). This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-6, 11). It can also enhance the activation-induced cell death of repetitively stimulated T cells (11). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (7, 10) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (8, 9). 4‑1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).
  1. Kwon, B.S. and S.M. Weissman (1989) Proc. Natl. Acad. Sci. USA 86:1963.
  2. Wen, T. et al. (2002) J. Immunol. 168:4897.
  3. Pulle, G. et al. (2006) J. Immunol. 176:2739.
  4. Zheng, G. et al. (2004) J. Immunol. 173:2428.
  5. Kim, D. et al. (2008) J. Immunol. 180:2062.
  6. Lee, S. et al. (2008) Nat. Immunol. 9:917.
  7. Choi, B.K. et al. (2009) J. Immunol. 182:4107.
  8. Nishimoto, H. et al. (2005) Blood 106:4241.
  9. Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
  10. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  11. Lee, S. et al. (2005) J. Immunol. 174:6803.
  12. Ma, B.Y. et al. (2005) Blood 106:2002.
  13. Choi, B.K. et al. (2010) J. Immunol. 185:1404.
  14. Kim, C. et al. (2011) Diabetes 60:3159.
  15. Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
  16. Salih, H.R. et al. (2001) J. Immunol. 167:4059.

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Publications for 4-1BB/TNFRSF9/CD137 (937-4B)(2)

Review for 4-1BB/TNFRSF9/CD137 (937-4B) (1) 51

Average Rating: 5
(Based on 1 review)

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reviewed by:
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12/16/2018
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Summary

LotEJT0318041

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Bioinformatics

Gene Symbol Tnfrsf9
Uniprot