S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse 4‑1BB Ligand/TNFSF9 Arg104-Glu309 Accession # P41274
Detects mouse 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots. In Western blots, approximatley 5% cross‑reactivity with recombinant mouse (rm) Fas Ligand is observed and less than 1% cross-reactivity with rmOX40 Ligand, rmTWEAK, rmTRAIL, and rmTNF-alpha is observed.
Immunogen affinity purified
<0.10 EU per 1 μg of the antibody by the LAL method.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for 4-1BB Ligand/TNFSF9 Antibody [Unconjugated]
homolog of mouse 4-1BB-L
receptor 4-1BB ligand
tumor necrosis factor (ligand) superfamily, member 9
tumor necrosis factor ligand superfamily member 9
4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14‑16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart (1, 2). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines (3). A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases (4). By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and up‑regulates expression of B7-1 and B7-2 costimulatory molecules (3).
Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
Kwon, B., H.W. Lee and B.S Kwon (2002) TRENDS in Immunology 23:378.
Salih, H.R. et al. (2001) J. Immunol. 167:4059.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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