15-PGDH/HPGD Antibody

Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) and patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: x250 (a, b), x100 (c, d). Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY licence.

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its expression. Double-immunofluorescence images show staining for 15-PGDH/HPGD-positive (red) cells and cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, and endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: x400. Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY licence

Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.

Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis. Image courtesy of an anonymous customer review.

Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

15-PGDH/HPGD Antibody Summary

• Immunogen: Purified type I human placental 15-PGDH protein [UniProt# P15428]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPGD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:6000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
• Control:

  15-PGDH/HPGD Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27140190) Human reactivity reported in scientific literature (PMID:33210098).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for 15-PGDH/HPGD Antibody

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase, NAD+ or HPGD) belongs to short-chain dehydrogenases/reductases (SDR) family and is a key enzyme responsible for biological inactivation of prostaglandins as well as related eicosanoids. 15-PGDH has a potential to catalyze NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4, and it brings about prostaglandin inactivation for regulating biological processes which are controlled by prostaglandin levels. It is down-regulated by cortisol, dexamethasone and betamethasone, and TGFB1. 15-PGDH is considered as a tumor suppressor and its decreased expression is observed in cancers subjects with colorectal, breast, prostate, lung, thyroid, gastric and other cancers. Furthermore, defects in 15-PGDH gene have been linked to diseases including hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1), cranioosteoarthropathy (COA), isolated congenital nail clubbing (ICNC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 15-PGDH/HPGD Antibody (NB200-179)(28)

Publications using NB200-179

• Corwin C, Nikolopoulou A, Pan AL et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. J Neuroinflammation Sep 20 2018 [PMID: 30236122] (ICC/IF, Rat)
• Hsiao HM, Thatcher TH, Colas RA et al. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol 2015 Dec [PMID: 26468975]
• Osman J CysLT1 receptor signaling and the tumor microenvironment in colon cancer models Thesis Jan 1 2020
• Volpato M, Cummings M, Shaaban A et al. Downregulation of 15-hydroxyprostaglandin dehydrogenase during acquired tamoxifen resistance and association with poor prognosis in ER alpha-positive breast cancer Exploration of Targeted Anti-tumor Therapy Oct 19 2020 [PMID: 33210098] (Human)
• Yao L, Chen W, Song K et al. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) prevents lipopolysaccharide (LPS)-induced acute liver injury. PLoS ONE. Apr 19 2017 [PMID: 28423012] (IHC-P, Mouse)
• Mehdawi LM, Satapathy SR, Gustafsson A et al. A potential anti-tumor effect of leukotriene C4 through the induction of 15-hydroxyprostaglandin dehydrogenase expression in colon cancer cells. Oncotarget. 2017 May 23 [PMID: 28402256] (ICC/IF, IHC, Human)
• Loo T M, Kamachi F et al. Gut Microbiota Promotes Obesity-Associated Liver Cancer through PGE2-Mediated Suppression of Antitumor Immunity. Cancer Discov Jan 5 2017 [PMID: 28202625] (WB, Mouse)
• Prima V, Kaliberova LN, Kaliberov S et al. COX2/mPGES1/PGE2 pathway regulates PD-L1 expression in tumor-associated macrophages and myeloid-derived suppressor cells Proc. Natl. Acad. Sci. U.S.A Jan 17 2017 [PMID: 28096371] (WB, Mouse)
• Mehdawi LM, Prasad CP, Ehrnstrom R et al. Non-canonical WNT5A signaling up-regulates the expression of the tumor suppressor 15-PGDH and induces differentiation of colon cancer cells. Mol Oncol. Aug 1 2016 [PMID: 27522468] (WB, IHC)
• Miyagishi H, Kosuge Y, Takano A et al. Increased Expression of 15-Hydroxyprostaglandin Dehydrogenase in Spinal Astrocytes During Disease Progression in a Model of Amyotrophic Lateral Sclerosis. Cell. Mol. Neurobiol. May 2 2016 [PMID: 27140190] (WB, IHC-Fr, Mouse)
• de Hair MJ, Leclerc P, Newsum EC et al. Expression of Prostaglandin E2 Enzymes in the Synovium of Arthralgia Patients at Risk of Developing Rheumatoid Arthritis and in Early Arthritis Patients. PLoS ONE. 2015 Aug 03 [PMID: 26225917] (IHC, Human)
• Ryu YM, Myung SJ, Park YS et al. Inhibition of 15-Hydroxyprostaglandin Dehydrogenase by Helicobacter pylori in Human Gastric Carcinogenesis Cancer Prev Res (Phila) 2013 Mar 12 [PMID: 23430757] (WB, Human)
• Kang PS, Kim JH, Moon OI et al. Prognostic implication of 15-hydroxyprostaglandin dehydrogenase down-regulation in patients with colorectal cancer J Korean Soc Coloproctol 2012 Oct [PMID: 23185705] (IHC, Human)
• Subbaramaiah K, Howe LR, Zhou XK et al. Pioglitazone, a PPARgamma Agonist, Suppresses CYP19 Transcription: Evidence for Involvement of 15-Hydroxyprostaglandin Dehydrogenase and BRCA1 Cancer Prev Res 2012 Oct [PMID: 22787115] (WB, Human)
• Gheorghe KR, Sadique S, Leclerc P et al. Limited effect of anti-rheumatic treatment on 15-prostaglandin dehydrogenase in rheumatoid arthritis synovial tissue Arthritis Res Ther 2012 May 22 [PMID: 22616846] (IHC-Fr, ICC/IF, Human)
• Chung HW, Lim JH, Kim MY, Shin SJ, Chung S, Choi BS, Kim HW, Kim YS, Park CW, Chang YS. High-fat diet-induced renal cell apoptosis and oxidative stress in spontaneously hypertensive rat are ameliorated by fenofibrate through the PPAR?-FoxO3a-PGC-1? pathway. Nephrol Dial Transplant. 2011 Nov 9. [PMID: 22076434] (WB, Rat)
• Judson BL, Miyaki A, Kekatpure VD et al. UV radiation inhibits 15-hydroxyprostaglandin dehydrogenase levels in human skin: evidence of transcriptional suppression Cancer Prev Res (Phila) 2010 Sep [PMID: 20643784] (WB, Human)
• Miyaki A, Yang P, Tai H-H et al. Bile acids inhibit NAD+-dependent 15-hydroxyprostaglin dehydrogenase transcription in colonocytes. Am J Physiol Gastrointest Liver Physiol;297(3):G559-566. 2009 [PMID: 19608733]
• Thirumangalakudi L, Rao HV, Grammas P. Involvement of PGE2 and PGDH but not COX-2 in thrombin-induced cortical neuron apoptosis. Neurosci Lett 2009 Mar 13 [PMID: 19383433] (WB, Rat)
• Hughes D, Otani T, Yang P et al. NAD+-dependent 15-hydroxyprostaglandin dehydrogenase regulates levels of bioactive lipids in non-small cell lung cancer. Cancer Prev Res (Phila) 1(4):241-9. 2008 Sep. [PMID: 19138967]
• Backlund MG, Mann JR, Holla VR et al. Repression of 15-hydroxyprostaglandin dehydrogenase involves histone deacetylase 2 and snail in colorectal cancer. Cancer Res 68(22):9331-7. 2008 Nov 15. [PMID: 19010907]
• Michelet JF, Colombe L, Gautier B et al. Expression of NAD+ dependent 15-hydroxyprostaglandin dehydrogenase and protection of prostaglandins in human hair follicle. Exp Dermatol 2008 Oct [PMID: 18328086] (WB, Human)
• Mann, JR et al. Repression of prostaglandin dehydrogenase by epidermal growth factor and snail increases prostaglandin E2 and promotes cancer progression. Cancer Res 66(13):6649-56. 2006 Jul 1. [PMID: 16818638]
• Yan, M et al. 15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers. Proc Natl Acad Sci U S A 101(50):17468-73. 2004 Dec 14. [PMID: 15574495] (WB, IHC, Human)
• Backlund, MG et al. 15-Hydroxyprostaglandin dehydrogenase is down-regulated in colorectal cancer. J Biol Chem 280(5):3217-23. 2005 Feb 4. [PMID: 15542609]
• Ding, Y et al. NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer. Carcinogenesis 26(1):65-72. 2005 Jan. [PMID: 15358636]
• Tong, M, Tai, H-H. Synergistic induction of the nicotinamide adenine dinucleotide-linked 15-hydroxyprostaglandin dehydrogenase by an androgen and interleukin-6 or forskolin in human prostate cancer cells. Endocrinology 145(5):2141-7. 2004 May. [PMID: 14749354]
• Tai, CL et al. Monoclonal antibodies that inhibit the enzyme activity of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. Biochem J 267(1):75-8. 1990 Apr 1. [PMID: 1691637] (Human)

Reviews for 15-PGDH/HPGD Antibody (NB200-179) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Anonymous IF Human 06/07/2018

Summary

• Application: Immunofluorescence
• Sample Tested: Fetal membranes
• Species: Human
• Lot: D-1

reviewed by: James Xiao WB Human 05/28/2014

Summary

• Application: Western Blot
• Sample Tested: Human ling tissue lysates
• Species: Human
• Lot: C-3

Blocking

• Blocking Details: 5% milk in PBST

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% milk/PBST

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG HRP-conjugated
• Secondary Concentration: 1:5000 in blocking buffer

Details

• Detection Notes: Western Lightning Plus ECL from PerkinElmer; 1 mins exposure

reviewed by: Marina Korotkova ICC Human 08/17/2012

Summary

• Application: Immunocytochemistry
• Sample Tested: synovial tissue from patient with rheumatoid arthritis
• Species: Human

Bioinformatics

• Gene Symbol: HPGD