15-PGDH/HPGD Antibody

Images

 
Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing ...read more
Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining ...read more
Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its ...read more
Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.
Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis. Image courtesy of an anonymous customer review.
Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

15-PGDH/HPGD Antibody Summary

Immunogen
Purified type I human placental 15-PGDH protein [UniProt# P15428]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HPGD
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:6000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
Control
15-PGDH/HPGD Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB200-179 in the following applications:

Publications
Read Publications using
NB200-179 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27140190) Human reactivity reported in scientific literature (PMID:33210098).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein A purified

Alternate Names for 15-PGDH/HPGD Antibody

  • 15-hydroxyprostaglandin dehydrogenase [NAD+]
  • 15PGDH
  • 15-PGDH
  • EC 1.1.1
  • EC 1.1.1.141
  • HPGD
  • hydroxyprostaglandin dehydrogenase 15-(NAD)
  • NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
  • PGDH1
  • PGDH1PGDH
  • Prostaglandin dehydrogenase 1
  • SDR36C1
  • short chain dehydrogenase/reductase family 36C, member 1

Background

15-PGDH (15-hydroxyprostaglandin dehydrogenase, NAD+ or HPGD) belongs to short-chain dehydrogenases/reductases (SDR) family and is a key enzyme responsible for biological inactivation of prostaglandins as well as related eicosanoids. 15-PGDH has a potential to catalyze NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4, and it brings about prostaglandin inactivation for regulating biological processes which are controlled by prostaglandin levels. It is down-regulated by cortisol, dexamethasone and betamethasone, and TGFB1. 15-PGDH is considered as a tumor suppressor and its decreased expression is observed in cancers subjects with colorectal, breast, prostate, lung, thyroid, gastric and other cancers. Furthermore, defects in 15-PGDH gene have been linked to diseases including hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1), cranioosteoarthropathy (COA), isolated congenital nail clubbing (ICNC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-1066
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87852
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87311
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-13349
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-59438
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-32550
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85500
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-58268
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00008458-M06
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-87892
Species: Hu, Rt
Applications: WB, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
H00005241-M08
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB200-179
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for 15-PGDH/HPGD Antibody (NB200-179)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(5)
IHC-Fr
(2)
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Human
(11)
Mouse
(4)
Rat
(3)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NB200-179 Applications Species
Corwin C, Nikolopoulou A, Pan AL et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. J Neuroinflammation Sep 20 2018 [PMID: 30236122] (ICC/IF, Rat) ICC/IF Rat
Hsiao HM, Thatcher TH, Colas RA et al. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol 2015 Dec [PMID: 26468975]
Osman J CysLT1 receptor signaling and the tumor microenvironment in colon cancer models Thesis Jan 1 2020
Volpato M, Cummings M, Shaaban A et al. Downregulation of 15-hydroxyprostaglandin dehydrogenase during acquired tamoxifen resistance and association with poor prognosis in ER alpha-positive breast cancer Exploration of Targeted Anti-tumor Therapy Oct 19 2020 [PMID: 33210098] (Human) Human
Yao L, Chen W, Song K et al. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) prevents lipopolysaccharide (LPS)-induced acute liver injury. PLoS ONE. Apr 19 2017 [PMID: 28423012] (IHC-P, Mouse) IHC-P Mouse
Mehdawi LM, Satapathy SR, Gustafsson A et al. A potential anti-tumor effect of leukotriene C4 through the induction of 15-hydroxyprostaglandin dehydrogenase expression in colon cancer cells. Oncotarget. 2017 May 23 [PMID: 28402256] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Loo T M, Kamachi F et al. Gut Microbiota Promotes Obesity-Associated Liver Cancer through PGE2-Mediated Suppression of Antitumor Immunity. Cancer Discov Jan 5 2017 [PMID: 28202625] (WB, Mouse) WB Mouse
Prima V, Kaliberova LN, Kaliberov S et al. COX2/mPGES1/PGE2 pathway regulates PD-L1 expression in tumor-associated macrophages and myeloid-derived suppressor cells Proc. Natl. Acad. Sci. U.S.A Jan 17 2017 [PMID: 28096371] (WB, Mouse) WB Mouse
Mehdawi LM, Prasad CP, Ehrnstrom R et al. Non-canonical WNT5A signaling up-regulates the expression of the tumor suppressor 15-PGDH and induces differentiation of colon cancer cells. Mol Oncol. Aug 1 2016 [PMID: 27522468] (WB, IHC) WB, IHC
Miyagishi H, Kosuge Y, Takano A et al. Increased Expression of 15-Hydroxyprostaglandin Dehydrogenase in Spinal Astrocytes During Disease Progression in a Model of Amyotrophic Lateral Sclerosis. Cell. Mol. Neurobiol. May 2 2016 [PMID: 27140190] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Show All 28 Publications.

Reviews for 15-PGDH/HPGD Antibody (NB200-179) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB200-179:
Filter by Applications
IF
(1)
WB
(1)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence 15-PGDH/HPGD NB200-179
Enlarge
5
reviewed by:
Anonymous
IF Human 06/07/2018
View

Summary

ApplicationImmunofluorescence
Sample TestedFetal membranes
SpeciesHuman
LotD-1
Western Blot 15-PGDH/HPGD NB200-179
Enlarge
5
reviewed by:
James Xiao
WB Human 05/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman ling tissue lysates
SpeciesHuman
LotC-3

Blocking

Blocking Details5% milk in PBST

Primary Anitbody

Dilution Ratio1:1000 in 5% milk/PBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP-conjugated
Secondary Concentration1:5000 in blocking buffer

Details

Detection NotesWestern Lightning Plus ECL from PerkinElmer; 1 mins exposure
Immunocytochemistry 15-PGDH/HPGD NB200-179
Enlarge
4
reviewed by:
Marina Korotkova
ICC Human 08/17/2012
View

Summary

ApplicationImmunocytochemistry
Sample Testedsynovial tissue from patient with rheumatoid arthritis
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 15-PGDH/HPGD Antibody (NB200-179) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB200-179B
HRP NB200-179H

Additional 15-PGDH/HPGD Products

Bioinformatics Tool for 15-PGDH/HPGD Antibody (NB200-179)

Discover related pathways, diseases and genes to 15-PGDH/HPGD Antibody (NB200-179). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 15-PGDH/HPGD Antibody (NB200-179)

Discover more about diseases related to 15-PGDH/HPGD Antibody (NB200-179).
 

Pathways for 15-PGDH/HPGD Antibody (NB200-179)

View related products by pathway.

PTMs for 15-PGDH/HPGD Antibody (NB200-179)

Learn more about PTMs related to 15-PGDH/HPGD Antibody (NB200-179).
 

Research Areas for 15-PGDH/HPGD Antibody (NB200-179)

Find related products by research area.

Blogs on 15-PGDH/HPGD

There are no specific blogs for 15-PGDH/HPGD, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
06/07/2018
Application: IF
Species: Human

James Xiao
05/28/2014
Application: WB
Species: Human

Marina Korotkova
08/17/2012
Application: ICC
Species: Human

Bioinformatics

Gene Symbol HPGD