15-PGDH/HPGD Antibody [HRP]

Western Blot: 15-PGDH Antibody [HRP] [NB200-179H] - Detection of 15-PGDH in LoVo whole cell lysates using NB 200-179. Lane 1 uses a 1:6,000 dilution. Lane 2 uses a 1:1,500 dilution. ECL: 10 second exposure.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

15-PGDH/HPGD Antibody [HRP] Summary

• Immunogen: Purified type I human placental 15-PGDH protein [UniProt# P15428]
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPGD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555
• Control:

  15-PGDH/HPGD Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27140190) Human reactivity reported in scientific literature (PMID:33210098).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for 15-PGDH/HPGD Antibody [HRP]

• 15-hydroxyprostaglandin dehydrogenase [NAD+]
• 15PGDH
• 15-PGDH
• EC 1.1.1
• EC 1.1.1.141
• HPGD
• hydroxyprostaglandin dehydrogenase 15-(NAD)
• NAD+-dependent 15-hydroxyprostaglandin dehydrogenase
• PGDH1
• PGDH1PGDH
• Prostaglandin dehydrogenase 1
• SDR36C1
• short chain dehydrogenase/reductase family 36C, member 1

Background

15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is a novel tumor suppressor in the COX-2 pathway. The protein is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPGD