15-PGDH/HPGD Antibodies
We offer 15-PGDH/HPGD Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each 15-PGDH/HPGD Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 15-PGDH/HPGD Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the 15-PGDH/HPGD Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our 15-PGDH/HPGD polyclonal antibodies and browse our 15-PGDH/HPGD monoclonal antibody catalog.
Alternate Names for 15-PGDH/HPGD Antibodies
anti-15-PGDH/HPGD antibody, anti-HPGD antibody, anti-15-hydroxyprostaglandin dehydrogenase [NAD+] antibody, anti-15-PGDH antibody, anti-EC 1.1.1 antibody, anti-EC 1.1.1.141 antibody, anti-hydroxyprostaglandin dehydrogenase 15-(NAD) antibody, anti-NAD+-dependent 15-hydroxyprostaglandin dehydrogenase antibody, anti-PGDH1PGDH antibody, anti-Prostaglandin dehydrogenase 1 antibody, anti-SDR36C1 antibody, anti-short chain dehydrogenase/reductase family 36C, member 1 antibody
17 Results for "15-pgdh-hpgd" in Primary Antibodies
![Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Simple-Western-NB200-179-img0005.jpg "Simple Western: 15-PGDH/HPGD Antibody [NB200-179] - Simple Western lane view shows a specific band for 15-PGDH/HPGD in 0.5 mg/ml of Lovo (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy and inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) and patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: x250 (a, b), x100 (c, d). Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0015.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH/HPGD) in rheumatoid arthritis synovial tissue and the effects of anti-rheumatic treatment on its expression. Double-immunofluorescence images show staining for 15-PGDH/HPGD-positive (red) cells and cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, and endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: x400. Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3851), licensed under a CC-BY license](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0016.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Western blot analysis of 15-PGDH in Lovo cell lysate.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NB200-179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NB200-179-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB200-179] - IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis . Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB200-179-img0003.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Staining in human fetal membrane tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-NB200-179-img0009.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - A representative western blot and densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, and the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) and 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta-actin to ensure equal loading. Statistical analysis was conducted using an unpaired t-test; *P=0.05, **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/15-PGDH-HPGD Antibody-Knockdown Validated-NB200-179-img0018.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291984.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415384117.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241539722.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Expression of 15-prostaglandin dehydrogenase (15-PGDH) in healthy & inflamed synovial tissue. Immunohistochemical staining reveals positive (brown) staining for 15-PGDH (hematoxilin counterstained) in synovial tissue from healthy individuals (a) & patients with rheumatoid arthritis (b), osteoarthritis (c), or psoriatic arthritis (d). Original magnifications: ×250 (a, b), ×100 (c, d). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202415291976.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-31020241621232.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of LTC4 on 15-PGDH expression in the colon cancer cell line Caco-2(A) Quantification by qPCR of the mRNA expression of 15-PGDH following treatment with 40 nM LTC4 for 48 h in the presence or absence of AP100984 (a CysLT2 receptor antagonist, 1 μM) & JNK inhibitor I (10 μM). (B) Western blot showing 15-PGDH expression (antibody dilution, 1:5000) & densitometric analysis of LTC4-induced 15-PGDH up-regulation before or after the cells were stimulated with 40 nM LTC4 in the presence or absence of AP100984 & JNK inhibitor I for 48 h. (C) Confocal microscopy immunofluorescence images showing the expression of 15-PGDH (in green; antibody dilution, 1:200; DAPI in blue) after 24 h of stimulation with LTC4 in Caco-2 cells. The objective used was 63x. The data are presented as the percent of untreated control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01, ***P<0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205174.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB200-179] - Effect of 15-PGDH down-regulation on the cell differentiation markers SI & Mucin-2(A) A representative western blot & densitometric analysis of LTC4-induced SI protein expression after transfection with a scrambled control siRNA or 15-PGDH siRNA in HT-29 cells are shown. The cells were treated with or without 40 nM LTC4 for 48 h, & the change in the SI protein level was detected using an SI-specific antibody (1:1000 dilution) & 15-PGDH was detected using a 15-PGDH antibody (1:5000 dilution). The membrane was re-probed with an antibody against beta –actin to ensure equal loading. (B) A representative western blot & densitometric analysis performed as in (A) shown here for Caco-2 cells. (C, D) Representative confocal microscopy immunofluorescence images from cells transfected with a scrambled control siRNA or 15-PGDH siRNA with or without stimulation with 40 nM LTC4 for 48 h. The expression of Mucin-2 (in red; antibody dilution, 1:500; DAPI in blue, dilution 1:1000) (C) in HT-29 cells & (D) Caco-2 cells is shown. The objective used was 63x, & the scale bar is 50 μm. The data are presented as the percent of control cells & represent the mean ± SEM of at least three separate experiments. Statistical analysis was conducted using an unpaired t-test; *P≤0.05, **P<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28402256), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416212329.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody [NB200-179] - Cellular localization of 15-prostaglandin dehydrogenase (15-PGDH) in rheumatoid arthritis synovial tissue & the effects of anti-rheumatic treatment on its expression. (a) Double-immunofluorescence images show staining for 15-PGDH-positive (red) cells & cell marker staining (green) for macrophage CD163, fibroblast prolyl-4-hydroxylase, & endothelial cell CD31. Merged images display double-stained cells in yellow. Original magnification: ×400. Light microscopy pictures of representative synovial biopsy sections show immunohistochemical positive (brown) staining for 15-PGDH before & after intra-articular treatment with glucocorticoids (b) & before & 8 weeks after initiation of methotrexate therapy (c) (hematoxilin counterstained). Graphs display the comparative 15-PGDH expression in rheumatoid arthritis synovial tissue before & after treatment with glucocorticoids or methotrexate as a percentage of the positive stained area versus the total tissue area. GC, glucocorticoids; Mtx, methotrexate. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22616846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-179_rabbit-polyclonal-15-pgdh-hpgd-antibody-310202416205129.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human urinary bladder and cerebral cortex tissues using NBP1-87062 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human urinary bladder and cerebral cortex tissues using NBP1-87062 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP1-87062-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis using Anti-HPGD antibody NBP1-87062 (A) shows similar pattern to independent antibody NBP1-87061 (B).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP1-87062-img0015.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human stomach shows moderate to strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0018.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human urinary bladder shows high expression.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0011.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0017.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87062-img0019.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis using Anti-HPGD antibody NBP1-87061 (A) shows similar pattern to independent antibody NBP1-87062 (B).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP1-87061-img0022.jpg "<b>Independent Antibodies Validation. </b>Western Blot: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis using Anti-HPGD antibody NBP1-87061 (A) shows similar pattern to independent antibody NBP1-87062 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87061-img0009.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0021.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis in human cell lines A-549 and A-431 using anti-HPGD antibody. Corresponding HPGD RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP1-87061-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Analysis in human urinary bladder and skeletal muscle tissues. Corresponding HPGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0017.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0018.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0019.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87061] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NBP1-87061-img0020.jpg)
![Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Western-Blot-NBP2-00490-img0004.jpg "Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.")
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00490-img0006.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-15-PGDH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Immunohistochemistry-Paraffin-NBP2-00490-img0005.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0001.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) [NBP2-00490] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-Antibody-2C10-Flow-Cytometry-NBP2-00490-img0002.jpg)
![Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Western-Blot-NBP2-70933-img0002.jpg "Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.")
![Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70933-img0006.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-15-PGDH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0005.jpg)
![Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70933-img0007.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0003.jpg)
![Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-OTI2C10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70933-img0004.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NB100-1482-img0006.jpg "Western Blot: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Liver. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB100-1482-img0007.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NB100-1482] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunohistochemistry-Paraffin-NB100-1482-img0005.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NBP2-21588] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-Western-Blot-NBP2-21588-img0001.jpg "Western Blot: 15-PGDH/HPGD Antibody [NBP2-21588] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [NBP2-21588] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21588-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP2-21588] - Human colon carcinoma, using 15-PGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/15-PGDH-Antibody-Immunohistochemistry-Paraffin-NBP2-21588-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-016-Immunocytochemistry-Immunofluorescence-NBP2-89839-img0001.jpg "Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated , stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-016-Flow-Cytometry-NBP2-89839-img0002.jpg)
![Western Blot: 15-PGDH/HPGD Antibody (101) [NBP2-90625] - Anti-15-PGDH/HPGD rabbit monoclonal antibody at 1:500 dilution. Lane A: LOVO Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-101-Western-Blot-NBP2-90625-img0003.jpg "Western Blot: 15-PGDH/HPGD Antibody (101) [NBP2-90625] - Anti-15-PGDH/HPGD rabbit monoclonal antibody at 1:500 dilution. Lane A: LOVO Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 27 kDa")
![Immunoprecipitation: 15-PGDH/HPGD Antibody (101) [NBP2-90625] - Mouse 15-PGDH/HPGD was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 ul anti-Mouse 15-PGDH/HPGD rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse 15-PGDH/HPGD rabbit monoclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-101-Immunoprecipitation-NBP2-90625-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-027-Immunocytochemistry-Immunofluorescence-NBP2-89841-img0001.jpg "Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: 15-PGDH/HPGD Antibody (027) [NBP2-89841] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated, stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-027-Flow-Cytometry-NBP2-89841-img0002.jpg)
![Western Blot: 15-PGDH/HPGD Antibody [NBP2-98291] - Anti-15-PGDH/HPGD rabbit polyclonal antibody at 1:500 dilution. Lane A: Lovo Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP2-98291-img0003.jpg "Western Blot: 15-PGDH/HPGD Antibody [NBP2-98291] - Anti-15-PGDH/HPGD rabbit polyclonal antibody at 1:500 dilution. Lane A: Lovo Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 15-PGDH/HPGD Antibody [NBP2-98291] - Mouse 15-PGDH/HPGD was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 ul anti-Mouse 15-PGDH/HPGD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse 15-PGDH/HPGD rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunoprecipitation-NBP2-98291-img0004.jpg)