| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22117SS | |||
| Azide and BSA Free | NBP2-81014 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60547SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86847-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1643SS | |||
| Azide and BSA Free | NBP2-80694 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-530SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84830-25ul | |||
![Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody [1A12] (NBP2-22117) at 1 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-22117-img0012.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0009.jpg)
![Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate. Dilution 1:500 - 1:2000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Western-Blot-NBP2-22117-img0008.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0006.jpg)
![Flow Cytometry: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red). Dilution 1:200 - 1:400.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Flow-Cytometry-NBP2-22117-img0004.jpg)
![ELISA: YAP1 Antibody (1A12) [NBP2-22117] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng). Dilution 1:10000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-ELISA-NBP2-22117-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Simple Western: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 20 ug/mL of mouse Anti-YAP1 Monoclonal Antibody (Catalog # NBP2-22117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0011.jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77680-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77680). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77680-img0010.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0007.jpg)
![Western Blot: STIM1 Antibody [NB110-60547] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stim-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Stim-1 Polyclonal Antibody (Catalog # NB110-60547) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stim-1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/STIM1-Antibody-Knockdown-Validated-NB110-60547-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: STIM1 Antibody [NB110-60547] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stim-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Stim-1 Polyclonal Antibody (Catalog # NB110-60547) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stim-1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: STIM1 Antibody [NB110-60547] - Stromal interaction molecule 1 Antibody [NB110-60547] - Detection of STIM1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Stromal-interaction-molecule-1-Antibody-Western-Blot-NB110-60547-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NB110-60547] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-60547-img0005.jpg)
![Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0011.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: MD-2 Antibody [NB100-56655] - Inactivation of MEK-ERK1/2 and NF-kappaB signalling rescues the adverse effects of MD-1 deficiency on Ang II-induced myocyte hypertrophy. Representative western blot of quantitative analysis of the TLR4 and MD-2 protein levels in WT and MD-1-/- mice at 4 weeks after sham or AB surgery (n = 4). *P < 0.05 vs. WT/shams; #P < 0.05 vs. WT/AB. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep41857) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0012.jpg)
![Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.](http://images.novusbio.com/fullsize/MD-2-Antibody-Immunohistochemistry-Paraffin-NB100-56655-img0010.jpg)
![Western Blot: MD-2 Antibody [NB100-56655] - Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-MD2 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: EMP2 Antibody [NBP1-86847] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86847-img0008.jpg "Immunocytochemistry/Immunofluorescence: EMP2 Antibody [NBP1-86847] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0015.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human skin shows moderate to strong positivity in plasma membrane in keratinocytes.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0012.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0013.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human lung shows moderate to strong cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0016.jpg)
![Western Blot: EMP2 Antibody [NBP1-86847] - Analysis of EMP2 in NIH/3T3 transfectant using anti-EMP2 antibody (1:500 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/EMP2-Antibody-Western-Blot-NBP1-86847-img0005.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0008.jpg "Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP1-78427-img0003.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0007.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0009.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 in A-431 cells.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5G11-Immunocytochemistry-Immunofluorescence-NB100-1643-img0010.jpg "Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 in A-431 cells.")
![Immunohistochemistry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 on human skin cancer. NB100-1643 was used at a dilution of 1:200.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5G11-Immunohistochemistry-NB100-1643-img0011.jpg)
![Flow Cytometry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5G11-Flow-Cytometry-NB100-1643-img0008.jpg)
![Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Western-Blot-NB100-530-img0009.jpg "Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF R2/KDR/Flk-1 chimera transfected lysate (20 ug) using NB100-530 (0.5 ug/mL). ECL detection 10 seconds.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Immunohistochemical analyses of VEGFR2, Ang-2, vWF and CD34 in the peri-infarct area of the ischemic brain. Representative merged images counterstained with DAPI (nuclear staining) (blue). Ang-2, VEGFR2, vWF and CD34 antibodies are represented by the green color (arrows). Image collected and cropped by Citeab from the following publication (A Short Bout of Exercise Prior to Stroke Improves Functional Outcomes by Enhancing Angiogenesis. Neuromolecular Med (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-530-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - V6.5 mouse embryonic stem cells (cat# NBP1-41162) stained with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-530-img0008.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of monoclonal antibody to SAV1 on HeLa cell. Antibody concentration 60 ug/ml.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunocytochemistry-Immunofluorescence-H00060485-M02-img0006.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - SAV1 monoclonal antibody (M02), clone 3B2. Analysis of SAV1 expression in HepG2.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Western-Blot-H00060485-M02-img0009.jpg)
![Immunoprecipitation: SAV1 Antibody (3B2) [H00060485-M02] - Analysis of SAV1 transfected lysate using anti-SAV1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SAV1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-Immunoprecipitation-H00060485-M02-img0007.jpg)
![ELISA: SAV1 Antibody (3B2) [H00060485-M02] - Detection limit for recombinant GST tagged SAV1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAV1-Antibody-3B2-ELISA-H00060485-M02-img0011.jpg)
![Western Blot: SAV1 Antibody (3B2) [H00060485-M02] - MST2 & SAV1 increase the levels of PPAR gamma by increasing its protein stability.(A) Increasing amounts (0.5, 1, 3 µg) of Myc-SAV1 were co-expressed with Flag-PPAR gamma and/or HA-MST2 in 293 cells. At 48 h after transfection, cell lysates were immunoblotted with the indicated antibodies. (B) Flag-tagged PPAR gamma or PPAR gamma (128–505) was co-expressed with HA-MST2, HA-MST2-KD (inactive mutant), Myc-SAV1, and/or Myc-SAV1(1–201) in 293 cells as indicated. Cell lysates were prepared at various times after treatment with 40 µg/mL cycloheximide & then immunoblotted with an anti-Flag antibody. The PPAR gamma protein expression was quantified & the half-life of PPAR gamma protein was calculated. Expression of GAPDH was detected as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22292086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00060485-m02_mouse-monoclonal-sav1-antibody-3b2-310202415371988.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84830-img0003.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human placenta shows moderate membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0008.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human colon shows weak to moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0005.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0006.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human lung shows weak to moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0007.jpg)
![Western Blot: Ephrin-B2 Antibody [NBP1-84830] - Colonic levels of EphB4 & ephrin-B2 mRNA. (A) Representative agarose gels showing mRNA levels of EphB4, ephrin-B2 & GAPDH from the colon of vehicle-treated normal mice (N; n = 7) & of TNBS-treated mice administered with vehicle (CNT; n = 4) or EphB4 20 µg/kg (EphB; n = 5). Histograms represent the quantification of EphB4 (B) & ephrin-B2 (C) mRNA levels normalized to GAPDH amplification products. Transcript X1 (black bars) & transcript X2 (white bars), encoded by ephrin-B2 gene, were detected & represented (C). Data are shown as mean ± SEM. *P < 0.05 vs. N mice, one-way ANOVA followed by Bonferroni’s post-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84830_rabbit-polyclonal-ephrin-b2-antibody-31020241539743.jpg)
