Desmoglein-3 Antibody (5G11)

Images

 
Immunocytochemistry/ Immunofluorescence: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 in A-431 cells.
Immunohistochemistry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 on human skin cancer. NB100-1643 was used at a dilution of 1:200.
Flow Cytometry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
5G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Desmoglein-3 Antibody (5G11) Summary

Immunogen
Human Desmoglein 3 recombinant protein [UniProt# P32926]
Localization
Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome
Specificity
This antibody reacts with Desmoglein 3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DSG3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Dot Blot
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Use in Dot blot reported in scientific literature (PMID: 15588331).
Publications
Read Publications using
NB100-1643 in the following applications:

  • DB
    1 publication
  • 2 publications
  • IHC
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Desmoglein-3 Antibody (5G11)

  • Cadherin family member 6
  • CDHF6
  • CDHF6130-kD pemphigus vulgaris antigen
  • desmoglein 3 (pemphigus vulgaris antigen)
  • desmoglein 3
  • Desmoglein3
  • Desmoglein-3
  • DKFZp686P23184,130 kDa pemphigus vulgaris antigen
  • DSG3
  • pemphigus vulgaris antigen
  • PVA

Background

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmoglein-3 Antibody (NB100-1643)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: DB, ICC/IF, IHC.


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DB
(1)
ICC/IF
(2)
IHC
(2)
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Human
(4)
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Product General Protocols

View specific protocols for Desmoglein-3 Antibody (NB100-1643): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Desmoglein-3 Antibody (NB100-1643) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1643AF350
Alexa Fluor 405 NB100-1643AF405
Alexa Fluor 488 NB100-1643AF488
Alexa Fluor 532 NB100-1643AF532
Alexa Fluor 594 NB100-1643AF594
Alexa Fluor 647 NB100-1643AF647
Alexa Fluor 700 NB100-1643AF700
Alexa Fluor 750 NB100-1643AF750
Allophycocyanin NB100-1643APC
Allophycocyanin/Cy7 NB100-1643APCCY7
Biotin NB100-1643B
DyLight 350 NB100-1643UV
DyLight 405 NB100-1643V
DyLight 488 NB100-1643G
DyLight 550 NB100-1643R
DyLight 594 NB100-1643DL594
DyLight 650 NB100-1643C
DyLight 680 NB100-1643FR
DyLight 755 NB100-1643IR
FITC NB100-1643F
HRP NB100-1643H
Janelia Fluor 549 NB100-1643JF549
Janelia Fluor 646 NB100-1643JF646
PE NB100-1643PE
PE/Atto594 NB100-1643PEATT594
PE/Cy5.5 NB100-1643PECY55
PE/Cy7 NB100-1643PECY7

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Blogs on Desmoglein-3.

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Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

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Bioinformatics

Gene Symbol DSG3
Entrez
Uniprot