Desmoglein-3 Antibody (5G11) - BSA Free

Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 in A-431 cells.

Immunohistochemistry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Analysis of Desmoglein-3 on human skin cancer. NB100-1643 was used at a dilution of 1:200.

Flow Cytometry: Desmoglein-3 Antibody (5G11) [NB100-1643] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Desmoglein-3 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

• Reactivity: Hu
• Applications: WB, DB, Flow, ICC/IF, IHC, IP
• Clone: 5G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Desmoglein-3 Antibody (5G11) - BSA Free Summary

• Immunogen: Partial recombinant Human Desmoglein 3 protein expressed in baculovirus (amino acids 1-615)[UniProt# P32926]
• Localization: Cell membrane; Single-pass type I membrane protein. Cell junction > desmosome
• Specificity: This antibody reacts with Desmoglein-3. Does not cross-react with Desmoglein 1 or Desmoglein 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSG3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot reported in scientific literature (PMID 15588331)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Desmoglein-3 Antibody (5G11) - BSA Free

• Cadherin family member 6
• CDHF6
• CDHF6130-kD pemphigus vulgaris antigen
• desmoglein 3 (pemphigus vulgaris antigen)
• desmoglein 3
• Desmoglein3
• Desmoglein-3
• DKFZp686P23184,130 kDa pemphigus vulgaris antigen
• DSG3
• pemphigus vulgaris antigen
• PVA

Background

Desmoglein 3 (Dsg3) is a member of sub-family the desmoglein (DSGs) and it mainly serves as adhesion component of intercellular desmosome junctions. DSGs serve essential part of the core complex of desmosomes, and together with desmocollin (DSCs), they represents transmembrane proteins which mediate adhesion by transinteracting with cadherins of the opposing cell. Homophilic (e.g., DSG3-DSG3) as well as heterophilic (e.g., DSC3-DSG1) transinteraction have been reported for these proteins and within the desmosome, DSGs/DSCs are anchored to the intracellular plaque proteins plakoglobin, plakophilins, and desmoplakin, the latter of which mediates connection to the intermediate filament cytoskeleton. Desmoglein 3 is predominately expressed in stratified squamous epithelia including epidermis, tongue, tonsil, esophagus and carcinomas. Desmoglein 3 has been characterized as an auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease, wherein the binding of anti-desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmoglein-3 Antibody (NB100-1643)(6)

Publications using NB100-1643

• Griffin JR, Wriston CC, Peters MS, Lehman JS. Decreased expression of intercellular adhesion molecules in acantholytic squamous cell carcinoma compared with invasive well-differentiated squamous cell carcinoma of the skin Am J Clin Pathol 2013-04-01 [PMID: 23525614]
• Roden AC, Yi ES, Cassivi SD et al. Clinicopathological features of thymic carcinomas and the impact of histopathological agreement on prognostical studies Eur J Cardiothorac Surg 2012-10-19 [PMID: 23086701] (IF/IHC, Human)
• Lucchese A, Mittelman A, Lin MS, Kanduc D, Sinha AA. Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10. J Transl Med. 2(1):43. 2004-12-11 [PMID: 15588331] (Cytometric Bead Assay Standard, Human)
• Wu H, Stanley JR, Cotsarelis G. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol. 2003-01-01 [PMID: 12787134] (ICC/IF, IF/IHC, Human)
• Wahl JK 3rd. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics. 21(1):37-44. 2002-02-01 [PMID: 11991815]
• Wu H, Wang ZH, Yan A, Lyle S, Fakharzadeh S, Wahl JK, Wheelock MJ, Ishikawa H, Uitto J, Amagai M, Stanley JR. Protection against pemphigus foliaceus by desmoglein 3 in neonates. N Engl J Med;343(1):31-5. 2000-07-06 [PMID: 10882766] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: DSG3
• Entrez:
  • 1830(Human)
• Uniprot:
  • P32926()