STIM1 Antibody - BSA Free

Western Blot: STIM1 Antibody [NB110-60547] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stim-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Stim-1 Polyclonal Antibody (Catalog # NB110-60547) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stim-1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: STIM1 Antibody [NB110-60547] - Stromal interaction molecule 1 Antibody [NB110-60547] - Detection of STIM1 in HeLa whole cell extracts.

Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NB110-60547] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Po
• Applications: WB, Simple Western, ICC/IF, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

STIM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit STIM1 Antibody - BSA Free (NB110-60547) is a polyclonal antibody validated for use in WB, ICC/IF and Simple Western. Anti-STIM1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: STIM1 antibody was made to a synthetic peptide from the C-terminal region (within residues 600-685) of the human STIM1 protein. [UniProt# Q13586]
• Localization: Cell membrane, ER membrane. Translocates from ER to cell membrane in response to depletion of intracellular calcium.
• Predicted Species: Porcine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Knockdown Validated 1:1000
  • Simple Western 1:200
  • Western Blot 1:1000
• Application Notes: This STIM1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In Western Blot a band is seen at ~77 kDa, representing the STIM1 protein. Two other non-specific bands of lower MW can also be seen.
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  STIM1 Overexpression Lysate

• Control Peptide:

  STIM1 Peptide (NB110-60547PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: porcine (93%) and Xenopus (79%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STIM1 Antibody - BSA Free

• GOK
• GOKD11S4896E
• STIM1
• stromal interaction molecule 1

Background

Stromal interaction molecule 1 (STIM1 or GOK) is a type 1 transmembrane protein that mediates store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. STIM1 is Ca2+ sensor that conveys information about ER lumen's Ca2+ load to store-operated Ca2+ channels. It exists as a single transmembrane protein located in ER membrane with a luminal EF hand and a cytosolic domain, which activates Ca2+ release-activated Ca2+ channels. Upon Ca2+ depletion, STIM1 translocates from ER to plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1 resulting in a strong Ca2+ influx. STIM1 is one of several genes located in imprinted gene domain of 11p15.5, an important tumor-suppressor gene region and alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. STIM1 implicates in early hematopoiesis by mediating attachment to stromal cells and defective STIM1 have been linked to immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STIM1 Antibody (NB110-60547)(7)

Publications using NB110-60547

• Fuchs C, Stalnaker KJ, Dalgard CL et al. Plantar Skin Exhibits Altered Physiology, Constitutive Activation of Wound-Associated Phenotypes, and Inherently Delayed Healing. The Journal of investigative dermatology 2024-06-22 [PMID: 38237729]
• Angulo J, Fern�ndez A, Sevilleja-Ortiz A et al. Upregulation of Orai Channels Contributes to Aging-Related Vascular Alterations in Rat Coronary Arteries International Journal of Molecular Sciences 2023-08-29 [PMID: 37686206] (Western Blot, Rat)
• Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mech Ageing Dev 2021-10-26 [PMID: 34699858] (IF/IHC, Human, Rat)
  
  Details:
  Citation using the HRP version of this antibody.
• Bugajev V, Paulenda T, Utekal P et al. Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways Journal of lipid research 2021-09-21 [PMID: 34560079] (ICC/IF, Human)
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Nishi H, Arai H, Momiyama T. NCI-H295R, a Human Adrenal Cortex-Derived Cell Line, Expresses Purinergic Receptors Linked to Ca2+-Mobilization/Influx and Cortisol Secretion. PLoS One. 2013-08-08 [PMID: 23951072] (WB, Human)
• Meuchel LW, Stewart A, Smelter DF et al. Neurokinin-Neurotrophin Interactions in Airway Smooth Muscle. Am J Physiol Lung Cell Mol Physiol. 2011-04-22 [PMID: 21515660] (WB, Rat)

Bioinformatics

• Gene Symbol: STIM1