STIM1 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PoSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

STIM1 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to a C-terminal region (within residues 600-685) of the human Stromal interaction molecule 1 protein. [UniProt# Q13586]
Localization
Cell membrane, ER membrane. Translocates from ER to cell membrane in response to depletion of intracellular calcium.
Predicted Species
Porcine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STIM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Stromal interaction molecule 1 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB a band is seen at ~77 kDa, representing the STIM-1 protein. Two other non-specific bands of lower MW can also be seen.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, bovine and canine. Immunogen has 93% homology to porcine and 79% homology to Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for STIM1 Antibody [DyLight 650]

  • GOK
  • GOKD11S4896E
  • STIM1
  • stromal interaction molecule 1

Background

Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STIM1 Antibody (NB110-60547C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB110-60547B
DyLight 488 Labeled NB110-60547G
DyLight 550 Labeled NB110-60547R
HRP Labeled NB110-60547H

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Bioinformatics

Gene Symbol STIM1
Entrez