YAP1 Antibody (1A12)

Images

 
Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Western blot analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate.
Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining.
Flow Cytometry: YAP1 Antibody (1A12) [NBP2-22117] - Flow cytometric analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red).
Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Western blot analysis using YAP1 mAb against human YAP1 (AA: 250-447) recombinant protein. (Expected MW is 54.4 kDa)
Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining.
ELISA: YAP1 Antibody (1A12) [NBP2-22117] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
1A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

YAP1 Antibody (1A12) Summary

Immunogen
Purified recombinant fragment of human YAP1 expressed in E. coli. [UniProt# P46937]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
YAP1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This YAP1 (1A12) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for YAP1 Antibody (1A12)

  • 65 kDa Yes-associated protein
  • YAP
  • YAP1
  • YAP2
  • YAP65
  • YAP65yes-associated protein 2
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • YKI
  • Yorkie Homolog

Background

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Bioinformatics

Gene Symbol YAP1
Entrez
Uniprot