| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2379SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49672SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82553-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1831SS | |||
| Azide and BSA Free | NBP2-80961 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18946SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18951SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18950SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71225 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18949SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18953SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-18872SS | |||
![Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0014.jpg "Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license.")
![Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0011.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-2379 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2379-img0018.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and Western blot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0016.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NB100-2379] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of OCT4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-NB100-2379-img0017.jpg)
![Flow Cytometry: OCT4 Antibody - BSA Free [NB100-2379] - An intracellular stain was performed on U2-OS cells with OCT4 NB100-2379AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/OCT4-Antibody---BSA-Free-Flow-Cytometry-NB100-2379-img0022.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0013.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0019.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Neuro2a cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0020.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Ntera2 cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0021.jpg)
![Western Blot: OCT4 Antibody - BSA Free [NB100-2379] - Protein expression of NANOG a, OCT4 b & SOX2 c in bovine uterine tissues obtained from control cows & from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal & adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 & GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2379_rabbit-polyclonal-oct4-antibody-310202415394524.jpg)
![Western Blot: OCT4 Antibody - BSA Free [NB100-2379] - Protein expression of pluripotency markers in uterine cells isolated from control cows & from cows with adenomyosis. NANOG a OCT4 b & SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e & SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal & adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 & GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2379_rabbit-polyclonal-oct4-antibody-310202415384144.jpg)
![Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Western-Blot-NBP1-49672-img0004.jpg "Western Blot: NKX6.1 Antibody [NBP1-49672] - Analysis of Nkx6.1 in human skeletal muscle.")
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-49672] - Nkx6.1 antibody was tested at 1:250 in INS-1 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49672-img0006.jpg)
![Immunohistochemistry: NKX6.1 Antibody - BSA Free [NBP1-49672] - A) Representative immunofluorescence images of pancreatic sections with NKX6.1 and Insulin (Ins). B) Relationships among different dedifferentiated beta cell labeled by white boxes in (a). Scale bars: 20 um, Red: NKX6.1, Green: Ins, Blue: DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33711989/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX6.1-Antibody---BSA-Free-Immunohistochemistry-NBP1-49672-img0012.jpg)
![Immunohistochemistry-Frozen: NKX6.1 Antibody [NBP1-49672] - Analysis of NKX6.1 in mouse pancreas frozen tissue section using anti-NKX6.1 antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Frozen-NBP1-49672-img0009.jpg)
![Immunohistochemistry: NKX6.1 Antibody [NBP1-49672] - Staining of Nkx6.1 in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-NBP1-49672-img0003.jpg)
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Human fetal pancreas stained for Nkx6.1, green, and PDX1, grey. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-49672-img0007.jpg)
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Mouse pancreas tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-49672-img0010.jpg)
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-49672] - Paraffin embeded rat pancreatic tissue at 40x resolution stained for insulin (red) and NKX6.1 (NBP1-49672, green). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-49672-img0011.jpg)
![Simple Western: NKX6.1 Antibody [NBP1-49672] - Image shows a specific band for NKX6.1 in 0.5 mg/mL of BTC-6 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Simple-Western-NBP1-49672-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57305-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0017.jpg "Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57305-x), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Wnt-modulation influences the distribution of mono-hormonal and bi-hormonal S7 cells. IF analysis of NKX6.1+ (red), PDX1+ (green) cells and NKX6.1+/PDX1+ cells (yellow). Scale bar upper panel: 25 um, lower panel: 7.5 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fendo.2019.00293/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0018.jpg)
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0010.jpg)
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Immunocytochemistry-Immunofluorescence-NB100-1831-img0013.jpg "Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
![Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Flow-Cytometry-NB100-1831-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Immunocytochemistry-Immunofluorescence-NB100-1831-img0006.jpg)
![Simple Western: SSEA-1 Antibody (MC-480) [NB100-1831] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SSEA-1-Antibody-MC-480-Simple-Western-NB100-1831-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPi2-Antibody-HIC1-2B4-2B-Immunocytochemistry-Immunofluorescence-NBP1-18946-img0009.jpg "Immunocytochemistry/Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPi2-Antibody-HIC1-2B4-2B-Flow-Cytometry-NBP1-18946-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HPx1 Antibody (HIC0-3B3) [NBP1-18951] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPx1-Antibody-HIC0-3B3-Immunocytochemistry-Immunofluorescence-NBP1-18951-img0003.jpg "Immunocytochemistry/Immunofluorescence: HPx1 Antibody (HIC0-3B3) [NBP1-18951] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPx1 Antibody (HIC0-3B3) [NBP1-18951] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPx1-Antibody-HIC0-3B3-Flow-Cytometry-NBP1-18951-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPa2-Antibody-DHIC2-2B4-Immunocytochemistry-Immunofluorescence-NBP1-18950-img0003.jpg "Immunocytochemistry/Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPa2-Antibody-DHIC2-2B4-Flow-Cytometry-NBP1-18950-img0004.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0013.jpg "Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0016.jpg)
![Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Flow-Cytometry-NBP2-15052-img0009.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Western-Blot-NBP2-15052-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunocytochemistry-Immunofluorescence-NBP2-15052-img0008.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0001.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunohistochemistry-Paraffin-NBP2-15052-img0012.jpg)
![Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.](http://images.novusbio.com/fullsize/OCT4-Antibody-486-Immunoprecipitation-NBP2-15052-img0010.jpg)
![Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-910202420134071.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-91020242011495.jpg)
![Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-9102024202468.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-1610202419405748.jpg)
![Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ug human ESC whole cell lysate/extract10% SDS-PAGE (NBP2-15052) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15052_mouse-monoclonal-oct4-antibody-486-western-blot-2611202518301214.jpg)
![Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Western-Blot-NBP1-47923-img0007.jpg "Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4")
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY 41186.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunocytochemistry-Immunofluorescence-NBP1-47923-img0006.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human Ovary tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0004.jpg)
![Western Blot: OCT4 Antibody (OTI9B7) [NBP1-47923] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Western-Blot-NBP1-47923-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative IF IHC-stained sections of MDBMSCC. Expression of both SOX2 (red) and OCT4 (green), appearing as orange, was seen on cells within the tumor nests and the stroma, and the endothelium of the microvessels within the stroma. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunocytochemistry-Immunofluorescence-NBP1-47923-img0008.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human endometrium tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0002.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody (OTI9B7) [NBP1-47923] - Staining of paraffin-embedded Human lung tissue using anti-41186 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-OTI9B7-Immunohistochemistry-Paraffin-NBP1-47923-img0003.jpg)
![Immunohistochemistry: OCT4 Antibody (OTI9B7) [NBP1-47923] - Representative DAB IHC-stained sections of MDBMSCC demonstrating nuclear expression of EMA of cells within the tumor nests [(A), brown]. Expression of SOX2 was seen in cells within the tumor nests [(B), brown, thick arrows] & the stroma [(B), brown, arrowheads], & on the endothelium of the microvessels within the stroma [(B), brown, thin arrows]. Expression of SALL4 was limited to cells within the tumor nests [(C), brown, thick arrows] & the endothelium of the microvessels [(C), brown, thin arrows]. OCT4 was also expressed in cells within tumor nests [(D), brown, thick arrows] & the stroma [(D), brown, arrowheads], & the endothelium of the microvessels within the stroma [(D), brown, thin arrows]. Expression of pSTAT3 was detected on cells within the tumor nests [(E), brown, thick arrows] & the stroma [(E), brown, arrowheads], & the endothelium of the microvessels within the stroma [(E), brown, thin arrows]. NANOG was also seen in cells within the tumor nests [(F), brown, thick arrows] & the stroma [(F), brown, arrowheads], & the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. CD44 expression was seen as membranous staining of the tumor nest cells [(G), brown]. Original magnification: 400×. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47923_mouse-oct4-mab-oti9b7-310202416165645.jpg)
![Immunocytochemistry/Immunofluorescence: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPa1-Antibody-DHIC2-2C12-Immunocytochemistry-Immunofluorescence-NBP1-18949-img0003.jpg "Immunocytochemistry/Immunofluorescence: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis using the DyLight 550 conjugate of NBP1-18949. Staining of KB, KB-VCR. Fixed in 4% paraformaldehyde.](http://images.novusbio.com/fullsize/HPa1-Antibody-DHIC2-2C12-Flow-Cytometry-NBP1-18949-img0005.jpg)
![Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPa1-Antibody-DHIC2-2C12-Flow-Cytometry-NBP1-18949-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Immunocytochemistry-Immunofluorescence-NBP1-18953-img0005.jpg "Immunocytochemistry/Immunofluorescence: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Immunofluorescence on frozen section of human pancreas.")
![Flow Cytometry: HPd1 Antibody (DHIC2-4A10) [NBP1-18953] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPd1-Antibody-DHIC2-4A10-Flow-Cytometry-NBP1-18953-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HPi1 Antibody (HIC0-4F9) [NBP1-18872] - Frozen section of human pancreas.](http://images.novusbio.com/fullsize/HPi1-Antibody-HIC0-4F9-Immunocytochemistry-Immunofluorescence-NBP1-18872-img0007.jpg "Immunocytochemistry/Immunofluorescence: HPi1 Antibody (HIC0-4F9) [NBP1-18872] - Frozen section of human pancreas.")
![Flow Cytometry: HPi1 Antibody (HIC0-4F9) [NBP1-18872] - Analysis of enzyme dispersed human pancreas cells.](http://images.novusbio.com/fullsize/HPi1-Antibody-HIC0-4F9-Flow-Cytometry-NBP1-18872-img0006.jpg)