HPa1 Antibody (DHIC2-2C12)

Images

 
Immunocytochemistry/ Immunofluorescence: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Immunofluorescence on frozen section of human pancreas.
Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis using the DyLight 550 conjugate of NBP1-18949. Staining of KB, KB-VCR. Fixed in 4% paraformaldehyde.
Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis of enzyme dispersed human pancreas cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr
Clone
DHIC2-2C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

HPa1 Antibody (DHIC2-2C12) Summary

Immunogen
Human pancreatic enriched alpha cells
Localization
Cell surface
Marker
Alpha Cell Marker
Specificity
Specific for alpha endocrine cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
Application Notes
This HPa1 (DHIC2-2C12) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence, Flow cytometry and Western blot.
Publications
Read Publications using
NBP1-18949 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Tissue culture supernatant

Notes

The estimated concentration for this antibody is 1.0 mg/ml.

Alternate Names for HPa1 Antibody (DHIC2-2C12)

  • human pancreatic alpha cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPa1 Antibody (NBP1-18949)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, IHC-Fr.


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FLOW
(1)
IHC-Fr
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HPa1 Antibody (NBP1-18949) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-18949AF405
Alexa Fluor 488 Labeled NBP1-18949AF488
Alexa Fluor 647 Labeled NBP1-18949AF647
Alexa Fluor 700 Labeled NBP1-18949AF700
Allophycocyanin Labeled NBP1-18949APC
Biotin Labeled NBP1-18949B
DyLight 350 Labeled NBP1-18949UV
DyLight 405 Labeled NBP1-18949V
DyLight 488 Labeled NBP1-18949G
DyLight 550 Labeled NBP1-18949R
DyLight 650 Labeled NBP1-18949C
DyLight 680 Labeled NBP1-18949FR
DyLight 755 Labeled NBP1-18949IR
FITC Labeled NBP1-18949F
HRP Labeled NBP1-18949H
PE Labeled NBP1-18949PE
PerCP Labeled NBP1-18949PCP

Additional HPa1 Products

HPa1 NBP1-18949

Research Areas for HPa1 Antibody (NBP1-18949)

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Blogs on HPa1

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