HPi2 Antibody (HIC1-2B4.2B) [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC
• Clone: HIC1-2B4.2B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

HPi2 Antibody (HIC1-2B4.2B) [Alexa Fluor® 488] Summary

• Description: This product is provided as conjugated tissue culture supernatant. Due to the crude preparation of the antibody material, excess dye may remain in the final product. We highly recommend taking necessary precautions, such as adding additional wash steps and running proper controls.
• Immunogen: Human pancreatic enriched islet cells containing low levels of exocrine and ductal cells.
• Localization: Cell surface
• Marker: Endocrine pan-islet cell Marker
• Specificity: Specific for multiple endocrine cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26515645).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HPi2 Antibody (HIC1-2B4.2B) [Alexa Fluor® 488]

• human pancreatic endocrine islet cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPi2 Antibody (NBP1-18946AF488)(1)

Publication using NBP1-18946AF488

• Alvarez-Do JR, Donaghey J,Rasouli N et al. Circadian Entrainment Triggers Maturation of Human InVitro Islets Cell Stem Cell. [PMID: 31839570] (Human)