SSEA-1 Antibody (MC-480)

Images

 
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight ...read more
Simple Western: SSEA-1 Antibody (MC-480) [NB100-1831] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
MC-480
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SSEA-1 Antibody (MC-480) Summary

Immunogen
F9 tetracarcinoma stem cells (X-irradiated).
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:10
  • Flow Cytometry 2.5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NB100-1831 in the following applications:

Reactivity Notes

This antibody recognizes SSEA1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for SSEA-1 Antibody (MC-480)

  • alpha-(1,3)-fucosyltransferase
  • CD15
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • ELAM-1 ligand fucosyltransferase
  • ELFT
  • FCT3AELAM ligand fucosyltransferase
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fucosyltransferase 4
  • Fucosyltransferase IV
  • FUC-TIV
  • fucT-IV
  • FUTIV
  • Galactoside 3-L-fucosyltransferase
  • Lewis X
  • LeX
  • SSEA1
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1831
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for SSEA-1 Antibody (NB100-1831)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(2)
ICC/IF
(1)
WB
(1)
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Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-1831 Applications Species
Zhang M, Wang C, Jiang H et al. Derivation of novel naive-like porcine embryonic stem cells by a reprogramming factor-assisted strategy FASEB J. May 24 2019 [PMID: 31125263]
Meng S, Su Z, Liu Z et al. Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2. Neuroscience. 2015 Aug 20 [PMID: 26299339] (WB, Mouse)

Details:
SSEA1 antibody was used at 2ug/ml concentration for WB assay on lysates of neuron stem-like cells that were treated with Notch inhibitor DAPT, Notch2 siRNA or Rac1 siRNA, or a combination of Rac1 siRNA with NICD2 (Fig. 7C).
WB Mouse
Martin, M et al. Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells;22(5):659-68. 2004 [PMID: 15342930] (FLOW, Human) FLOW Human
Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, Shinohara T. Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod;69(2):612-6. 2003 Aug. [PMID: 12700182] (FLOW, Mouse) FLOW Mouse
Henderson, JK et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells;20(4):329-37. 2002 [PMID: 12110702]
Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol;18(4):399-404. 2000 Apr. [PMID: 10748519] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FUT4
Entrez