SSEA-1 Antibody (MC-480)

Images

 
Western Blot: SSEA-1 Antibody (MC-480) [NB100-1831] - Western blot analysis of Ntera2 cell lysate (A), HUVEC cell lysate (B), Jurkat cell lysate (C), human stomach tissue (D), human brain tissue (E), MEF cell lysate ...read more
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - Staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight ...read more
Simple Western: SSEA-1 Antibody (MC-480) [NB100-1831] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
MC-480
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SSEA-1 Antibody (MC-480) Summary

Immunogen
F9 tetracarcinoma stem cells (X-irradiated).
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
FUT4
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:10
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NB100-1831 in the following applications:

Reactivity Notes

This antibody recognizes SSEA1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for SSEA-1 Antibody (MC-480)

  • alpha-(1,3)-fucosyltransferase
  • CD15
  • EC 2.4.1
  • EC 2.4.1.-
  • EC 2.4.1.65
  • ELAM-1 ligand fucosyltransferase
  • ELFT
  • FCT3AELAM ligand fucosyltransferase
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fucosyltransferase 4
  • Fucosyltransferase IV
  • FUC-TIV
  • fucT-IV
  • FUTIV
  • Galactoside 3-L-fucosyltransferase
  • Lewis X
  • LeX
  • SSEA1
  • SSEA-1
  • stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SSEA-1 Antibody (NB100-1831)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(2)
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species

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Product General Protocols

View specific protocols for SSEA-1 Antibody (NB100-1831): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SSEA-1 Antibody (NB100-1831) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1831AF405
Alexa Fluor 488 Labeled NB100-1831AF488
Alexa Fluor 647 Labeled NB100-1831AF647
Alexa Fluor 700 Labeled NB100-1831AF700
Allophycocyanin Labeled NB100-1831APC
Biotin Labeled NB100-1831B
DyLight 350 Labeled NB100-1831UV
DyLight 405 Labeled NB100-1831V
DyLight 488 Labeled NB100-1831G
DyLight 550 Labeled NB100-1831R
DyLight 650 Labeled NB100-1831C
DyLight 680 Labeled NB100-1831FR
DyLight 755 Labeled NB100-1831IR
FITC Labeled NB100-1831F
HRP Labeled NB100-1831H
PE Labeled NB100-1831PE
PerCP Labeled NB100-1831PCP

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Bioinformatics

Gene Symbol FUT4
Entrez