Western Blot: SSEA-1 Antibody (MC-480) [NB100-1831] - Western blot analysis of Ntera2 cell lysate (A), HUVEC cell lysate (B), Jurkat cell lysate (C), human stomach tissue (D), human brain tissue (E), MEF cell lysate ...read more
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - Staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) [NB100-1831] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight ...read more
Simple Western: SSEA-1 Antibody (MC-480) [NB100-1831] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Western Blot 2 ug/ml
Simple Western 1:10
Flow Cytometry 1:50-1:200
This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NB100-1831 in the following applications:
This antibody recognizes SSEA1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530).
SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.