HPa2 Antibody (DHIC2-2B4) - BSA Free

Immunocytochemistry/Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.

Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Analysis of enzyme dispersed human pancreas cells.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: DHIC2-2B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HPa2 Antibody (DHIC2-2B4) - BSA Free Summary

• Description: Novus Biologicals Mouse HPa2 Antibody (DHIC2-2B4) - BSA Free (NBP1-18950) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-HPa2 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human pancreatic enriched alpha cells
• Localization: Cell surface
• Marker: Alpha Cell Marker
• Specificity: Specific for alpha endocrine cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Western Blot 1:100-1:2000
• Application Notes: This HPa2 (DHIC2-2B4) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence, Flow cytometry and Western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for HPa2 Antibody (DHIC2-2B4) - BSA Free

• human pancreatic alpha cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPa2 Antibody (NBP1-18950)(7)

Publications using NBP1-18950

• Acosta-Montalvo A, Saponaro C, Kerr-Conte J et al. Proglucagon-Derived Peptides Expression and Secretion in Rat Insulinoma INS-1 Cells Front Cell Dev Biol 2020-11-10 [PMID: 33240888] (ICC/IF)
• Arda H, Tsai J, Rosli Y et al A Chromatin Basis for Cell Lineage and Disease Risk in the Human Pancreas Cell Syst 2018-08-27 [PMID: 30145115] (FLOW, Human)
  
  Details:
  Citation using the DyLight 488 version of this antibody.
• Arda HE, Li L, Tsai J, et al. Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human beta Cell Function. Cell Press. 2016-05-10 [PMID: 27133132] (FLOW, Human)
• Caroline Bonner, Julie Kerr-Conte, Valery Gmyr et al. Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion. Nature Medicine 2015-04-20 [PMID: 25894829] (FLOW, Human)
• Nostro MC, Sarangi F, Ogawa S et al. Pancreatic differentiation 2008-01-01 [PMID: 23658992]
• Dorrell C, Schug J, Lin C et al. Transcriptomes of the major human pancreatic cell types. Diabetologia. 2011-01-01 [PMID: 21882062]
• Dorrell, et al. Isolation of major pancreatic cell types long-term culture-initiating cells using novel human surface markers. Stem Cell Rsch 1(3):183-194. 2008-01-01 [PMID: 19383399] (FLOW, IHC-Fr, Human)

FAQs for HPa2 Antibody (NBP1-18950). (Showing 1 - 2 of 2 FAQs).

1. I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?
   • I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.
2. Does HPa2 cross react with other species? Specifically mouse or monkey?
   • NBP1-18950 hasn't been tested in mouse or monkey but is predicted to cross react with both. It is not guaranteed for use in these species, but if you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program. Under this program by completing an online review with image, detailing your positive or negative results, you receive a discount voucher for 100% of the purchase price of the reviewed product.