HPa2 Antibody (DHIC2-2B4)

Images

 
Immunocytochemistry/ Immunofluorescence: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Immunofluorescence on frozen section of human pancreas.
Flow Cytometry: HPa2 Antibody (DHIC2-2B4) [NBP1-18950] - Analysis of enzyme dispersed human pancreas cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr
Clone
DHIC2-2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

HPa2 Antibody (DHIC2-2B4) Summary

Immunogen
Human pancreatic enriched alpha cells
Localization
Cell surface
Marker
Alpha Cell Marker
Specificity
Specific for alpha endocrine cell types.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
Application Notes
This HPa2 (DHIC2-2B4) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence, Flow cytometry and Western blot.
Publications
Read Publications using
NBP1-18950 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Tissue culture supernatant

Notes

The estimated concentration for this antibody is 1.0 mg/ml.

Alternate Names for HPa2 Antibody (DHIC2-2B4)

  • human pancreatic alpha cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPa2 Antibody (NBP1-18950)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HPa2 Antibody (NBP1-18950). (Showing 1 - 2 of 2 FAQs).

  1. I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?
    • I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.
  2. Does HPa2 cross react with other species? Specifically mouse or monkey?
    • NBP1-18950 hasn't been tested in mouse or monkey but is predicted to cross react with both. It is not guaranteed for use in these species, but if you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program. Under this program by completing an online review with image, detailing your positive or negative results, you receive a discount voucher for 100% of the purchase price of the reviewed product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-18950AF405
Alexa Fluor 488 Labeled NBP1-18950AF488
Alexa Fluor 647 Labeled NBP1-18950AF647
Alexa Fluor 700 Labeled NBP1-18950AF700
Allophycocyanin Labeled NBP1-18950APC
Biotin Labeled NBP1-18950B
DyLight 350 Labeled NBP1-18950UV
DyLight 405 Labeled NBP1-18950V
DyLight 488 Labeled NBP1-18950G
DyLight 550 Labeled NBP1-18950R
DyLight 650 Labeled NBP1-18950C
DyLight 680 Labeled NBP1-18950FR
DyLight 755 Labeled NBP1-18950IR
FITC Labeled NBP1-18950F
HRP Labeled NBP1-18950H
PE Labeled NBP1-18950PE
PerCP Labeled NBP1-18950PCP

Additional HPa2 Products

HPa2 NBP1-18950

Research Areas for HPa2 Antibody (NBP1-18950)

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