HPi3 Antibody (HIC1-7H10) - BSA Free

Immunocytochemistry/Immunofluorescence: HPi3 Antibody (HIC1-7H10) [NBP1-18947] - Immunofluorescence on frozen section of human pancreas.

Flow Cytometry: HPi3 Antibody (HIC1-7H10) [NBP1-18947] - Analysis of enzyme dispersed human pancreas cells.

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC
• Clone: HIC1-7H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HPi3 Antibody (HIC1-7H10) - BSA Free Summary

• Description: Novus Biologicals Mouse HPi3 Antibody (HIC1-7H10) - BSA Free (NBP1-18947) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. Anti-HPi3 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human pancreatic enriched islet cells containing low levels of exocrine and ductal cells.
• Localization: Cell surface
• Marker: Endocrine pan-islet cell Marker
• Specificity: Specific for multiple endocrine cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
• Application Notes: This HPi3 (HIC1-7H10) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence and Flow cytometry.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26515645).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for HPi3 Antibody (HIC1-7H10) - BSA Free

• human pancreatic endocrine islet cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPi3 Antibody (NBP1-18947)(4)

Publications using NBP1-18947

• Korytnikov R, Nostro MC. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2015-10-27 [PMID: 26515645]
• Nostro MC, Sarangi F, Ogawa S et al. Pancreatic differentiation 2008-01-01 [PMID: 23658992]
• Nostro MC, Sarangi F, Ogawa S, Holtzinger A, Corneo B, Li X, Micallef SJ, Park IH, Basford C, Wheeler MB, Daley GQ, Elefanty AG, Stanley EG, Keller G. Stage-specific signaling through TGFbeta family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells. Development;138(5):861-71. 2011-03-01 [PMID: 21270052] (FLOW, Human)
• Dorrell, et al. Isolation of major pancreatic cell types long-term culture-initiating cells using novel human surface markers. Stem Cell Rsch 1(3):183-194. 2008-01-01 [PMID: 19383399] (IHC-Fr, FLOW, Human)