HPi2 Antibody (HIC1-2B4.2B)

Images

 
Immunocytochemistry/ Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.
Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Analysis of enzyme dispersed human pancreas cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
HIC1-2B4.2B
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HPi2 Antibody (HIC1-2B4.2B) Summary

Immunogen
Human pancreatic enriched islet cells containing low levels of exocrine and ductal cells.
Localization
Cell surface
Marker
Endocrine pan-islet cell Marker
Specificity
Specific for multiple endocrine cell types.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
Application Notes
This HPi2 (HIC1-2B4.2B) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence and Flow cytometry.
Publications
Read Publications using
NBP1-18946 in the following applications:

  • 2 publications
  • 5 publications
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26515645).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for HPi2 Antibody (HIC1-2B4.2B)

  • human pancreatic endocrine islet cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPi2 Antibody (NBP1-18946)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, Flow, IHC-Fr, IP, WB.


Filter By Application
FLOW
(2)
Flow
(5)
IHC-Fr
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-18946 Applications Species
Marquez-Curtis LA, Dai XQ, Hang Y et al. Cryopreservation and post-thaw characterization of dissociated human islet cells PloS one Jan 26 2022 [PMID: 35081145] (Flow, Human) Flow Human
Ghazvini Zadeh EH, Huang Z, Xia J et al. ZIGIR, a Granule-Specific Zn2+ Indicator, Reveals Human Islet alpha Cell Heterogeneity Cell Rep Jul 14 2020 [PMID: 32668245] (Flow, Human) Flow Human
Camunas-Soler, J, Dai, X Q Et al. Patch-Seq Links Single-Cell Transcriptomes to Human Islet Dysfunction in Diabetes. Cell Metab May 5 2020 [PMID: 32302527] (IP, WB, Mouse) IP, WB Mouse
Alvarez-Do JR, Donaghey J,Rasouli N et al. Circadian Entrainment Triggers Maturation of Human InVitro Islets Cell Stem Cell. [PMID: 31839570] (Flow, Human) Flow Human
Arda H, Tsai J, Rosli Y et al A Chromatin Basis for Cell Lineage and Disease Risk in the Human Pancreas Cell Syst Aug 27 2018 [PMID: 30145115] (Flow, Human)

Details:
Citation using the DyLight 650 version of this antibody.
Flow Human
Jakel C, Bergmann F, Toth R et al. Genome-wide genetic and epigenetic analyses of pancreatic acinar cell carcinomas reveal aberrations in genome stability Nat Commun. 2017 Nov 06 [PMID: 29109526] (Human) Human
Enge M, Arda HE, Mignardi M et al. Single-Cell Analysis of Human Pancreas Reveals Transcriptional Signatures of Aging and Somatic Mutation Patterns. Autophagy. 2017 May 4 [PMID: 28965763] (Flow, Human)

Details:
Citation used the DyLight 650 format of this antibody.
Flow Human
Arda HE, Li L, Tsai J, et al. Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human beta Cell Function. Cell Press. 2016 May 10 [PMID: 27133132] (FLOW, Human) FLOW Human
Korytnikov R, Nostro MC. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2015 Oct 27 [PMID: 26515645]
Dorrell C, Schug J, Lin C et al. Transcriptomes of the major human pancreatic cell types. Diabetologia. 2011 [PMID: 21882062]
Show All 11 Publications.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for HPi2 Antibody (NBP1-18946). (Showing 1 - 1 of 1 FAQs).

  1. I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?
    • I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.

Secondary Antibodies

 

Isotype Controls

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Array NBP1-18946

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