| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-232SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-229SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1612-0.05ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48336SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48309SS | |||
| Azide and BSA Free | NBP2-80868 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-231SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2379SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05767SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33130 | |||
| IHC-Prediluted | NBP2-45339 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-232-img0013.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Chromosome spread for sycp3. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-232] - IHC analysis of a formalin fixed and paraffin embedded tissue section of Mouse testis using SCP3 antibody at 1:1000 dilution. The signal was detected using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a specific signal of SCP3 in the spermatogonial cells and the spermatocytes. The signal was strongest in the nuclei of spermatogonial cells.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NB300-232-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) and Hoechst 33342 (blue). All experiments were repeated at least three times independently. (* P600 spermatocytes in each stage. b, c The immunostaining of SYCP3 in the testicular sections (b) & surface-spread chromatin preparations of Uhrf1 deletion & control mice (c); d the percentage of spermatocytes with abnormal SYCP3 location. e Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) & SYCP1 (red). f The percentage of spermatocytes with abnormal SYCP1 location. Lep leptotene, Zyg zygotene, Pac pachytene, Dip diplotene. Data are presented as mean ± SEM of three mice. ***p ≤ 0.001. Scale bar, 25 μm in b, 5 μm in c, e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-31020241621832.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - Dynamic expression of germ cell markers in human fetal ovaries. (A) Histological sections of human ovaries at W10.5, W14, W17 & W21.5, immunostained for the early germ cell marker (nuclear) POU5F1 (red) & late germ cell marker (cytoplasmic) DDX4 (green). In zone 1, most germ cells are POU5F1+DDX4−/low; in zone 2 & 3, most germ cells are DDX4+. Several germ cells in zone 3 have developed into primordial follicles. Inserts are magnifications of the dotted boxes. (B) Histological sections of human ovaries at W10.5, W14, W17 & W21.5, immunostained for the meiotic markers H2AFX (red) & SYCP3 (green). Inserts are magnifications of the dotted boxes. Note the presence of autofluorescent red blood cells. Scale bars are 200 µm & in the magnified inserts 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26834021), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-31020241620376.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - CYP51 regulates the expression of the meiosis-specific cohesin subunits REC8 & STAG3. (A,B) RS21745 treatment decreased REC8 & STAG3 expression. Ovaries at 14.5 dpc were cultured with 10 μM RS21745 for 3 days in vitro. (A) Expression of the cohesin subunits following RS21745 treatment by qRT-PCR. All qRT-PCR values were normalized to beta -actin & were expressed as a relative ratio to the control; the means±s.e.m. of 3 values are shown. (B) Expression of REC8 & STAG3 following RS21745 treatment by western blotting GAPDH was used as internal reference. (C–F) Inhibition of CYP51 disturbed the distribution of both REC8 & STAG3 on the chromosomes. (C,E) In the control groups, REC8 & STAG3 expression was observed at high levels in zygotene oocytes. In the CYP51 inhibition groups, the cohesin signals were absent in zygotene cells. (D,F) The percentage of REC8-positive or STAG3-positive oocytes at the zygotene stage was recorded. Unidentified germ cells were not included in the analyses. Scale bars: 10 μm. The data are presented as the means±s.e.m. of 3–9 ovaries per group. Asterisk (*) denotes a statistically significant difference between the control & the treatment groups. ***P<0.001 (t-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30420384), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-310202416212332.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - Induction of meiosis is compromised in the cortical germ cells of D17 embryos subject to oestrogen level alterations. (A) D17 (HH43) left gonad sections immunostained for gamma H2AX (green) & P63 (red); nuclei are counterstained with DAPI (blue). (B) Sections from the D17 (HH43) left gonad shown in A immunostained for gamma H2AX (green) & SYCP3 (red). ZW-WT, ZW wild type; ZW-Fa, ZW treated with fadrozole from D7-7.5 (HH31); ZW-E2, ZW treated with beta -oestradiol at D7-7.5; ZZ-E2, ZZ treated with beta -oestradiol at D7-7.5; ZW-Fa(sr), ZW, partially sex-reversed gonad, treated with fadrozole at D4; ZZ-E2(sr), ZZ, partially sex-reversed gonad, treated with beta -oestradiol at D4. All gonadal models have a cortical domain containing germ cells. In ZW-Fa ovary & ZW-E2 ovary, most cortical germ cells express SYCP3 like the ZW-WT control. In ZW-Fa(sr) ovotestis, very few germ cells express gamma H2AX & SYCP3 (orange dotted circled areas). In ZZ-E2(sr) ovotestis & ZZ-E2 testis overlain by a cortex, some cortical germ cells express gamma H2AX but none expresses SYCP3. White dotted line highlights the cortical domain borders. See Fig. S4 for the medullary structure of the ZZ-E2, ZW-Fa(sr) & ZZ-E2(sr) models. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-310202416212348.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - Exposure to DEHP impairs meiotic progression of oocytes from pachytene to diplotene. (a) Immunolabeling of the oocyte chromosomes with anti-SYCP3 antibody (red) & Hoechst 33342 (blue). (b) Effect of DEHP on meiotic progression of oocytes throughout prophase I stages; percentage of each group is presented as mean±SD. Control: 36.27±0.80% pachytene & 54.99±0.66% diplotene; 10 μM & 100 μM DEHP 57.79±4.22% & 56.62±6.62% pachytene & 39.79±4.22% & 36.62±6.62% diplotene, respectively. (c) Representative WB showing the effect of DEHP on the expression of germ cell (DAZL) & meiotic (STRA8 & SCP3) specific proteins. (d) Effect of DEHP on the levels of mRNA in the ovarian tissues of germ cell (Mvh & Dazl), meiotic (Stra8, Rec8, Scp1 & Scp3). All experiments were repeated at least three times independently. (* P<0.05; ** P<0.01) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-310202416212391.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.(A) 3D-preserved swab preparations showing wildtype (A) & knockout (A′) spermatocytes simultaneously labelled with anti-TRF1 & SUN1 antibodies. As in the wildtype, in lamin C2−/− spermatocytes virtual all telomeres appear to be attached to the NE as indicated by co-localisation of TRF1 & SUN1 signals. Scale bars 5 µm. (B) Quantifications of co-localised & non-co-localised TRF1/SUN1 signals (see A) revealed that ratios of co-localised to non-co-localised spots comparing wildtype & knockout spermatocytes show no significant difference (wildtype n = 33; lamin C2−/− n = 45; Pearson's Chi2 test p-value: 0.799). (C,D) Chromosome spread preparations of pachytene-like lamin C2−/− spermatocytes showing that all telomeres are associated with SUN1. In (C) TeloFISH & in (D) anti-SUN1 staining in co-localisation with SYCP3. Scale bars 10 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-310202416212363.jpg)
![Immunohistochemistry: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - FANCM expression in human fetal ovaries.(A) Relative FANCM mRNA abundance was measured by RT-qPCR in human fetal ovaries from 5 to 32 weeks post-fertilization (wpf). (B) Germ cells (D2-40+) & somatic cells (D2-40-) were sorted from three ovaries ranging from 8 to 12 wpf & FANCM expression was measured. ACTB was used to normalize FANCM expression in all samples. Dots represent different ovaries & the mean is indicated by the line. (C) Immunohistochemistry of FANCM in human fetal & adult ovaries. Fetal ovaries at 8 & 22 wpf & adult ovaries were studied. FANCM positive cells appear in yellow/brown color (monoclonal FANCM CV5.1 antibody, Novus Biologicals, Abingdon, UK). Ovarian sections were counterstained with hematoxylin (blue staining). Oo, oogonia; Pa, oocyte at the pachytene stage of meiosis I, D, oocyte at the diplotene stage of meiosis I; Pr, oocyte in primordial follicle. (D) Co-staining in 22 wpf ovaries, for FANCM (purple) & DDX4 (brown) confirmed the germ cell identity of FANCM-positive cells (left). Successive staining for FANCM & SYCP3 in the same section (panels a & b). Negative control performed with non-immune mouse IgG (right). Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29231814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-3102024162184.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody - BSA Free [NB300-232] - Loss of lamin C2 has no effect on meiotic telomere attachment.(A) 3D-preserved swab preparations showing wildtype (A) & knockout (A′) spermatocytes simultaneously labelled with anti-TRF1 & SUN1 antibodies. As in the wildtype, in lamin C2−/− spermatocytes virtual all telomeres appear to be attached to the NE as indicated by co-localisation of TRF1 & SUN1 signals. Scale bars 5 µm. (B) Quantifications of co-localised & non-co-localised TRF1/SUN1 signals (see A) revealed that ratios of co-localised to non-co-localised spots comparing wildtype & knockout spermatocytes show no significant difference (wildtype n = 33; lamin C2−/− n = 45; Pearson's Chi2 test p-value: 0.799). (C,D) Chromosome spread preparations of pachytene-like lamin C2−/− spermatocytes showing that all telomeres are associated with SUN1. In (C) TeloFISH & in (D) anti-SUN1 staining in co-localisation with SYCP3. Scale bars 10 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-232_rabbit-polyclonal-scp3-sycp3-antibody-310202416212314.jpg)
![Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SCP1-Antibody-Simple-Western-NB300-229-img0010.jpg "Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)](http://images.novusbio.com/fullsize/SCP1-Antibody-Western-Blot-NB300-229-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) and SCP1 (red). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0017.jpg)
![Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunohistochemistry-Paraffin-NB300-229-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0016.jpg)
![Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] - Meiotic spreads.Meiotic chromosome spreads from wild type & Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) & SYCP1 (green), which are axial element & central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) & abnormal SYCP3 structures (arrows). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159800), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-229_rabbit-polyclonal-scp1-antibody-310202415345326.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic progression & chromosome paring in XX, XO & XY oocytes.(A) Meiotic progression in XX, XO & XY oocytes. The graphs show the percentages of the meiotic stage at the day of culture indicated. L, leptotene; Z, zygotene; P, pachytene; D, diplotene. (B) Pairing of homologous chromosomes in XX, XO & XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) & SYCP1 (red), & FISH analysis of the X chromosome (green) & Y chromosome (purple). The dashed squares in the merged images are shown at high magnification (right). The numbers of samples showing the phenotype are shown with the total number tested (left). Arrowheads indicate asynapsed bivalents at the end of the chromosomes. Scale bars, 10 μm. (C) Pairing rates of autosomes & sex chromosomes. Each value was calculated from three independent experiments (see also Materials & Methods). P values were calculated by Tukey’s HSD test. ***P600 spermatocytes in each stage. b, c The immunostaining of SYCP3 in the testicular sections (b) & surface-spread chromatin preparations of Uhrf1 deletion & control mice (c); d the percentage of spermatocytes with abnormal SYCP3 location. e Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) & SYCP1 (red). f The percentage of spermatocytes with abnormal SYCP1 location. Lep leptotene, Zyg zygotene, Pac pachytene, Dip diplotene. Data are presented as mean ± SEM of three mice. ***p ≤ 0.001. Scale bar, 25 μm in b, 5 μm in c, e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-229_rabbit-polyclonal-scp1-antibody-310202416212369.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0006.jpg "Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using GFAP, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody [NB100-1612] - Beta-III Tubulin in rat primary motor neurons. Ab dilution 1:1000 in PBST (0.1% Triton X-100) + 10% GS O/N at 4C. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1612-img0011.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) and S100beta as astrocytic protein marker proteins in the hippocampal area. Western blot images using S100beta, tubulin beta-3, and beta-actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100beta/beta-tubulin ratio by beta-actin levels relative to young adult wild-type C57BL/6J. White and black bars indicate the wild-type C57BL/6J and Tet-mev-1 mice, respectively. Data are expressed as mean +/- SD; *P 12 in each group. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12523), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-Western-Blot-NB100-1612-img0007.jpg)
![Western Blot: beta-III Tubulin Antibody [NB100-1612] - The levels of glial fibrillary acidic protein (GFAP) & S100 beta as astrocytic protein marker proteins in the hippocampal area. (A) Western blot images using GFAP, tubulin beta‐3, & beta ‐actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized GFAP/ beta ‐tubulin ratio by beta ‐actin levels relative to young adult wild‐type C57BL/6J. White & black bars indicate the wild‐type C57BL/6J & Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P 12 in each group. (B) Western blot images using S100 beta , tubulin beta‐3, & beta ‐actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100 beta / beta ‐tubulin ratio by beta ‐actin levels relative to young adult wild‐type C57BL/6J. White & black bars indicate the wild‐type C57BL/6J & Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P 12 in each group. (C) Micrographs of immunohistochemical analysis on paraffined hippocampal tissue sections using GFAP & S100 beta antibody. Brown cells indicate GFAP‐stained astrocytes. Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27623715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1612_chicken-polyclonal-beta-iii-tubulin-antibody-310202415533839.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured by western blot assay. c LDHA protein levels were quantified by the ratio to B-actin in the hippocampus. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA in the cortex by the ratio to B-actin. Values are expressed as means +/- SEMs (n = 5 for both groups). * p < 0.05. Image collected and cropped by Citeab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0020.jpg "Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured by western blot assay. c LDHA protein levels were quantified by the ratio to B-actin in the hippocampus. f Cropped images of LDHA and LDHB in the cortex. g Quantified LDHA in the cortex by the ratio to B-actin. Values are expressed as means +/- SEMs (n = 5 for both groups). * p < 0.05. Image collected and cropped by Citeab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48336-img0009.jpg)
![Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunohistochemistry-NBP1-48336-img0013.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - An intracellular stain was performed on HeLa cells with Lactate Dehydrogenase A/LDHA Antibody NBP1-48336AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Flow-Cytometry-NBP1-48336-img0021.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0011.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0012.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0014.jpg)
![Western Blot: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Western-Blot-NBP1-48336-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - The effect of the co-culturing of cancer cells and fibroblasts on the expression of Hk2 and Ldha. In the co-cultures, the expression of Ldha was reduced in cancer cells, but was elevated in fibroblasts. Bar = 50 um. The ratio of the fluorescence of Hk2 and Ldha to beta-actin in the MLF monoculture was assumed to be 1. All the experiments were performed in triplicate and obtained similar results, and representative data from one experiment are shown in the figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31947613/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lactate Dehydrogenase A-LDHA Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-48336-img0022.jpg)
![Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Immunohistochemistry-NBP1-48336-img0010.jpg)
![Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336]](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Flow-Cytometry-NBP1-48336-img0017.jpg)
![Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-A-LDHA-Antibody---BSA-Free-Simple-Western-NBP1-48336-img0016.jpg)
![Simple Western: Lactate Dehydrogenase A/LDHA Antibody - BSA Free [NBP1-48336] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-48336_rabbit-polyclonal-lactate-dehydrogenase-a-ldha-antibody-310202416171412.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0025.jpg "Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.")
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0029.jpg)
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0005.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0026.jpg)
![Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Intracellular-NBP1-48309-img0010.jpg)
![Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Simple-Western-NBP1-48309-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0032.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0027.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0031.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0021.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-Paraffin-NBP1-48309-img0028.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0016.jpg)
![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg "Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.")
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin and parvalbumin antibodies shown in layer 2/3 at 63x magnification (scale = 10 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2013.00156/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in C6 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in Hela Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0007.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human cerebellum tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0003.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0004.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Parvalbumin Antibody - BSA Free [NB120-11427] - Stargazin expression is restricted to PV+ interneurons in WT mouse somatosensory cortex (upper row). Somatodendritic stargazin immunoreactivity is lost in PV+ interneurons in stargazer mouse (lower row). Co-labeling of stargazin & parvalbumin antibodies shown in layer 2/3 at 63× magnification (scale = 10 μm). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24065886), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202415284515.jpg)
![Immunohistochemistry: Parvalbumin Antibody - BSA Free [NB120-11427] - Female Ambra1+/− mice show reduced numbers of hippocampal PV interneurons. a Nissl staining of dorsal hippocampus coronal sections from WT & Ambra1+/− female mice (scale bar: 500 μm). b Subdivision of hippocampal layers (stratum oriens, CA1 pyramidal layer, stratum radiatum, stratum lacunosum moleculare, stratum moleculare, dentate gyrus upper blade, hilus, dentate gyrus lower blade), corresponding to the area depicted in a (scale bar: 100 μm). Ambra1+/− females show normal hippocampal layering (n = 3 mice per genotype). c Double labeling for Ambra1 (green) & PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 μm). The higher magnification image (scale bar, 5 μm) from the stratum pyramidale shows a PV neuron (right; merge in orange) & an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity & neuron borders are indicated by a dash line (n = 3 mice; three sections per animal). d, e Immunohistochemical labeling of PV in a coronal brain section of dorsal hippocampus obtained from female (d) & male (e) mice (scale bars: 500 μm). On the right are higher magnification images (scale bars: 50 μm). The plots show stereological quantification of PV interneurons (n = 6 mice per genotype & per gender; 14 sections per animal). The reduction in PV interneuron numbers is selective for female Ambra1+/− (two-tailed unpaired t test: *P = 0.043) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29488136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11427_rabbit-polyclonal-parvalbumin-antibody-310202416205149.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0014.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.")
![Immunohistochemistry: SCP3/SYCP3 Antibody [NB300-231] - Punctate staining of murine SCP3 in mouse ovary using NB300-231.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-NB300-231-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 and SCML2. (C-F) Immunostaining on meiotic chromosome spreads of FK2 and SYCP3 antibodies. Dotted circles are sex chromosomes and Scale bar are 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of D27C4 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A) Immunostaining of D27C4 and SYCP3 on wild-type meiotic chromosome spreads. Representative images are shown for each category through the early pachytene stage for spermatocytes from 9 independent mice. (C) Immunostaining of SYCP3 and D27C4 on Mdc1-KO meiotic chromosome spreads. Representative images are shown through the early pachytene stage for spermatocytes from 3 independent Mdc1-KO mice. (A and C) Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of E6C5 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A, B) Immunostaining of SCML2 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K4me2 and SYCP3 on meiotic chromosome spreads. Dotted circles: sex chromosomes. (E) Regulation of H3K4me2. (F, G) Immunostaining of H3K4me2 and SYCP3 on wild-type and Scml2-KO meiotic chromosome spreads. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K27ac and SYCP3 on meiotic chromosome spreads. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). (E) Model of the regulation of H3K27ac. (F) Immunostaining of SYCP3 and H3K27ac on wild-type and Rnf8-KO round spermatids. At least 30 round spermatids shown from at least 3 independent mice. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Mouse spermatocytes stained with biotin-conjugated anti-SYCP3 detected with streptavidin-Alexa Fluor 594 (left panel), streptavidin Alexa Fluor 488 (right panel). Image from verified customer review.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - SCP3 labeled in mouse pachytene preparation (red), using NB 300-231. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - CXXC1 is expressed in the spermatocytes in the presence or absence of PRDM9.(A) Immunofluorescence staining for CXXC1 & PRDM9 in adult B6, 14-dpp B6 & 14-dpp Prdm9-/- seminiferous tubule cross sections. Green, CXXC1; red, PRDM9; blue, DAPI. SC, Sertoli cell; Z, zygonema; P, pachynema; RS, round spermatid. Scale bars, first 4 columns: 50 μm, last column: 10 μm. (B) Immunofluorescence staining for CXXC1 & PRDM9 on chromosome spreads from adult B6. Green, SYCP3; orange, PRDM9; magenta, CXXC1. Scale bars, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415392592.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8 is required for polyubiquitination of the sex chromosomes: Immunostaining with E6C5 antibody, which recognizes polyubiquitination.(A-D) Immunostaining of SYCP3 & E6C5 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. (E) Schematic of ubiquitin targets recognized by the E6C5 antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415291958.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8-dependent H2AK119ub is removed by SCML2: Immunostaining with D27C4 antibody, which recognizes H2AK119ub.(A-D) Immunostaining of SYCP3 & D27C4 (H2AK119ub) on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among all images in this figure, Fig 3 & S2 Fig. (E) Updated model of distinct forms of regulation of ubiquitination by RNF8 & SCML2, including RNF8-dependent H2AK119ub, which is removed by SCML2 based on data in this figure. (F) Schematic of ubiquitin targets recognized by the D27C4 (H2AK119ub) antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205194.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 & SCML2: Immunostaining with FK2 antibody, which recognizes both mono- & polyubiquitinated conjugates.(A) Schematic of spermatogenesis. (B) Model of distinct forms of regulation of ubiquitination by RNF8 & SCML2. (C-F) Immunostaining of SYCP3 & FK2 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among the samples at the same stage. (G) Schematic of ubiquitin targets recognized by the FK2 antibody in each mouse model. A pink circle denotes RNF8-dependent ubiquitination & an orange circle denotes events mediated by a different E3 ubiquitin ligase. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212367.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - No major meiotic DSB repair or chromosome synapsis defects are observed in Cxxc1 CKO testis.(A) Immunostaining of SYCP3 & gamma H2AX on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, gamma H2AX. Scale bar, 10 μm. (B) Spermatocyte stage proportion in adult Cxxc1 het (n = 1,062 from two individuals) & CKO (n = 1,105 from two individuals) spermatocytes based on SYCP3/SYCP1/ gamma H2AX staining. p = 0.7 by Chi-square test. (C) Immunostaining of SYCP3 & SYCP1 on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; orange, SYCP1. Scale bar, 10 μm. (D) Crossover number measured by MLH1 staining on chromosome spreads of adult Cxxc1 het & CKO spermatocytes. Left, magenta, SYCP3; green, MLH1. Scale bar, 10 μm. Right, number of MLH1 foci per late pachynema in Cxxc1 het (n = 32 from two individuals) & CKO (n = 33 from two individuals). Bars represent mean ± SD. p = 0.4 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-3102024162188.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Meiotic spreads show deletion of Dicer1 disrupts progression of meiosis I.(A) It was possible to identify the 5 sub-stages of meiosis I by combining the reactivity patterns of two antibodies against synaptonemal complex protein 3 (SYCP3) & gamma H2AX. SYCP3 is a protein essential for synapsis of homologous chromosomes & gamma H2AX localizes to double-strand breaks & XY bodies during meiosis. A total of 200 spreads were counted in testis cell preparations from WT & GCKO mice at P22. (B) Chi square analysis shows GCKO testes contained significantly higher numbers of germ cell spreads at the leptotene & zygotene stages of meiosis I & fewer spreads at pachytene, diplotene & metaphase I stages (P<0.001), suggesting that the loss of Dicer1 lead to disruptions in progression through meiosis I. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23056286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212316.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - PRDM9 expression & catalytic function are not impaired in Cxxc1 CKO.(A) Immunostaining of PRDM9 shows unchanged pattern in adult CKO seminiferous tubules compared to the heterozygous control. Red, PRDM9; grey, DAPI. Scale bar, 20 μm. (B) PRDM9 & SYCP3 expression patterns are not changed in CXXC1 CKO chromosome spreads compared to the heterozygous control. Co-immunostaining of CXXC1 & PRDM9 on chromosome spreads from adult Cxxc1 het & CKO mice. Green, SYCP3; orange, PRDM9; magenta, CXXC1. First 4 rows, het control; last 4 rows, Cxxc1 CKO. Scale bar, 10 μm. (C) Meiosis progression occurs normally in Cxxc1 CKO testes. Immunostaining of H3K4me3 in adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, H3K4me3. Scale bar, 10 μm. (D) Testis-specific gene expression is not changed in Cxxc1 CKO testes. H3K4me3 ChIP-qPCR with chromatin isolated form Cxxc1 het & CKO mice. Promoter regions form Actinb & Sycp3, Dom2 hotspots PbxI & Fcgr4 were amplified. Cst hotspot HlxI was used as a negative control. Bars present mean ± SD of three biological replicates. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205179.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - DSB number is not affected in Cxxc1 CKO.The DSB number was determined by three markers reflecting different stages of their processing. (A) DMC1 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of DMC1 foci in early zygotene (n = 22 in het, n = 34 in CKO), late zygotene (n = 29 in het, n = 36 in CKO) & pachytene (n = 36 in het, n = 26 in CKO) spermatocytes. (B) RAD51 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RAD51 foci in early zygotene (n = 22 in het, n = 13 in CKO), late zygotene (n = 24 in het, n = 11 in CKO) & pachytene (n = 52 in het, n = 36 in CKO) spermatocytes. (C) RPA staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RPA foci in early zygotene (n = 35 in het, n = 43 in CKO), late zygotene (n = 33 in het, n = 28 in CKO) & pachytene (n = 89 in het, n = 92 in CKO) spermatocytes. For A-C, two individuals per genotype were measured. Bars represent mean ± SD. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416203720.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0014.jpg "Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license.")
![Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0011.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-2379 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2379-img0018.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and Western blot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0016.jpg)
![Immunohistochemistry-Paraffin: OCT4 Antibody [NB100-2379] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of OCT4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/OCT4-Antibody-Immunohistochemistry-Paraffin-NB100-2379-img0017.jpg)
![Flow Cytometry: OCT4 Antibody - BSA Free [NB100-2379] - An intracellular stain was performed on U2-OS cells with OCT4 NB100-2379AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/OCT4-Antibody---BSA-Free-Flow-Cytometry-NB100-2379-img0022.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.](http://images.novusbio.com/fullsize/OCT4-Antibody-Western-Blot-NB100-2379-img0013.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0019.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Neuro2a cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0020.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Ntera2 cells with OCT4 Antibody NB100-2379 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0021.jpg)
![Western Blot: OCT4 Antibody - BSA Free [NB100-2379] - Protein expression of NANOG a, OCT4 b & SOX2 c in bovine uterine tissues obtained from control cows & from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal & adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 & GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2379_rabbit-polyclonal-oct4-antibody-310202415394524.jpg)
![Western Blot: OCT4 Antibody - BSA Free [NB100-2379] - Protein expression of pluripotency markers in uterine cells isolated from control cows & from cows with adenomyosis. NANOG a OCT4 b & SOX2 c protein expression in uterine stromal cells. NANOG d OCT4 e & SOX2 f protein expression in uterine myometrial cells. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. Asterisks indicate statistical differences between uterine normal & adenomyotic tissue (*P < 0.05; **P < 0.01), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 & GAPDH are shown below the graphs g. MM – molecular weight marker, C – cells obtained from control cows, ADENO – cells obtained from cows with adenomyosis Image collected & cropped by CiteAb from the following publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2379_rabbit-polyclonal-oct4-antibody-310202415384144.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0002.jpg "Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.")
![Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/DIO3-Antibody-Flow-Cytometry-NBP1-05767-img0005.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0003.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Western-Blot-NB600-1160-img0013.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunocytochemistry-Immunofluorescence-NB600-1160-img0012.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunohistochemistry-Paraffin-NB600-1160-img0004.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Flow-Cytometry-NB600-1160-img0011.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Western-Blot-NB600-1160-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Immunocytochemistry-Immunofluorescence-NB600-1160-img0005.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Simple-Western-NB600-1160-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-31A3-Simple-Western-NB600-1160-img0010.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, NB110-58872, dilution 1:2,000 in green, and mouse mAb to Actin, dilution 1:1,000, in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58872-img0002.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of equal amounts of different tissue and cell lysates using chicken pAb to UCHL1, NB110-58872, dilution 1:2,000 in green, and mouse mAb to Actin, dilution 1:1,000, in red: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa and [7] SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - (A) Immunolabeling of the neuronal marker UCHL1 and TRPV1 in frozen DRG sections and cultured sensory neurons from rat. TRPV1 is selectively expressed in a subpopulation of sensory neurons. (B) Representative view field showing the automated image analysis to quantify TRPV1 expression in cultured sensory neurons. Green encircled objects represent sensory neurons marked by UCHL1. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0115731), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58872-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to UCHL1, NB110-58872 , dilution 1:500 in red, and costained with mouse mAb to vimentin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58872-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416212311.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - PGD2 is a paracrine mediator synthesized in myelinated large-diameter neurons acts on TRPV1(+) neurons.(A) Dose-dependent induction of RII phosphorylation in sensory neurons after 1 min stimulation w/ PGD2 (EC50 = 377 nM, n = 3,>2000 neurons/condition; one-way ANOVA w/ Bonferroni's multiple comparisons test). (B) PGD2 did not induce pRII in non-neuronal cells of same cultures shown in A. (C) Time course of RII phosphorylation indicating long-lasting effects of PGD2 (10 µM) on sensory neurons. (D) Stimulation w/ PGD2 also results in phosphorylation of ERK1/2 measured in same cultures shown in D. (E) Representative experiment demonstrating induction of RII phosphorylation is enhanced in TRPV1(+) neurons (total of 3664 neurons). Plots of respective controls are shown in S2 Fig. (F) Fold changes of pRII intensities in TRPV1(−) (grey bars) & TRPV1(+) (black bars) neurons after 1 min stimulation w/ 10 µM PGD2 (n = 3,>2000 neurons/condition, one-way ANOVA w/ Bonferroni's multiple comparisons test). (G) Co-labeling of TRPV1 & PTGDS revealing PTGDS is expressed in neurons lacking TRPV1 (total of 9951 neurons, also refer to S2 Fig. for control plots). (H) Co-labeling of NF200 & PTGDS showing PTGDS(+) neurons express NF200 (total of 12966 neurons, also refer to S2 Fig. for control plots).(I) Size distribution of PTGDS(+) (green), NF200(+) (red), & all sensory neurons (black) indicating PTGDS(+) neurons are larger than other neurons. (J) Suggested pathway of interneuronal communication between subgroups of sensory neurons. Large-diameter mechanosensitive neurons express PTGDS resulting in production of PGD2, which activates DP1 receptors present on nociceptive neurons expressing TRPV1. Image collected & cropped by CiteAb from following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241621833.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241620379.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416235521.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-31020241623555.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-3102024162498.jpg)
![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58872] - Validation of the transcriptome data by single cell based quantitative High Content Screening (HCS) microscopy focusing on selected signaling-relevant proteins.(A) Triple staining of the neuronal marker UCHL1 & two different TRPV1 antibodies derived from rabbit & goat, respectively, to facilitate the analysis of various targets. The staining intensities obtained with both TRPV1 antibodies correlated significantly (Spearmans ρ = 0.96, p3000 analyzed neurons per experiment, paired two-tailed t-tests). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58872_chicken-polyclonal-uch-l1-pgp9-5-antibody-310202416235515.jpg)